Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779_cfb 4,371,274 (A)5→3 intergenic (‑9/+296) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779_cfb4,371,2710A.83.3% 86.8 / 22.2 36intergenic (‑6/+300)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/6);  new base . (27/3);  total (27/9)
Fisher's exact test for biased strand distribution p-value = 4.31e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_007779_cfb4,371,2720A.83.3% 90.0 / 19.0 36intergenic (‑7/+299)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/6);  new base . (27/3);  total (27/9)
Fisher's exact test for biased strand distribution p-value = 4.31e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTA  >  NC_007779_cfb/4371022‑4371507
                                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                           
aTGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTTTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAGa                                                                                                                                                                                                                                              >  1:285876/1‑250 (MQ=255)
   cGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCGGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GATCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                                                                                                                                                                                                                                           <  2:213423/250‑2 (MQ=255)
   cGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                                                                                                                                                                                                                                           >  2:361693/1‑249 (MQ=255)
       gATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAGaaatagcg                                                                                                                                                                                                                                       <  1:361693/250‑6 (MQ=255)
            cAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                                                                                                                                                                                                                >  1:409471/1‑250 (MQ=255)
             aaTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTATCGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                                                                                                                                                                                                               >  1:145134/1‑250 (MQ=255)
                cTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTATCGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                                                                                                                                                                                                                            <  2:145134/250‑1 (MQ=255)
                 tGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCg                                                                                                                                                                                                                           <  2:90044/250‑1 (MQ=255)
                 tGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCg                                                                                                                                                                                                                           >  1:403400/1‑250 (MQ=255)
                             aCTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATgg                                                                                                                                                                                                               >  1:1309702/1‑250 (MQ=255)
                              cTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga                                                                                                                                                                                                              >  1:520533/1‑250 (MQ=255)
                                    tagagcgCGGAGGGCAGGTTTTAACTCGTTGTTCCTCCCAAACTTCAGCGAAAATTGGCGGGGCCGCAGAAGAGAGGTTGCCATTACAAGAAAAGATAGTCAGAAAATA‑GGTGCCGATGGGTGCGGGG‑TTGGTGATGTATTGGGGG‑TTACGGCGAACCAGCTTTTCTGTCTGAGGAACA‑AGA‑TAGTCCAGCCCGCCAGAAACCTGCAGGGC‑‑AAAAATAGCGGCGATAACCGCCATGATAATGGAGATGAc                                                                                                                                                                                                        <  2:1309702/244‑1 (MQ=255)
                                         aGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGa                                                                                                                                                                                                   >  1:798202/1‑250 (MQ=255)
                                             gACGGCAAGGATTAACGCCTTGTTCCTTCGCAACTTCAGCGATAATTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACgg                                                                                                                                                                                               >  1:1027617/1‑250 (MQ=255)
                                                 tcAAGTTTTAACGCTTTTTCCCTTCGCATCTACAGAGAAAACGGGGAGGGCCCCGAGAGATATGTTGCCACTCAGAAAAAAGATAGTGAGAAAAAA‑GGTCAGGTGGGGTGAGGGG‑ATCGGGATTTATTGCGGT‑TAGCGGCGGAAAAGTTTTCCTGCTTGATGAGCC‑TGA‑TCGTCGAGACCGCCAAAACCCTGCA‑GGGA‑AAAAATGGCGCCGATAGCCGCCATGATAATGGAGATGACATCGAACGGGATg                                                                                                                                                                                           <  2:79485/249‑1 (MQ=255)
                                                           aCGCTTTGTTCTTTCGAAACTTCGAGGATAACTGGCAGTTGCGAAGGGGAGATGTCGCCAGTCCCACAAAAGATA‑TCCAGAAATAGGGCCACGGTCGGGGGGAGG‑ATGGTATTGT‑TTTCGGGTTTCCGGCCCAGCGGTTTTTCTTTCTAAT‑AACC‑AAATTACTCCCGCCCGCCGGAACCCTGCA‑GGGA‑AAAAATGGGGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt                                                                                                                                                                                 <  2:613179/250‑1 (MQ=255)
                                                                   ttCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                                                                                                                                                                                                               <  2:640272/196‑1 (MQ=255)
                                                                   ttCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                                                                                                                                                                                                               >  1:640272/1‑196 (MQ=255)
                                                                       nccacNACTACAGCGATAGCTGGACGTGCCGCCAGGAAAATGTTGCAAGTACCAGTAAAGATAGTAGGAAAATG‑GGGCGGGATGGGGGCGAGG‑GTGGTAATGTTTTTGGGT‑TTAGGGCGCGGCGTTTTTTCTGTCTGATGACCC‑AGA‑TAGTCAAGCCCGCCACCAACCGGCTTGG‑A‑AAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAg                                                                                                                                                                     <  2:798202/246‑1 (MQ=255)
                                                                                        aaCTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAcc                                                                                                                                                    >  1:898729/1‑250 (MQ=255)
                                                                                        aaCTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAcc                                                                                                                                                    >  1:577257/1‑250 (MQ=255)
                                                                                           tGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAccccc                                                                                                                                                 >  1:1175630/1‑250 (MQ=255)
                                                                                           tGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAccccc                                                                                                                                                 >  1:3224/1‑250 (MQ=255)
                                                                                            ggCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCa                                                                                                                                                >  1:981252/1‑250 (MQ=255)
                                                                                                    cggggaggagaTGTGCCCAGCACAAGAAAAGATAG‑AAAGAAATG‑GGTCACGGGGGGGGGGGGGCTTGGTGTTGTTTTTGGGT‑TTCCGGCACAGCAGCTTTTTTTTCGGA‑GAACCAAGA‑AAGCCCAGCCCGCCAGCACCCTGCATGGC‑‑AAAAATAGCGGGAATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCt                                                                                                                                        <  2:159791/243‑1 (MQ=255)
                                                                                                                    ccAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTa                                                                                                                        >  2:442842/1‑250 (MQ=255)
                                                                                                                        tACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCATACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTcc                                                                                                                    >  1:475268/1‑250 (MQ=255)
                                                                                                                                        cAGGAAATA‑GGTCACGATCAGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGccc                                                                                                    >  1:1308912/1‑250 (MQ=255)
                                                                                                                                          ggAAATA‑GGTCGCGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCACTCTGGCGCCCaa                                                                                                  >  1:798728/1‑250 (MQ=255)
                                                                                                                                              aTA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCaagaag                                                                                              >  1:554007/1‑250 (MQ=255)
                                                                                                                                                         aTCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCa                                                                                    >  1:380952/1‑250 (MQ=255)
                                                                                                                                                          tCGGTTCTAGG‑ACCGTGATGTATTTCGGG‑TTACGGCGCGGCCGCTTTTCTGTCTGATGAACC‑AGA‑TCGTCCAGACCGCCAGCAACCTGCCTGGCAG‑‑AAATAGAGGCGATAACCGCCATGATAATGGAGTTTACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGAGGCAAGAACACGCAACCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGAACGNCANGaa                                                                                   >  2:1095835/1‑250 (MQ=255)
                                                                                                                                                              tGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACt                                                                               >  1:469321/1‑250 (MQ=255)
                                                                                                                                                                cGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCG‑CATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTatg                                                                            >  1:540425/1‑250 (MQ=255)
                                                                                                                                                                                         ttACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCTAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATAATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCatat                                                      >  1:203655/1‑250 (MQ=255)
                                                                                                                                                                                             ggCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGc                                                  >  1:1306540/1‑250 (MQ=255)
                                                                                                                                                                                               cgcAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGcct                                                >  1:916220/1‑250 (MQ=255)
                                                                                                                                                                                                          ttCTGTCTGATGAACC‑AGA‑TAGCCCAGCCCGCCAGCACCCTGCATGGC‑‑AAAAATAGGGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGttttt                                     <  1:510120/250‑1 (MQ=255)
                                                                                                                                                                                                              gtctgATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATAATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAAc                                 >  2:99919/1‑250 (MQ=255)
                                                                                                                                                                                                                                               cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTa  >  1:404155/1‑250 (MQ=255)
                                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                           
ATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAATA‑GGTCACGATCGGTGCGAGG‑ATCGTGATGTATTTCGGG‑TTACGGCGCAGCAGCTTTTCTGTCTGATGAACC‑AGA‑TAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTA  >  NC_007779_cfb/4371022‑4371507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑