Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779_cfb	3,666,710	G→T	P314Q (CCG→CAG)	wzzE ←	Entobacterial Common Antigen (ECA)polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779_cfb	3,666,710	0	G	T	100.0%	168.4 / NA	57	P314Q (CCG→CAG)	wzzE	Entobacterial Common Antigen (ECA)polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/36);  total (21/36)

AAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCGGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATT  >  NC_007779_cfb/3666465‑3666955                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       aaaaGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGttc                                                                                                                                                                                                                                                    >  1:144488/1‑250 (MQ=255)   aaGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGttctt                                                                                                                                                                                                                                                  <  1:51422/250‑1 (MQ=255)   aaGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGGTAATGCGACACCCGCCCCGCTCAGCCCCCCCACCATGCCCC‑CCATAATCATCAGG‑AAGGCACGACCCGGGCTATCGCGCTTTTCCTGttctt                                                                                                                                                                                                                                                  >  2:51422/1‑250 (MQ=255)                          aCCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCGCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATACCACACCACCTCCCATTATCCCCCCCACAATTCGCC‑CCAAAATTATTAGG‑AAGGCAAGACGTAGGCCAGCGCGCTTTACCTGTTTTTCCGGCGTACACAAATTGC‑GATat                                                                                                                                                                                                                          >  2:939377/1‑249 (MQ=255)                                    ccATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAATACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAACCCCGATCAGCCCCCCGACAAAGCCCC‑CCCTAAACATCAGG‑AAGGCACGACCGGGGCCATCGCCGTTTACCTGTTTTTACGTCGGACGCAAATAGC‑GATAGGNCTGACAAc                                                                                                                                                                                                                >  2:468973/1‑250 (MQ=255)                                     cATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑g                                                                                                                                                                                                                              >  1:761318/1‑235 (MQ=255)                                     cATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑g                                                                                                                                                                                                                              <  2:761318/235‑1 (MQ=255)                                           ggtGGTTTACGGGCTGGGACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATc                                                                                                                                                                                                         <  2:35169/248‑1 (MQ=255)                                                 ttCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGt                                                                                                                                                                                                   <  1:501316/250‑1 (MQ=255)                                                       cgatctACCTAACACATTCAGTACTGTCACATCGATTCTCTGCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGAcc                                                                                                                                                                                             <  2:490908/245‑1 (MQ=255)                                                        cgcgTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCa                                                                                                                                                                                            <  1:257093/250‑1 (MQ=255)                                                        cgcgTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCa                                                                                                                                                                                            >  1:340712/1‑250 (MQ=255)                                                            tACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACAt                                                                                                                                                                                        <  1:292429/250‑1 (MQ=255)                                                             aCCAAACACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCCACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGGTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCCGATCCAGGGGTGGACCagatcg                                                                                                                                                                                       >  1:490908/1‑245 (MQ=255)                                                              ccAAAGACGGACAGTGTTTTCAAATCGATGCTATTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGAGCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTc                                                                                                                                                                                      <  2:267943/250‑1 (MQ=255)                                                                aaaTACAGTCAGTAATTTCAAATCGATTCTCTTCGAATAAGCGGCGGGCGCGTTTGCGCTAACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCACGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAg                                                                                                                                                                                    <  2:508229/250‑1 (MQ=255)                                                                           gtACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACa                                                                                                                                                                         >  1:668988/1‑250 (MQ=255)                                                                            ttccgatctaTCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACAt                                                                                                                                                                        <  1:471143/241‑1 (MQ=255)                                                                             aCTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGCCAATGCCCC‑CCCTAATCATCAGG‑AAGGCGCGACGTGGGCTATCGCGTTTTACCTGTTTTTCCGGCGTACCCAAATAGC‑GATAGGTCTGAAAACCCGGATCCCGGGGTGGACCAACATTCAGGGGGTTTagatcg                                                                                                                                                                       >  1:631542/1‑245 (MQ=255)                                                                                  ggcaTCGATTCTCTTCGATTAAGCGGCGTGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCTAGCAACGGCGGGATAAGGCGACACCAGCCCCGATCAGCCTCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACTACGTGAGCTATCGCGTTTTACCTATTCTTCCGGCGTACGCAAGTAGC‑GCTAGGTCTGAAAACGCGGTTCCAGGGTTGGACTCACAATCAGGGTGTTTAACATGGCCCg                                                                                                                                                                  <  2:810349/248‑1 (MQ=255)                                                                                   acaTCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAACCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGGACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCgg                                                                                                                                                                 >  1:846408/1‑249 (MQ=255)                                                                                    caTCGATTCTCTTCGAATAAGAAGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGGTAATTCGACACCATACCCCTGAAACCCCCCGACGAAGCCCCAACAGAA‑CATATGGAAAGGAACGAC‑AGGGGGAGCGCGGTTTACCGGATCTTTCTGGGTACGGAAAAAGCGGATAGGTTTGAAACCGGGAATCAAGGATT‑GACCAACCTTCCAGGGGTTTAAccatgtcccac                                                                                                                                                                >  2:106110/1‑240 (MQ=255)                                                                                     aTCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑CCATAATCCTCAGG‑AAGGCACCACGTGGGCTATCGCGTTTTACCTGTTCTTCCCGCGGACGCAAATAGC‑GATCAGTCTTGAAACGCGGATCCAGGGGTGGGCCAACAATCACGGTGTTTAACaanaatcggaa                                                                                                                                                               >  2:471143/1‑240 (MQ=255)                                                                                     aTCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGAtt                                                                                                                                                               <  1:468973/250‑1 (MQ=255)                                                                                        gATTCTCTTCGAATAAGCTGCGGGCCCCGTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTg                                                                                                                                                            <  2:846408/250‑1 (MQ=255)                                                                                              ctTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATa                                                                                                                                                      <  1:642455/250‑1 (MQ=255)                                                                                                     tAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGGGAAACCAGCCCCGATCAGCCCCCCCGCAATGCCCC‑ACATCATAAACAGG‑GAGGGAAGACCCGGGCTATTGTGCTTTAACTGCTTTTCCGGCGGACGCAAATAGA‑GAAAGGTCTGAAAACGCGATGCCAGGGTTGGATCAACATTAATGGGGTTTAAACTGGGCCGGCTCTGATccctntcncga                                                                                                                                               >  2:799417/1‑240 (MQ=255)                                                                                                                    tttGCGCTCACCGCAGCAGTTTTGCTATTTCGAGCACCGGCGGGTTTCTGCCACACCAGCCCCCATCAGCCCCCCGACAAAGCCCC‑ACCAAATCCTCAGG‑TAGGCACAGCGAGGGGTAACCCGCTTTACCTGGTCTTCCGGGGTCCGCCAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGGTTGACCCACCTTCAGGGCGTTTAAAATGGCCCGATTCTGACCATAGTCGCGGTCAAAGGCCGGAcct                                                                                                                                >  2:917021/1‑249 (MQ=255)                                                                                                                            cACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTg                                                                                                                        <  2:144488/250‑1 (MQ=255)                                                                                                                                 cagcagTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAAt                                                                                                                                                                                                                                   >  2:474463/1‑139 (MQ=255)                                                                                                                                 cagcagTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAAt                                                                                                                                                                                                                                   <  1:474463/139‑1 (MQ=255)                                                                                                                                 cagcagTGTTGCTATTTCGAGCAACGGCGGGATAATACGCCACCAGCCCACATCATCCCCCCGACAATGCCCC‑CCCTAATCATCAGG‑AAGGCGCGACTTGGCCGATCGCGCTTTTCCTGGTTTTCCGGCGCACGCAAATAGC‑GTTAGGTATGTAAAAGCGGATTCAGGGTTGGGACAACATTGAGGGTGTTTATCAAGTCCCCTTTCTGCTCACAGTCGAGATCAAAGGCCGAAACGACGGCCTGTACAt                                                                                                                   >  2:233590/1‑250 (MQ=255)                                                                                                                                      gtgtTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTcc                                                                                                              <  1:917021/250‑1 (MQ=255)                                                                                                                                        gtTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCCACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGCGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAg                                                                                                            >  1:903772/1‑250 (MQ=255)                                                                                                                                         ttGCTATTTCGAGCAACGGCGGGTTAATGCGGCACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑CCATAATCATCAGG‑AAGGCACGCCGCGGGCTATCGCGTTTTACCTGTTCTTCCGGCGCACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGGGTTTAACATGGCCCGATTCTGATCATAGTCCAGATTAAAGGCCGGACCGACGGCCTGTAAATTTTCCAgc                                                                                                           >  2:164142/1‑249 (MQ=255)                                                                                                                                                    ggCAACGGCGTGGTAAGGCGCAGCCAGGCACGGTCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGAGGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAg                                                                                                <  2:677823/249‑1 (MQ=255)                                                                                                                                                          ggcggGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTgg                                                                                          <  1:799417/250‑1 (MQ=255)                                                                                                                                                                ttAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGAcc                                                                                                                                                                                             <  1:858962/145‑1 (MQ=255)                                                                                                                                                                ttAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGAcc                                                                                                                                                                                             >  2:858962/1‑145 (MQ=255)                                                                                                                                                                       gACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCACAg                                                                                                                                                     <  1:887491/178‑1 (MQ=255)                                                                                                                                                                       gACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCACAg                                                                                                                                                     >  2:887491/1‑178 (MQ=255)                                                                                                                                                                             aGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCgag                                                                                                                                                >  1:600741/1‑177 (MQ=255)                                                                                                                                                                             aGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCgag                                                                                                                                                <  2:600741/177‑1 (MQ=255)                                                                                                                                                                             aGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACAtt                                                                       >  2:287395/1‑250 (MQ=255)                                                                                                                                                                             aGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGCTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCa                                                                                                             >  1:682852/1‑212 (MQ=255)                                                                                                                                                                             aGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGCTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCa                                                                                                             <  2:682852/212‑1 (MQ=255)                                                                                                                                                                                                 cAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTc                                                   <  1:287395/250‑1 (MQ=255)                                                                                                                                                                                                                atcatcAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCATGTAATTCCTCGGCAGGTACATCTg                                     <  1:255900/250‑1 (MQ=255)                                                                                                                                                                                                                 tcatcaGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGa                                                                                                                                                           <  1:389967/131‑1 (MQ=255)                                                                                                                                                                                                                 tcatcaGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGa                                                                                                                                                           >  2:389967/1‑131 (MQ=255)                                                                                                                                                                                                                 tcatcaGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAGTTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGt                                    <  1:321608/250‑1 (MQ=255)                                                                                                                                                                                                                  catcaGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTc                                   <  1:164142/250‑1 (MQ=255)                                                                                                                                                                                                                    tcaGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGc                                 >  1:519521/1‑250 (MQ=255)                                                                                                                                                                                                                     caGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGCTCATAGTCGAGATCAAAGGCCGTACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCa                                >  1:655090/1‑250 (MQ=255)                                                                                                                                                                                                                           cggCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTCAATCTGTTGCAccggg                           >  2:702013/2‑246 (MQ=255)                                                                                                                                                                                                                           aGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTgcg                           <  1:564644/250‑1 (MQ=255)                                                                                                                                                                                                                           aGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTgcg                           <  2:668988/250‑1 (MQ=255)                                                                                                                                                                                                                           aGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTgc                            <  2:203288/249‑1 (MQ=255)                                                                                                                                                                                                                           aGGCACGACGTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTgc                            >  1:203288/1‑249 (MQ=255)                                                                                                                                                                                                                               acgacgTGGGCTATCGCTTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTGCGCGaa                       <  2:903772/250‑1 (MQ=255)                                                                                                                                                                                                                                 gacgTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTGCGCGAAat                     <  1:702013/250‑1 (MQ=255)                                                                                                                                                                                                                                   cgTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCt                                                                                                                         <  1:366556/148‑1 (MQ=255)                                                                                                                                                                                                                                   cgTGGGCTATCGCGTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCt                                                                                                                         >  2:366556/1‑148 (MQ=255)                                                                                                                                                                                                                                            gtgcgTTTTACCTGTTCTTCCGGCGTACGCAAATAGC‑GATGGGTCTGAAAACGCGGATCCAAGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTGCGCGAAATATTATgctgct          <  2:519521/248‑1 (MQ=255)                                                                                                                                                                                                                                                    tACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAGGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAAtt  <  1:233590/250‑1 (MQ=255)                                                                                                                                                                                                                                                    tACCTGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAAtt  <  2:340712/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       AAAAGGATCTTTTGCCAACGCATGCACCAACGGCGCCATCTTGATGGCTTCCGGGCGCGTACCAAATACAGTCAGTACTTTCACATCGATTCTCTTCGAATAAGCGGCGAGCGCCTTTGCGCTCACCGCAGCAGTGTTGCTATTTCGAGCAACGGCGGGTTAATGCGACACCAGCCCCGATCAGCCCCCCGACAATGCCCC‑ACATAATCATCAGG‑AAGGCACGACGTGGGCTATCGCGTTTTACCGGTTCTTCCGGCGTACGCAAATAGC‑GATAGGTCTGAAAACGCGGATCCAGGGTTGGACCAACATTCAGGGTGTTTAACATGGCCCGATTCTGATCATAGTCGAGATCAAAGGCCGGACCGACGGCCTGTAAATTTTCCAGTCGAGCCTGAAGCATTGGACGCCCAAGCAGGAACATTTCTGAATCAGGTAATTCCTCGGCAGGTACATCTGTCGCACTGCGCGAAATATTATGCTGCTCAGCAATT  >  NC_007779_cfb/3666465‑3666955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑