| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,563,398 | 0 | G | C | 41.7% | 24.9 / 11.8 | 24 | S779C (TCC→TGC) | dosP | oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator |
| Reads supporting (aligned to +/- strand): ref base G (9/5); new base C (0/10); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTT > NC_000913/1563332‑1563463 | tGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGa < 2:804891/68‑1 (MQ=255) tttCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAat > 1:324741/1‑68 (MQ=255) cAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATc < 1:340989/68‑1 (MQ=255) aGCGGTAACACGCTGCTCATCCAGCCTGTAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATcc < 2:862092/68‑1 (MQ=255) aGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATcc > 2:80246/1‑68 (MQ=255) acGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAACAAACACCCCTGAATAAc < 2:702972/68‑1 (MQ=255) gctgctCATCCGGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgc > 2:658695/1‑68 (MQ=255) gctgctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgc > 1:972935/1‑68 (MQ=255) gctgctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgc > 1:472972/1‑68 (MQ=255) gctCATCCAGCCTGTAATTTCTTCGGCTGGTAGGGGTCTGCAAAAGAATTATCCCTGATTAACGCTAc < 1:711214/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGAc < 1:613359/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGAc < 2:472972/68‑1 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGAc < 2:53317/68‑1 (MQ=255) aTCCAGCCTGTAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAGTg < 1:88825/68‑1 (MQ=255) tCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGAgg > 2:574231/1‑68 (MQ=255) cAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGAt > 2:956394/1‑68 (MQ=255) cAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAGTGGAt < 1:834778/68‑1 (MQ=255) ctccttcGGCGGGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGc < 2:415965/65‑1 (MQ=255) tcttcGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAGCGCGACAGTGGATCTTGCGTAGCAt > 2:587555/1‑68 (MQ=255) cggcggGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCa > 1:90530/1‑68 (MQ=255) ggcggGTAGGGGGCGGGATAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCaa < 2:294144/68‑1 (MQ=255) ggcggGTAGGGGGCGGCAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCaa < 2:923704/68‑1 (MQ=255) gCGGCAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTgg < 2:101591/68‑1 (MQ=255) cGGCAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGt < 2:98363/68‑1 (MQ=255) gggAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGtt < 1:574231/68‑1 (MQ=255) | TGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTT > NC_000913/1563332‑1563463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |