Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,796	G→T	E367* (GAA→TAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,796	0	G	T	100.0%	54.0 / NA	18	E367* (GAA→TAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/9);  total (9/9)

AACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACG  >  NC_000913/1756733‑1756860                                                                |                                                                 aacaaTGACAACCGTAAACTGCGCATTACCTAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTTaaaa                                                              <  2:668691/68‑1 (MQ=255)        aCAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGGTGAAACTGCTTAAAAACTGGAt                                                       <  1:216973/68‑1 (MQ=255)           aCCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTTAAAAACTGGATGCt                                                    >  2:469376/1‑68 (MQ=255)             cGTATACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTTAAAAACTGGATGCTcc                                                  <  2:297119/68‑1 (MQ=255)             cGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTTAAAAACTGGATGCTcc                                                  <  2:582312/68‑1 (MQ=255)                  aCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGGGGCTGCTTAAAAACTGGATGCTGCGCTgg                                             >  2:919477/1‑67 (MQ=255)                  aCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTTAAAAACTGGATGCTCCGCTGa                                             >  1:56467/1‑68 (MQ=255)                  aCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTTAAAAACTGGATGCTCCGCTGa                                             >  2:736386/1‑68 (MQ=255)                   cTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAGCTGCTTTAAAACTGGATGCTCCGCTGAt                                            >  2:766163/1‑68 (MQ=255)                   cTGCGCATTACCGAAGCGGTATGCCGTGGTGACGTTGAAACTGCTTAAAAACTGGATGCTCCGCTGAt                                            >  1:426689/1‑68 (MQ=255)                          ctACCGAAGCGGTATGCCGTGGTGCCGCCGAAACTGCTTAAAAACTGGATGCTCCGCCGATCGCggcc                                     <  2:72204/67‑3 (MQ=255)                               gAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTTAATAACTGGATGCTCCGCTGATCGTGGTTGCTAc                                <  2:745737/68‑1 (MQ=255)                                   cGGTATGCCGTGGTGCCGTTGAAACTGCTTAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAg                            >  2:919189/1‑68 (MQ=255)                                         gCCGTGGTGCCGTTGAAACTGCTTAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGt                      >  2:350763/1‑68 (MQ=255)                                            gtggtgCCGTTGAAACTGCTTAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTaaa                   >  1:308520/1‑68 (MQ=255)                                                          aCTGCTTAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGt     <  2:71165/68‑1 (MQ=255)                                                          aCTGCTTAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGt     <  1:625379/68‑1 (MQ=255)                                                          aCTGCTTAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGt     <  1:518038/68‑1 (MQ=255)                                                           ctgcttAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTa    <  1:875002/68‑1 (MQ=255)                                                           ctgcttAAAAACTGGATGCTCCGCTGAGCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTa    <  2:332540/68‑1 (MQ=255)                                                           ctgcttAAAAACTGGACGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAACCTGCCCGCGCAGTa    <  2:946269/68‑1 (MQ=255)                                                             gcttAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACg  <  1:888601/68‑1 (MQ=255)                                                                |                                                                 AACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACG  >  NC_000913/1756733‑1756860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑