Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,387	C→G	intergenic (+277/‑34)	pykF → / → lpp	pyruvate kinase I/murein lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,387	0	C	G	100.0%	64.3 / NA	18	intergenic (+277/‑34)	pykF/lpp	pyruvate kinase I/murein lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (6/12);  total (6/12)

TATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTAAACTGGTACTGGGCGCG  >  NC_000913/1757345‑1757450                                           |                                                                tatTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAggg                                        <  2:319245/68‑1 (MQ=255)    tctcAACATAAAAAACTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGTGGGTAt                                     >  1:234550/1‑68 (MQ=255)    tctcAACATAAAAAACTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTAt                                     >  1:60110/1‑68 (MQ=255)     ctcAACATAAAAAACTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTAtt                                    <  1:200390/68‑1 (MQ=255)       cAACATAAAAAACTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATtaa                                  <  1:301648/68‑1 (MQ=255)                  aCTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAgg                                         <  2:705520/50‑1 (MQ=255)                  aCTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAgg                                         >  1:705519/1‑50 (MQ=255)                  aCTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGct                       <  1:402209/68‑1 (MQ=255)                  aCTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGct                       <  2:50469/68‑1 (MQ=255)                  aCTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGct                       <  2:435962/68‑1 (MQ=255)                  aCTTTGTGTAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGct                       <  1:524909/68‑1 (MQ=255)                        tgtAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTaaa                 <  1:319921/68‑1 (MQ=255)                        tgtAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTaaa                 <  1:195234/68‑1 (MQ=255)                         gtAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTaaa                 <  1:354740/67‑1 (MQ=255)                         gtAATACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTaaa                 >  2:354741/1‑67 (MQ=255)                             tACTTGTAACGCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTAAACTGGt            <  1:187217/68‑1 (MQ=255)                              aCTTGAAGCGCGAGATGGAGATTAAAACAATCTAGAGGGTATTAATAATGAAAGCTTCGTAACTggag           >  1:188481/1‑66 (MQ=255)                                       gCTAGATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTAAACTGGTACTGGGcgcg  >  1:366906/1‑68 (MQ=255)                                           |                                                                TATTCTCAACATAAAAAACTTTGTGTAATACTTGTAACGCTACATGGAGATTAACTCAATCTAGAGGGTATTAATAATGAAAGCTACTAAACTGGTACTGGGCGCG  >  NC_000913/1757345‑1757450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑