breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 105,411 G→A G36D (GGT→GAT)  ftsZ → cell division protein FtsZ
RA 444,525 T→G intergenic (+127/+1) yajQ → / ← Y75_RS02195 nucleotide binding protein YajQ/MFS transporter
RA 490,998 Δ1 bp coding (363/552 nt) apt → adenine phosphoribosyltransferase
RA 520,989 G→T P450P (CCG→CCT Y75_RS02550 → ABC transporter permease
RA 547,694 A→G E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,835 +G coding (255/261 nt) Y75_RS02655 → hypothetical protein
RA 556,778 A→C F63V (TTC→GTC)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
MC JC 654,214 Δ1,199 bp Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,093,686 T→C V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
MC JC 1,301,041 Δ1,336 bp Y75_RS06530Y75_RS06530 Y75_RS06530, Y75_RS06530
RA 1,519,222 G→T V40V (GTG→GTT Y75_RS07640 → orphan toxin OrtT
RA 1,565,001 A→G intergenic (‑211/+47) ddpX ← / ← dosP D‑alanyl‑D‑alanine dipeptidase/oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
RA 1,669,601 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,882,210 G→A L88L (CTC→CTT leuE ← leucine efflux protein LeuE
RA 1,932,183 Δ1 bp coding (279/291 nt) yebG ← DNA damage‑inducible protein YebG
RA 1,979,957 G→T intergenic (‑46/+398) flhD ← / ← Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← H48 family flagellin FliC
MC JC 2,176,983 Δ1,199 bp Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,245,455 A→G S283P (TCG→CCG)  Y75_RS11290 ← DUF418 family protein
MC JC 2,320,795 Δ1,336 bp Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,434,645 A→T V347E (GTG→GAG)  purF ← amidophosphoribosyltransferase
RA 2,866,111 A→G pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
MC JC 3,261,134 Δ1,199 bp Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  Y75_RS16240 → YhaC family protein
JC 3,277,128 (TTCAACA)2→3 coding (272/336 nt) prlF → type II toxin‑antitoxin system antitoxin PrlF
MC JC 3,742,936 Δ1,336 bp Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) Y75_RS19595 → C4‑dicarboxylate transporter DctC
MC JC 4,310,363 Δ1,199 bp Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1197780 1213137 15358 134 [133] [131] 134 [icd]–[Y75_RS23470] [icd],Y75_RS05945,Y75_RS05950,lit,Y75_RS05960,Y75_RS05965,Y75_RS24820,Y75_RS23460,Y75_RS05970,Y75_RS05975,Y75_RS05980,ymfL,Y75_RS23465,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,Y75_RS06015,Y75_RS06020,Y75_RS06025,Y75_RS06030,Y75_RS06035,pinE,Y75_RS06045,[Y75_RS23470]
* * ÷ NC_007779 3974162 3988254–3987179 13018–14093 176 [0] [133] 134 [Y75_RS19655]–Y75_RS19725 [Y75_RS19655],gadX,gadW,Y75_RS19670,mdtE,Y75_RS19680,gadE,hdeD,hdeA,hdeB,Y75_RS19705,Y75_RS19710,slp,Y75_RS19720,Y75_RS19725

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 273179 =NA (NA)214 (1.010) 169/496 0.2 99.1% intergenic (+2/+146) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
?NC_007779 1106950 = 2 (0.010)intergenic (‑44/‑41) Y75_RS05455/Y75_RS05465 IS3‑like element IS2 family transposase/hypothetical protein
* ? NC_007779 = 274373NA (NA)234 (1.100) 184/496 0.1 99.6% intergenic (‑32/‑176) Y75_RS01335/mmuP IS5‑like element IS5 family transposase/S‑methylmethionine permease
?NC_007779 = 380548 1 (0.000)coding (19/361 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 380484 =NA (NA)194 (0.910) 155/496 0.5 NA coding (28/444 nt) Y75_RS01845 transferase
?NC_007779 = 381407 NA (NA)coding (518/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 = 2072408NA (NA)177 (0.840) 144/492 0.9 NA intergenic (‑46/‑3) Y75_RS10480/Y75_RS10490 IS3‑like element IS2 family transposase/TonB‑dependent receptor plug domain‑containing protein
?NC_007779 = 2105081 NA (NA)intergenic (‑33/+2) Y75_RS10660/Y75_RS10665 IS5‑like element IS5 family transposase/glycosyltransferase
* ? NC_007779 2292253 =NA (NA)226 (1.060) 174/496 0.2 97.4% pseudogene (3/2532 nt) yejO autotransporter YejO
?NC_007779 = 2487193 6 (0.030)coding (430/1539 nt) emrY multidrug efflux MFS transporter permease subunit EmrY
* ? NC_007779 = 2293447NA (NA)147 (0.690) 120/496 2.3 95.5% intergenic (‑32/‑387) Y75_RS11495/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
?NC_007779 2487190 = 7 (0.030)coding (433/1539 nt) emrY multidrug efflux MFS transporter permease subunit EmrY
* ? NC_007779 = 3186081NA (NA)226 (1.070) 183/494 0.1 98.7% coding (26/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3512753 = 3 (0.010)coding (529/1026 nt) cytR DNA‑binding transcriptional regulator CytR
* ? NC_007779 = 35127595 (0.020)255 (1.200) 180/494 0.1 98.1% coding (535/1026 nt) cytR DNA‑binding transcriptional regulator CytR
?NC_007779 = 3744262 NA (NA)intergenic (‑45/+31) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 = 3744202NA (NA)220 (1.040) 173/496 0.2 NA coding (16/361 nt) Y75_RS18560 IS3‑like element IS2 family transposase
?NC_007779 = 3748105 NA (NA)intergenic (+146/+3) Y75_RS18585/tnaB IS5‑like element IS5 family transposase/low affinity tryptophan permease TnaB
* ? NC_007779 = 3744263NA (NA)194 (0.910) 159/496 0.4 99.0% intergenic (‑46/+30) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
?NC_007779 3903274 = 2 (0.010)coding (37/825 nt) Y75_RS19350 protein bax
* ? NC_007779 3746912 =NA (NA)176 (0.830) 141/494 1.0 100% intergenic (‑4/‑31) Y75_RS18580/Y75_RS18585 tryptophan permease/IS5‑like element IS5 family transposase
?NC_007779 = 3974161 0 (0.000)coding (1327/1401 nt) Y75_RS19655 glutamate decarboxylase
* ? NC_007779 = 37505900 (0.000)263 (1.260) 192/488 0.0 100% intergenic (‑104/‑33) tnaA/Y75_RS18600 tryptophanase/IS5‑like element IS5 family transposase
?NC_007779 3751790 = 0 (0.000)intergenic (+151/+116) Y75_RS18600/tnaC IS5‑like element IS5 family transposase/tryptophanase leader peptide
* ? NC_007779 = 39032794 (0.020)255 (1.200) 191/494 0.0 98.5% coding (42/825 nt) Y75_RS19350 protein bax
?NC_007779 = 4504190 NA (NA)intergenic (+10/+167) Y75_RS22280/Y75_RS22285 IS3‑like element IS2 family transposase/phosphoethanolamine transferase
* ? NC_007779 = 3988377NA (NA)214 (1.020) 164/492 0.3 99.5% coding (748/783 nt) Y75_RS19730 hypothetical protein
?NC_007779 = 4547133 1 (0.000)coding (417/597 nt) fimE type 1 fimbria regulatory protein FimE
* ? NC_007779 = 3988379NA (NA)271 (1.270) 188/496 0.0 98.6% coding (746/783 nt) Y75_RS19730 hypothetical protein
?NC_007779 4495464 = 4 (0.020)coding (356/999 nt) idnR DNA‑binding transcriptional regulator IdnR
* ? NC_007779 4218985 =2 (0.010)304 (1.440) 184/494 0.1 99.4% intergenic (+186/‑83) metA/aceB homoserine O‑succinyltransferase/malate synthase A
?NC_007779 4310364 = NA (NA)intergenic (‑288/‑31) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
* ? NC_007779 = 42189897 (0.030)314 (1.480) 183/496 0.1 97.8% intergenic (+190/‑79) metA/aceB homoserine O‑succinyltransferase/malate synthase A
?NC_007779 = 4311557 NA (NA)pseudogene (921/921 nt) alsK allose kinase
* ? NC_007779 4310363 =NA (NA)256 (1.200) 189/496 0.0 96.6% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4495467 9 (0.040)coding (353/999 nt) idnR DNA‑binding transcriptional regulator IdnR
* ? NC_007779 4310363 =NA (NA)195 (0.920) 155/496 0.5 96.5% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4547128 = 7 (0.030)coding (412/597 nt) fimE type 1 fimbria regulatory protein FimE
* ? NC_007779 = 4311556NA (NA)193 (0.910) 154/494 0.5 96.0% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 4540915 = 8 (0.040)intergenic (+204/+379) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 4547362 =55 (0.260)170 (0.830) 141/478 0.8 74.4% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 4547676 = 64 (0.310)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 = 454737055 (0.260)173 (0.840) 145/478 0.7 74.7% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 = 4547666 64 (0.310)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA