breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 69 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,097,5800CT32.4% 127.9 / 93.7 105intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077793,895,0180TG29.4% 94.0 / 13.9 68L198F (TTA→TTCyiaN2,3‑diketo‑L‑gulonate transporter large permease YiaN
*NC_007779753,5470TA28.6% 383.2 / 66.5 168intergenic (‑330/+60)Y75_RS03720/gltAtype 1 fimbrial protein/citrate (Si)‑synthase
*NC_0077793,205,0910CT28.5% 274.8 / 54.2 144intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077792,004,0550AC27.8% 352.7 / 29.5 151intergenic (‑129/+192)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_0077794,108,9760TG26.9% 531.6 / 19.8 217Y334D (TAT→GAT) Y75_RS20260DUF4153 domain‑containing protein
*NC_0077794,609,0240CT26.8% 287.9 / 44.6 142S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NC_0077792,902,3480CA26.5% 438.4 / 67.4 167intergenic (+318/+1055)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_007779432,8580TG26.3% 264.0 / 19.7 153G60G (GGT→GGGribDbifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD
*NC_0077791,437,4410GA26.1% 301.2 / 58.3 134intergenic (‑108/+33)uspF/ompNuniversal stress protein UspF/porin OmpN
*NC_0077791,826,7750GA26.1% 336.8 / 44.4 142intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
*NC_0077794,291,3090TA25.4% 401.5 / 68.2 173intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_0077793,535,1740AC25.2% 375.7 / 14.6 159intergenic (‑183/+77)kdgT/sodA2‑keto‑3‑deoxygluconate transporter/superoxide dismutase [Mn]
*NC_0077794,585,3140CT25.0% 400.2 / 37.0 164E277K (GAA→AAA) hsdStype I restriction‑modification system specificity subunit
*NC_0077793,475,5330CT24.6% 392.3 / 57.4 179E10K (GAA→AAA) fabRHTH‑type transcriptional repressor FabR
*NC_0077792,983,3870TG24.6% 238.7 / 17.9 117M288L (ATG→CTG) Y75_RS14830acetyl‑CoA C‑acetyltransferase
*NC_0077793,176,4040CA24.4% 387.0 / 49.1 164E55* (GAG→TAG) nudFADP‑ribose diphosphatase
*NC_0077792,983,3710AT24.2% 268.1 / 39.3 121V293E (GTG→GAG) Y75_RS14830acetyl‑CoA C‑acetyltransferase
*NC_0077792,983,3940CG24.2% 328.7 / 48.2 120P285P (CCG→CCCY75_RS14830acetyl‑CoA C‑acetyltransferase
*NC_0077792,902,3710GA24.1% 368.1 / 82.3 162intergenic (+341/+1032)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 381401 =NA (NA)123 (0.580) 105/492 3.6 NA coding (512/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381812 NA (NA)pseudogene (174/174 nt) Y75_RS23175 acetyltransferase
* ? NC_007779 4310363 =NA (NA)126 (0.590) 99/496 4.3 96.2% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 = 4540919 5 (0.020)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 = 2069490218 (1.030)115 (0.540) 24/496 22.2 50.8% intergenic (‑32/+1) Y75_RS10470/Y75_RS24935 IS5‑like element IS5 family transposase/hypothetical protein
?NC_007779 = 4540919 5 (0.020)intergenic (+208/+375) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 = 4610846129 (0.610)9 (0.070) 7/318 24.2 8.8% intergenic (‑1/+34) Y75_RS22835/Y75_RS22840 tRNA‑Leu/tRNA‑Leu
?NC_007779 4611083 = 104 (0.760)intergenic (‑2/+266) Y75_RS22845/rsmC tRNA‑Leu/16S rRNA (guanine(1207)‑N(2))‑methyltransferase RsmC
* ? NC_007779 = 697163154 (0.720)8 (0.060) 8/340 24.6 6.7% intergenic (‑1/+15) Y75_RS03450/Y75_RS03455 tRNA‑Met/tRNA‑Gln
?NC_007779 697557 = 118 (0.810)intergenic (‑2/+378) Y75_RS03470/asnB tRNA‑Met/asparagine synthase B
* ? NC_007779 1724330 =188 (0.880)3 (0.010) 3/474 35.5 1.6% coding (496/2013 nt) Y75_RS08665 FUSC family protein
?NC_007779 = 1724326 184 (0.910)coding (492/2013 nt) Y75_RS08665 FUSC family protein
* ? NC_007779 2891334 =170 (0.800)3 (0.010) 3/478 35.7 1.8% coding (22/1272 nt) Y75_RS14425 FAD‑dependent oxidoreductase
?NC_007779 = 2891330 167 (0.820)coding (18/1272 nt) Y75_RS14425 FAD‑dependent oxidoreductase