BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779_cfb	62,127	+G	coding (1138/2907 nt)	rapA ←	RNA polymerase‑associated helicase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779_cfb	62,126	1	.	G	98.6%	393.3 / ‑1.1	139	coding (1139/2907 nt)	rapA	RNA polymerase‑associated helicase protein
Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (68/69); total (70/69)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

CGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCT‑GCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAAT  >  NC_007779_cfb/61877‑62370

tgATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACACGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCt                                                                                                                                                                                                                                                       <  1:1200969/249‑1 (MQ=255)

cGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCt                                                                                                                                                                                                                                                       >  1:543381/1‑250 (MQ=255)

   tCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCT‑ggc                                                                                                                                                                                                                                                   <  2:543381/250‑3 (MQ=255)

      tCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCt                                                                                                                                                                                                                                                       <  1:376760/244‑1 (MQ=255)

              tttACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGatcat                                                                                                                                                                                                                                         >  1:774835/1‑250 (MQ=255)

                 aCGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCAtctc                                                                                                                                                                                                                                      >  1:1127649/1‑250 (MQ=255)

                 aCGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCAtctc                                                                                                                                                                                                                                      <  1:204100/250‑1 (MQ=255)

                  cGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCg                                                                                                                                                                                                                                     <  1:1250537/250‑1 (MQ=255)

                      cgcCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCCCGCCGa                                                                                                                                                                                                                                 >  1:1481965/1‑250 (MQ=255)

                                   ggAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGt                                                                                                                                                                                                                           <  2:1130455/243‑1 (MQ=255)

                                   ggAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGt                                                                                                                                                                                                                           >  1:1130455/1‑243 (MQ=255)

                                    gagaCTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCGTCGTAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCCATACC‑CCG‑GCTAGGCGAACACACCGCCGAGCATGGTCacgtcg                                                                                                                                                                                                                   >  2:596658/1‑245 (MQ=255)

                                       aCTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCa                                                                                                                                                                                                                <  1:1635849/250‑1 (MQ=255)

                                         tttAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCAtt                                                                                                                                                                                                              >  1:513611/1‑250 (MQ=255)

                                                   gTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGGTA‑TCCCG‑GCTCGCCGTTCATCTCCCCGAGCATGGTCCGTTCGTCATTGCTCAGTTTg                                                                                                                                                                                                    >  2:761362/1‑250 (MQ=255)

                                                      ctcttccgatctggTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACAGTTACGCGTGTTACGGGGCGGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTa                                                                                                                                                                                                 <  1:1362483/238‑1 (MQ=255)

                                                        aTACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCAAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTAcc                                                                                                                                                                                               <  1:85407/250‑1 (MQ=255)

                                                          ccgtcGTCNGTGGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTg                                                                                                                                                                                             <  2:903776/246‑1 (MQ=255)

                                                            tGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGcc                                                                                                                                                                                           >  2:155058/1‑250 (MQ=255)

                                                            tGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGACGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGcc                                                                                                                                                                                           >  1:137847/1‑250 (MQ=255)

                                                                  ggTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCagcagc                                                                                                                                                                                     >  1:1195487/1‑250 (MQ=255)

                                                                   gTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAgcagca                                                                                                                                                                                    >  1:608673/1‑250 (MQ=255)

                                                                          cAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAAc                                                                                                                                                                             <  1:1355588/250‑1 (MQ=255)

                                                                             cTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAGCGGc                                                                                                                                                                          <  2:185463/250‑1 (MQ=255)

                                                                             cTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGc                                                                                                                                                                          <  1:596658/250‑1 (MQ=255)

                                                                             cTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGc                                                                                                                                                                          <  2:770608/250‑1 (MQ=255)

                                                                              ttAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCg                                                                                                                                                                         <  1:155058/250‑1 (MQ=255)

                                                                                     tgtgCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCaa                                                                                                                                                                  <  2:726636/250‑1 (MQ=255)

                                                                                       tgCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAAcc                                                                                                                                                                <  1:761362/250‑1 (MQ=255)

                                                                                          aGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTGCGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCTGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGa                                                                                                                                                             >  2:385604/1‑250 (MQ=255)

                                                                                             tCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCa                                                                                                                                                                                                                              <  1:61370/182‑1 (MQ=255)

                                                                                             tCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCg                                                                                                                                                               >  1:1394768/1‑245 (MQ=255)

                                                                                             tCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGCGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCa                                                                                                                                                                                                                              >  2:61370/1‑182 (MQ=255)

                                                                                             tCGCGTTTCGGGAATCCTTTCACACCGGTACGCGTGTTACGGAATAGCACGCGGATCGGGCGGTTGCGATCCATCAGCATCGAAACCAGCTTCTGACGGGCGCTCAGGGCATTTTCGGGGCCGCTGTTTGCTTCCTGCAACGGCGGGCCGATT‑TACTT‑GCACTCCGATCAACTTGCCGAGCAACTTCCGTTCAGCATTGCCCAGGTTGTGAACTAGCAGCTACATGGCTACGGCCTCCGCACTCGGACgt                                                                                                                                                          >  2:1689927/1‑249 (MQ=255)

                                                                                                 gTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAAt                                                                                                                                                      <  1:1727725/250‑1 (MQ=255)

                                                                                                       ggAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGACGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCt                                                                                                                                                <  2:137847/250‑1 (MQ=255)

                                                                                                             cTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCtt                                                                                                                                          >  1:70236/1‑250 (MQ=255)

                                                                                                              tttNCNNCCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTc                                                                                                                                         <  2:1195487/250‑1 (MQ=255)

                                                                                                              tttCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTc                                                                                                                                         >  1:835049/1‑250 (MQ=255)

                                                                                                              tttCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTc                                                                                                                                         >  2:1357455/1‑250 (MQ=255)

                                                                                                               ttCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGc                                                                                                                                              <  1:646206/244‑1 (MQ=255)

                                                                                                                tCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAAGCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCaa                                                                                                                                       >  2:336941/1‑250 (MQ=255)

                                                                                                                   gcccGTTACGCGTGTTACGGAACAGCCAGCGGCTGGTGCCGTGGCGGTCCGTCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAAAGa                                                                                                                                    <  2:1552998/248‑1 (MQ=255)

                                                                                                                    acCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGaa                                                                                                                                   <  2:608673/250‑1 (MQ=255)

                                                                                                                              gtgtTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGtt                                                                                                                                                                                                      >  1:550661/1‑173 (MQ=255)

                                                                                                                                gtTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGttt                                                                                                                                                                                                     <  1:1331900/172‑1 (MQ=255)

                                                                                                                                gtTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGttt                                                                                                                                                                                                     >  2:1331900/1‑172 (MQ=255)

                                                                                                                                  tACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTc                                                                                                                                                                                                                    >  1:109756/1‑155 (MQ=255)

                                                                                                                                  tACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTc                                                                                                                                                                                                                    <  2:109756/155‑1 (MQ=255)

                                                                                                                                         cAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACg                                                                                                              >  1:245422/1‑250 (MQ=255)

                                                                                                                                          aGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCaa                                                                                                                                       >  2:985706/1‑224 (MQ=255)

                                                                                                                                           gCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTACTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCCGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCa                                                                                                                                                                                                         <  1:156323/157‑1 (MQ=255)

                                                                                                                                           gCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTACTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCCGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCa                                                                                                                                                                                                         >  2:156323/1‑157 (MQ=255)

                                                                                                                                            cACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGtt                                                                                                           <  1:1268185/250‑1 (MQ=255)

                                                                                                                                            cACCCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTCACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATa                                                                                                                                                        >  2:981386/1‑205 (MQ=255)

                                                                                                                                            cACCCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTCACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATa                                                                                                                                                        <  1:981386/205‑1 (MQ=255)

                                                                                                                                               gcgGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTTGTCTTCAACGAACTGCGCAAAATCGTGGCAACGGTTCgg                                                                                                        >  2:561994/1‑250 (MQ=255)

                                                                                                                                                 ggCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCCGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGt                                                                                                      >  2:1652178/1‑250 (MQ=255)

                                                                                                                                                 ggCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAGCGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGa                                                                                                                                    >  1:737951/1‑220 (MQ=255)

                                                                                                                                                    tGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAAc                                                                                                                                                                             >  1:266766/1‑176 (MQ=255)

                                                                                                                                                    tGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAAc                                                                                                                                                                             <  2:266766/176‑1 (MQ=255)

                                                                                                                                                       tGCCGTGGCGATCCATCAGCATCGAGACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑TCCCG‑GCTCGCCGATCATCTCGCCGAGCATGTTCGGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGCCCGCAAGCGGACGATAATTTTTCTGCTCTTCTATGAATTGCGCAAAATAGTGGAAACGGTTCGGGNNcngta                                                                                                >  2:1219631/1‑248 (MQ=255)

                                                                                                                                                            tGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAgcagacgc                                                                                           >  2:719165/1‑250 (MQ=255)

                                                                                                                                                               cGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGtt                                                                                                                                                                                                  >  1:211797/1‑144 (MQ=255)

                                                                                                                                                               cGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGtt                                                                                                                                                                                                  <  2:211797/144‑1 (MQ=255)

                                                                                                                                                                gATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAgcagacgcagac                                                                                       >  1:93897/1‑250 (MQ=255)

                                                                                                                                                                 aTCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGcagacgcagacg                                                                                      <  2:835049/250‑1 (MQ=255)

                                                                                                                                                                  tCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGATCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACgg                                                                                     <  2:1265543/250‑1 (MQ=255)

                                                                                                                                                                   ccATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCg                                                                                                                                                               >  2:540748/1‑175 (MQ=255)

                                                                                                                                                                   ccATCAGCATCGAAACCAGCTACTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCATGCAACAGCGGCTCGATA‑T‑CCTGGCTCGTCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAACATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACggg                                                                                    <  1:1689927/250‑1 (MQ=255)

                                                                                                                                                                      tCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGa                                                                                                                                                          <  2:1723514/177‑1 (MQ=255)

                                                                                                                                                                             cGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAAc                                                                                                                                      >  2:1212267/1‑190 (MQ=255)

                                                                                                                                                                                   cAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCAt                                                                    >  1:587459/1‑250 (MQ=255)

                                                                                                                                                                                    aGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATc                                                                   >  2:510739/1‑250 (MQ=255)

                                                                                                                                                                                       tCCTGACGGTCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCCCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCCGCAGACGCAGTCGGGCGTAGAGGCTGTCCTTcgcc                                                                >  2:685279/1‑250 (MQ=255)

                                                                                                                                                                                                tctcccnGGGCATCTTCGCTGTCGCTGTTTGCTGCCTCCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTcc                                                       <  2:1415355/245‑1 (MQ=255)

                                                                                                                                                                                                   ctctGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTccaga                                                    >  1:1415355/1‑247 (MQ=255)

                                                                                                                                                                                                          cATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAgcagacgcaga                                                                                        >  1:1313644/1‑207 (MQ=255)

                                                                                                                                                                                                          cATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAgcagacgcaga                                                                                        <  2:1313644/207‑1 (MQ=255)

                                                                                                                                                                                                           aTCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCTCCGAGCATGTTCCGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACCGCGTCCGCAACCGGACGATAATTTTTCTCCTCTTCCACGATCTGCGCTAATTCGTGGAAACGGTTCGGGTCCAGCAGACGCAGTCGTGCGCAGTGGCTTTCCCTTCCCCTCTGTTCCGGGTTNCCggn                                            >  2:1016421/1‑249 (MQ=255)

                                                                                                                                                                                                                cgctgtcgctgtTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCGGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCAATCCCCAGCTGTTCCGGGGTCGCGGTcagca                                       <  1:1219631/250‑1 (MQ=255)

                                                                                                                                                                                                                 gctgtcgctgtTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCGTGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGt                                                                                                            <  2:1393050/180‑1 (MQ=255)

                                                                                                                                                                                                                          gtTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGcc                             <  1:999753/250‑1 (MQ=255)

                                                                                                                                                                                                                                tgcctgcAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTc                                                                                                          >  2:1511119/1‑167 (MQ=255)

                                                                                                                                                                                                                                  cctgcAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTgcg                                                                                                                              >  1:672260/1‑145 (MQ=255)

                                                                                                                                                                                                                                  cctgcAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTgcg                                                                                                                              <  2:672260/145‑1 (MQ=255)

                                                                                                                                                                                                                                      cAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTcgc                                               >  2:1468624/1‑220 (MQ=255)

                                                                                                                                                                                                                                      cAACAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTcgc                                               <  1:1468624/220‑1 (MQ=255)

                                                                                                                                                                                                                                       aaCAGCGGCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTGCCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGACCTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCTGCCGCATAACGTCCGGCAAGtagctc                >  2:828240/1‑245 (MQ=255)

                                                                                                                                                                                                                                          tccgatctgATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGcc             <  1:1738923/242‑1 (MQ=255)

                                                                                                                                                                                                                                              gCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTcc                                                                                                    >  2:467486/1‑159 (MQ=255)

                                                                                                                                                                                                                                              gCTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTcc                                                                                                    <  1:467486/159‑1 (MQ=255)

                                                                                                                                                                                                                                               cTCGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCGGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGcc                             <  2:1108189/229‑1 (MQ=255)

                                                                                                                                                                                                                                                 cGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGGCGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTcagcag                                      <  2:38201/218‑1 (MQ=255)

                                                                                                                                                                                                                                                 cGATA‑T‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGGCGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTcagcag                                      >  1:38201/1‑218 (MQ=255)

                                                                                                                                                                                                                                                  gATA‑T‑CCTGGCTCGCCGATCGTCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTTCAGCAGACGCAGACGGGCGAAGTGGGTTTGCATCCCGAGCTGTTCCGGGGTCGCGGTCACCAGCAGGACGGCCGCCACGGGCTCTGCCAgatcgga     >  2:1738923/1‑244 (MQ=255)

                                                                                                                                                                                                                                                   ata‑t‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCcagcag                                                                                               >  2:1005707/1‑159 (MQ=255)

                                                                                                                                                                                                                                                   ata‑t‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCcagcag                                                                                               <  1:1005707/159‑1 (MQ=255)

                                                                                                                                                                                                                                                     a‑t‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTcagca                                       >  1:1076819/1‑213 (MQ=255)

                                                                                                                                                                                                                                                     a‑t‑CCTGGCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAAt  >  2:863888/1‑250 (MQ=255)

CGATCTTCCGCACTTTTACGTGCGCCCATAATGCCGGAGACTTTAATAGCCGTCTGATACTGCGTCGGTAGCGGCAGCTTAATGGTGTGCAGCTCGCGTTTCGGGAATCCTTTCACACCGTTACGCGTGTTACGGAACAGCACGCGGCTGGTGCCGTGGCGATCCATCAGCATCGAAACCAGCTCCTGACGGGCGCTCTGGGCATCTTCGCTGTCGCTGTTTGCTGCCTGCAACAGCGGCTCGATA‑T‑CCT‑GCTCGCCGATCATCTCGCCGAGCATGTTCAGTTCGTCATTGCTCAGTTTGTTACCTGCCAGCAGCATGGCAACGGCGTCCGCAACCGGACGATAATTTTTCTGCTCTTCAACGAACTGCGCAAAATCGTGGAAACGGTTCGGGTCCAGCAGACGCAGACGGGCGAAGTGGCTTTCCATCCCCAGCTGTTCCGGGGTCGCGGTCAGCAGCAGAACGCCCGGCACGTGCTCTGCCAGTTGTTCAAT  >  NC_007779_cfb/61877‑62370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`