Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,590	G→A	Q54* (CAG→TAG)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,590	0	G	A	100.0%	96.7 / NA	29	Q54* (CAG→TAG)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/18);  total (11/18)

GCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATC  >  NC_000913/2312525‑2312653                                                                  |                                                                gCCGTAAACGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGt                                                               >  1:47583/1‑68 (MQ=255)   cGTAACCGGTCAGCTGGTCAGCAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCg                                                             <  2:693961/68‑1 (MQ=255)       gccGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCAt                                                         >  2:52018/2‑68 (MQ=255)       gcaggTCAGCTGGTCAGGAACATGAGTTTCACCTAAGAAGCCAAGACGCATGTAGGAATAGTCGCAAt                                                         >  2:840314/4‑68 (MQ=255)       aCCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCAt                                                         <  2:766149/68‑1 (MQ=255)           gTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTAc                                                     <  1:132378/68‑1 (MQ=255)               gCTGGTCAGTGACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACGTCt                                                 >  2:735524/1‑68 (MQ=38)               gCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCt                                                 >  2:889430/1‑68 (MQ=255)                        tAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCa                                        <  1:565996/68‑1 (MQ=255)                          aCCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCaga                                      >  1:296226/1‑68 (MQ=255)                            cTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAgaga                                    >  1:565516/1‑68 (MQ=255)                               aGTTTCACCTTTGAAGCCAAGACGCATGAAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAAt                                 >  2:677460/1‑68 (MQ=255)                                     aCCTTTGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCa                           >  1:239366/1‑68 (MQ=255)                                        tttGAAGCCAAGACGCATGTAGGTCTAGTCGCCGTCTACATCTTTGTTGTCAGAGAAATAGTGCAGGc                        <  1:576372/68‑1 (MQ=255)                                        tttGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGc                        <  1:72274/68‑1 (MQ=255)                                        tttGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGc                        <  1:256050/68‑1 (MQ=255)                                        tttGAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGCGCAggt                        <  2:613011/68‑2 (MQ=255)                                           gAAGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGt                     <  1:41034/68‑1 (MQ=255)                                             aGCCAAGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCt                   >  2:413893/1‑68 (MQ=255)                                                  aGACGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACttt              <  1:667941/68‑1 (MQ=255)                                                    aCGCATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTAc            <  2:889989/68‑1 (MQ=255)                                                       cATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAATTAGCGCAGGCCGTCTACTTTACCGt         <  1:860603/68‑1 (MQ=255)                                                       cATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGt         <  1:837368/68‑1 (MQ=255)                                                       cATGTAGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGt         <  1:413893/68‑1 (MQ=255)                                                            tggTCTAGTCTCCATCTTCATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCTTACata    <  1:840317/67‑3 (MQ=255)                                                            aGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa    <  2:296226/68‑1 (MQ=255)                                                            aGGTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa    >  1:418556/1‑68 (MQ=255)                                                            aGGTCTAGTCGCCATCTACATCTTTGTTGGCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGa    <  2:717556/68‑1 (MQ=255)                                                              gTCTAGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATc  <  2:239366/68‑1 (MQ=255)                                                                  |                                                                GCCGTAACCGGTCAGCTGGTCAGTAACCTGAGTTTCACCTTTGAAGCCAAGACGCATGTAGGTCTGGTCGCCATCTACATCTTTGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATC  >  NC_000913/2312525‑2312653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑