breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G intergenic (+123/‑1156) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 547,835 +G intergenic (+264/‑1015) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolatedehydrogenase/5,10‑methylene ‑tetrahydrofolatecyclohydrolase
MC JC 654,214 Δ1,199 bp insH_12 insH_12
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,093,686 T→C V130A (GTA→GCA)  ycdT → diguanylate cyclase
RA 1,197,797 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,809 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,822 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase,‑specific for NADP+
RA 1,197,824 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase,‑specific for NADP+
MC JC 1,301,041 Δ1,336 bp insC_8insD_10 insC_8, insD_10
RA 1,519,222 G→T V40V (GTG→GTT ydcX → inner membrane protein
RA 1,669,601 Δ1 bp coding (678/1221 nt) dgsA ← DNA‑binding transcriptional repressor
RA 1,979,957 G→T intergenic (‑37/+398) flhD ← / ← insH_10 DNA‑binding transcriptional dual regulator/IS5 element protein
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
MC JC 2,176,983 Δ1,199 bp insH_11 insH_11
RA 2,245,455 A→G S283P (TCG→CCG)  yeiB ← inner membrane protein
MC JC 2,320,795 Δ1,336 bp insD_9insC_5 insD_9, insC_5
RA 2,866,111 A→G intergenic (‑21/+159) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/outer membrane lipoprotein
MC JC 3,261,134 Δ1,199 bp insH_16[tdcD] insH_16, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  yhaC → hypothetical protein
MC JC 3,742,936 Δ1,336 bp insD_8insC insD_8, insC
MC JC 3,746,911 Δ1,199 bp insH_13 insH_13
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) dctA → C4‑dicarboxylic acid, orotate and citratetransporter
RA 4,371,274 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779_cfb 1197836 1213064 15229 46 [45] [45] 47 [icd]–mcrA [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,cohE,croE,ymfL,ymfM,ymfN,ymfR,ymfO,ymfP,ymfQ,stfP,tfaP,tfaE,pinE,mcrA
* * ÷ NC_007779_cfb 4310268 4311647 1380 47 [45] [45] 46 insH_15–[alsK] insH_15,[alsK]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779_cfb 380484 =NA (NA)97 (0.900) 87/436 0.4 NA intergenic (‑418/‑46) yaiO/insC_2 hypothetical protein/IS2 insertion element repressor InsA
?NC_007779_cfb = 381407 NA (NA)coding (510/906 nt) insD IS2 insertion element transposase InsAB'
* ? NC_007779_cfb 381401 =NA (NA)124 (1.160) 106/432 0.1 NA coding (504/906 nt) insD IS2 insertion element transposase InsAB'
?NC_007779_cfb = 381812 NA (NA)coding (303/387 nt) yaiF hypothetical protein
* ? NC_007779_cfb = 3186081NA (NA)112 (1.040) 93/434 0.2 97.4% coding (26/2466 nt) yqiG outer membrane usher protein
?NC_007779_cfb 3512753 = 3 (0.030)coding (529/1026 nt) cytR DNA‑binding transcriptional dual regulator
* ? NC_007779_cfb = 35127591 (0.010)90 (0.840) 80/434 0.5 98.9% coding (535/1026 nt) cytR DNA‑binding transcriptional dual regulator
?NC_007779_cfb = 3744262 NA (NA)intergenic (‑90/+31) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
* ? NC_007779_cfb = 3744263NA (NA)109 (1.010) 94/436 0.2 94.8% intergenic (‑91/+30) insC/yidZ IS2 element protein/DNA‑binding transcriptional regulator
?NC_007779_cfb 3903274 = 6 (0.060)coding (37/825 nt) bax hypothetical protein
* ? NC_007779_cfb = 37505900 (0.000)96 (0.910) 88/428 0.3 100% intergenic (‑104/‑69) tnaA/insH_14 tryptophanase/L‑cysteine desulfhydrase,PLP‑dependent/IS5 element protein
?NC_007779_cfb 3751790 = 0 (0.000)intergenic (+151/+116) insH_14/tnaC IS5 element protein/tryptophanase leader peptide
* ? NC_007779_cfb = 39032792 (0.020)114 (1.060) 97/434 0.2 98.3% coding (42/825 nt) bax hypothetical protein
?NC_007779_cfb = 4504190 NA (NA)intergenic (+10/‑89) insD_3/yjgW IS2 insertion element transposase InsAB'/hypothetical protein
* ? NC_007779_cfb = 4311556NA (NA)110 (1.020) 83/434 0.5 99.1% coding (922/1029 nt) alsK D‑allose kinase
?NC_007779_cfb 4540915 = 1 (0.010)intergenic (+204/+379) yjhR/nanS frameshift suppressor/hypothetical protein