BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779_cfb	2,245,455	A→G	S283P (TCG→CCG)	yeiB ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779_cfb	2,245,455	0	A	G	99.0%	276.0 / ‑6.2	103	S283P (TCG→CCG)	yeiB	inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (49/53); minor base C (1/0); total (50/53)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 108 total aligned reads displayed.

TGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAG  >  NC_007779_cfb/2245220‑2245689

tGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGCATAAATCCGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCg                                                                                                                                                                                                                              >  1:508258/1‑250 (MQ=255)

         tAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACg                                                                                                                                                                                                                     <  1:1177425/250‑1 (MQ=255)

                    gATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGCGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGt                                                                                                                                                                                                          >  1:672287/1‑250 (MQ=255)

                      tAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACACACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTTCGTCGCAGGCGATCGCAAGCACCAGCTTAAAGCAGCTCAATTGCCGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAAGCGCCTGCAACGGCGCACTCAGGTc                                                                                                                                                                                                        >  2:1274450/1‑250 (MQ=255)

                       aaCAGAGAAGGGGATACTCCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTAAAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCGCCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTcc                                                                                                                                                                                                       <  2:641808/250‑1 (MQ=255)

                       aaCAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAACTGCATAAACAAACCGAGGTGGTAAAAAAGCGCGGGACAAATCAGCGTTTGCACTACATAGTTGGTCAGCGCCATCCGTCCGCCGCAGGCGATCGCAACCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AACACAGCGGCGCATAGCCAATCGCCTGAAACCGAGCACTCCGTTcc                                                                                                                                                                                                       >  2:350620/1‑250 (MQ=255)

                            ggaggagGAAATCAGCAAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTAAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAAGAGATAGTTGGTCAGCGCCATCAGTCCGACGCAGGCGAGCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGGGCATAGCCGATCGCCTGAAACGGCGCACTCAGTTCCCGCgg                                                                                                                                                                                                  <  2:508258/246‑1 (MQ=255)

                             gaagaGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGc                                                                                                                                                                                                 >  2:160536/1‑250 (MQ=255)

                               agagGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCAt                                                                                                                                                                                               >  2:171054/1‑250 (MQ=255)

                                 aganttncccccagccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCAttt                                                                                                                                                                                             <  2:1185317/240‑1 (MQ=255)

                                 agGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATa                                                                                                                                                                                                                                      <  2:163341/209‑1 (MQ=255)

                                 agGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATa                                                                                                                                                                                                                                      >  1:163341/1‑209 (MQ=255)

                                      atTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGt                                                                                                                                                                                        <  1:160536/250‑1 (MQ=255)

                                            cagccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTcc                                                                                                                                                                                                       <  1:994621/229‑1 (MQ=255)

                                                  taacGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGaa                                                                                                                                                                                          >  2:149417/4‑236 (MQ=255)

                                                  tACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGTCGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGaa                                                                                                                                                                                          <  1:149417/236‑1 (MQ=255)

                                                        aaTAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGa                                                                                                                                                                      >  1:349008/1‑250 (MQ=255)

                                                           aaCAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATAc                                                                                                                                                                   >  1:869878/1‑250 (MQ=255)

                                                                ccGCAAGCAGCTCCAGGCGGTCAAAATGAATAAACACACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGAAATAGATAGTTGGCCAGCGCCTTCCGTCCGACGCAGGCGATCGCAAGCACAAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACGGCGGCGCAGAGTCAATCGCCTGAAACGGCGCACTCAGTACCCGCGGCATTTGAAGTTAGAGGGCACACGAGCGATACGCCCa                                                                                                                                                              <  2:642216/249‑1 (MQ=255)

                                                                     agcagcTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATAc                                                                                                                                                                   <  2:794232/240‑1 (MQ=255)

                                                                     agcagcTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGCAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATAc                                                                                                                                                                   >  1:794232/1‑240 (MQ=255)

                                                                       cagcTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTGAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGc                                                                                                                                                       >  2:708805/1‑250 (MQ=255)

                                                                          cTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGCTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCATGAAACGGCGCCCTCGGTTCCCGAGGCATTTGAAGTAAGAAGGAACAACGGCGGAAAGCCAAGTCCacaggc                                                                                                                                                    >  2:14964/1‑245 (MQ=255)

                                                                              aGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCAtt                                                                                                                                                >  1:1351023/1‑250 (MQ=255)

                                                                               ggCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGTAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTcc                                                                                                                                                          <  2:914213/239‑1 (MQ=255)

                                                                               ggCGGTCAAAATGCATAAACAAACCGAGGGGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTcc                                                                                                                                                          >  1:914213/1‑239 (MQ=255)

                                                                                         aTGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCGAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCGGGGCGATg                                                                                                                                     >  1:465531/1‑250 (MQ=255)

                                                                                         aTGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATg                                                                                                                                     >  2:172944/1‑250 (MQ=255)

                                                                                                aacaaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAGGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGaa                                                                                                                              >  1:1000854/1‑250 (MQ=255)

                                                                                                  caaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAg                                                                                                                                                             >  1:922299/1‑217 (MQ=255)

                                                                                                  caaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAg                                                                                                                                                             <  2:922299/217‑1 (MQ=255)

                                                                                                  caaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAg                                                                                                                                            >  2:132830/1‑234 (MQ=255)

                                                                                                  caaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAg                                                                                                                                            <  1:132830/234‑1 (MQ=255)

                                                                                                  caaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTGGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGGTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAgg                                                                                                                            <  1:14964/250‑1 (MQ=255)

                                                                                                   aaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCa                                                                                                                                                                          <  2:697887/203‑1 (MQ=255)

                                                                                                   aaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCa                                                                                                                                                                          >  1:697887/1‑203 (MQ=255)

                                                                                                    aacCGAGGTGGTAAAAAAGCGCGGTACAAATCAGCGTTTGGAATAGATAGTTGGTCAGCGCCATCCGTCCGAAGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt                                                                                                                          >  2:360307/1‑250 (MQ=255)

                                                                                                     acCGAGTAGGTAAAAAAGCGTGGTAAAAATCAGCGGTTGCAATAGATAGTGGGTCAGCGCCAGCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCAAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTa                                                                                                                         <  2:244393/250‑1 (MQ=255)

                                                                                                       cGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAggg                                                                                                                                          <  1:1017697/231‑1 (MQ=255)

                                                                                                       cGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAggg                                                                                                                                          >  2:1017697/1‑231 (MQ=255)

                                                                                                               tAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGca                                                                                                                                                                               >  1:753109/1‑186 (MQ=255)

                                                                                                               tAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGca                                                                                                                                                                               <  2:753109/186‑1 (MQ=255)

                                                                                                                aaaaagGCGTGGTCCAACTCAGCGTTTGCAATAGATATTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGTCCAGAAGCCAT‑AAAACATCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCTGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCAcccc                                                                                                              <  2:869878/250‑1 (MQ=255)

                                                                                                                 aaaaaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGa                                                                                                                               >  1:217051/1‑232 (MQ=255)

                                                                                                                 aaaaaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGa                                                                                                                               <  2:217051/232‑1 (MQ=255)

                                                                                                                 aaaaaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCa                                                                                                             <  1:171054/250‑1 (MQ=255)

                                                                                                                   aaaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAAt                                                                                                           <  1:1178169/250‑1 (MQ=255)

                                                                                                                   aaaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAAt                                                                                                           <  1:172944/250‑1 (MQ=255)

                                                                                                                      gCGTGGTAGAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATCTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGc                                                                                                        >  1:771386/1‑250 (MQ=255)

                                                                                                                            tACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCGTTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAg                                                                                                  >  1:534101/1‑250 (MQ=255)

                                                                                                                            tACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAgg                                                                                                                            <  1:103831/224‑1 (MQ=255)

                                                                                                                            tACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGCAAgg                                                                                                                            >  2:103831/1‑224 (MQ=255)

                                                                                                                             aCAAATTAGCGTCTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCa                                                                                                                                                                                                                <  1:890395/139‑1 (MQ=255)

                                                                                                                             aCAAATTAGCGTCTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCa                                                                                                                                                                                                                >  2:890395/1‑139 (MQ=255)

                                                                                                                                 aTCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGCCGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGCAGCCTT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTCGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCACCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACaa                                                                                             >  1:1081709/1‑250 (MQ=255)

                                                                                                                                   cAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCacac                                                                                                                                                                            <  1:618249/169‑1 (MQ=255)

                                                                                                                                   cAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCacac                                                                                                                                                                            >  2:618249/1‑169 (MQ=255)

                                                                                                                                           gCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAAt                                                                                                           <  1:636854/226‑1 (MQ=255)

                                                                                                                                           gCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAAt                                                                                                           >  2:636854/1‑226 (MQ=255)

                                                                                                                                            cAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGCTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTacga                                                                                  >  1:1269856/1‑250 (MQ=255)

                                                                                                                                             aaTAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTacgac                                                                                 <  1:350620/250‑1 (MQ=255)

                                                                                                                                             aaTAGATAGTTGGTCAGCCCCATCCGTCCGACGCATGCGATCGCAAGCACCAGCTTAACGCGGCTCACTTGCGCCCAGAAGCCAT‑AAAACAGCGGCGCATATCCAATCGCCTGAAACGGCGCACTCCGTTCCCGCGGCATTTGATGTAAGCAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGCCGATGGCAGGACGCGTAAGGGTCCCCCCCATCGCTCCCTCCACAACACCCGTaccac                                                                                 >  2:1341500/1‑250 (MQ=255)

                                                                                                                                              atagatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCg                                                                                                         <  2:969739/225‑1 (MQ=255)

                                                                                                                                              atagatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTacgacg                                                                                >  2:158783/1‑250 (MQ=255)

                                                                                                                                               tagctgggtGGGCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCGTTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTAcgacga                                                                               <  2:534101/242‑1 (MQ=255)

                                                                                                                                               tagatagGTGGTCAGCGCCACCCGTCCGAGGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAAGTGCGGCCAGAAGCCATAAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCAAAAAACCAGTacgacg                                                                                <  2:1205991/250‑1 (MQ=255)

                                                                                                                                                   tagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGt                                                                           >  2:624319/1‑250 (MQ=255)

                                                                                                                                                    cgTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATCGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTg                                                                          >  2:1076808/2‑250 (MQ=255)

                                                                                                                                                     gTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGa                                                                         <  1:708805/250‑1 (MQ=255)

                                                                                                                                                     gTTGGTCAGCGCCAGCCGTCCGACGCAGGCGATCGCAAGGACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCAGAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCTCCAGCACAAAACCAGTACGACGATAGTGa                                                                         <  1:180791/250‑1 (MQ=255)

                                                                                                                                                      ttGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCGCCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATg                                                                                                                      <  1:846534/204‑1 (MQ=255)

                                                                                                                                                      ttGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCGCCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATg                                                                                                                      >  2:846534/1‑204 (MQ=255)

                                                                                                                                                          tCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTaa                                                                    <  1:624319/250‑1 (MQ=255)

                                                                                                                                                           cAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAg                                                                   >  1:320781/1‑250 (MQ=255)

                                                                                                                                                              cgcCATCCGGCCGACGCAGGCGATCGCAAGCCCCAGCTTAAAGAGGCTCAATGGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTg                                                                <  2:320781/250‑1 (MQ=255)

                                                                                                                                                                          gACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATCGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTc                                                    <  1:1076808/250‑1 (MQ=255)

                                                                                                                                                                             gCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCa                                                                                        <  2:435774/211‑1 (MQ=255)

                                                                                                                                                                             gCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCa                                                                                        >  1:435774/1‑211 (MQ=255)

                                                                                                                                                                                 gCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTTCCCTTTcagccacc                                             >  1:468863/1‑248 (MQ=255)

                                                                                                                                                                                  cGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGCCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCagccagcc                                            >  1:10209/1‑250 (MQ=255)

                                                                                                                                                                                    aTCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATa                                                                             >  1:264717/1‑215 (MQ=255)

                                                                                                                                                                                    aTCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATa                                                                             <  2:264717/215‑1 (MQ=255)

                                                                                                                                                                                     tCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAATCAGCGGCGCATAGCCAATCGCCTGAAATGGCGCACCCCATTCCCGCGGCATTTGAAGTAAGCAGGCACACCAGCGATCCGCCCAGTACAGCTGCCATTGCAGGGCGATGGCAGGCAGGTTAATGGTCACCCCCATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGATGAACTGCCCTTTCAGCCngcngcc                                         >  2:1144556/1‑249 (MQ=255)

                                                                                                                                                                                       gctaGCGCCAGCTGAAATCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgc                                       <  2:1351023/247‑1 (MQ=255)

                                                                                                                                                                                        cAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGc                                                          <  2:525025/230‑1 (MQ=255)

                                                                                                                                                                                        cAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGc                                                          >  1:525025/1‑230 (MQ=255)

                                                                                                                                                                                        aaaGCTCTAGCTTAAGTCGGCTCAATTGCGGCCCGGAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACACAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCCCCAGCACAAAACCAGTACGGCGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgcg                                      <  2:10209/249‑1 (MQ=255)

                                                                                                                                                                                           gCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCAt                                   >  2:509590/1‑250 (MQ=255)

                                                                                                                                                                                                aGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAgcg                              <  1:360307/250‑1 (MQ=255)

                                                                                                                                                                                                 gCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAAGCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAgcgc                             <  2:326106/250‑1 (MQ=255)

                                                                                                                                                                                                     aaaGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCATCCGCTGCGCATCAGCGCCGCa                         <  1:1341500/250‑1 (MQ=255)

                                                                                                                                                                                                          ggCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAAt                    <  1:158783/250‑1 (MQ=255)

                                                                                                                                                                                                             tCAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAg                 <  1:509590/250‑1 (MQ=255)

                                                                                                                                                                                                              cAATTGCGGCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGc                <  1:1402909/250‑1 (MQ=255)

                                                                                                                                                                                                                     ggCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCTTGAAACGGCGCACTAAGTTACCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACTCACAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCTCTTTCAGCCAGCCGCTGCGCATCAGCTCCGCACCAATGANCATAATcc         >  2:727468/1‑250 (MQ=255)

                                                                                                                                                                                                                     ggCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAAc                                                                                          >  1:559746/1‑169 (MQ=255)

                                                                                                                                                                                                                     ggCCAGAAGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAAc                                                                                          <  2:559746/169‑1 (MQ=255)

                                                                                                                                                                                                                            aGCCAT‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATCTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGACAg  <  2:771386/250‑1 (MQ=255)

                                                                                                                                                                                                                                    aaaCAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGcaca                                                                                              >  2:1018138/1‑151 (MQ=255)

                                                                                                                                                                                                                                    aaaCAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGcaca                                                                                              <  1:1018138/151‑1 (MQ=255)

TGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCAT‑AAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAG  >  NC_007779_cfb/2245220‑2245689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`