Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,413,571	T→C	intergenic (‑106/‑293)	acrS ← / → acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,413,571	0	T	C	100.0%	38.3 / NA	12	intergenic (‑106/‑293)	acrS/acrE	acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/8);  total (4/8)

AATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAAT  >  NC_000913/3413510‑3413629                                                              |                                                           aaTAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAAt                                                      >  2:956279/1‑68 (MQ=255)  aTAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATc                                                     <  1:863940/68‑1 (MQ=255)  aTAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATc                                                     <  1:956282/68‑1 (MQ=255)       gAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaa                                                <  1:843048/68‑1 (MQ=255)           gTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTTAGTTGCCATAAATCGTtaaataa                                            <  1:482548/68‑1 (MQ=255)                      aCCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                            <  1:741175/57‑1 (MQ=255)                      aCCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCCATAAATCGTtaaataa                                            >  2:741173/1‑57 (MQ=255)                                      taCGAGAGGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATAc                 >  1:293599/1‑68 (MQ=255)                                           gagGCTAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCa            <  2:777605/68‑1 (MQ=255)                                                tAATATTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACa       <  1:631496/68‑1 (MQ=255)                                                    atTGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCaa   >  2:288034/1‑68 (MQ=255)                                                     ttGAGTTGCCATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAat  <  2:728753/68‑1 (MQ=255)                                                              |                                                           AATAGCGAAGGTTAATCTATCACCTAATGTGTATTTATACGAGAGGCTAATATTGAGTTGCTATAAATCGTTAAATAAATAATATATATTATTTACCTAAGATACATTCACTACATCAAT  >  NC_000913/3413510‑3413629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑