breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 52 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077791,097,5800CT41.1% 44.9 / 86.3 74intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_00777929,4960TA30.0% 188.9 / 40.1 90intergenic (+301/‑155)dapB/Y75_RS001654‑hydroxy‑tetrahydrodipicolinate reductase/glutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
*NC_0077793,205,0910CT29.4% 122.6 / 20.8 69intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077792,004,0550AC29.3% 163.5 / 11.0 75intergenic (‑129/+192)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_0077792,902,3420GT28.9% 301.5 / 27.2 114intergenic (+312/+1061)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077792,488,7000GA28.5% 354.1 / 46.0 165S29L (TCA→TTA) emrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
*NC_0077792,902,3480CA28.2% 262.3 / 49.9 110intergenic (+318/+1055)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077791,826,7750GA27.7% 171.1 / 34.6 83intergenic (‑124/+79)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
*NC_0077794,291,3090TA27.6% 233.6 / 34.8 105intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_0077792,902,3710GA27.1% 237.6 / 69.3 119intergenic (+341/+1032)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077792,391,5320GA27.0% 139.5 / 41.2 74intergenic (+31/‑72)Y75_RS11940/Y75_RS11945M28 family peptidase/YfbM family protein
*NC_007779753,5470TA26.9% 259.2 / 32.2 108intergenic (‑330/+60)Y75_RS03720/gltAtype 1 fimbrial protein/citrate (Si)‑synthase
*NC_0077793,764,8940AG26.7% 150.1 / 32.7 86A28A (GCT→GCCY75_RS18670hypothetical protein
*NC_0077793,764,8930GC26.2% 183.5 / 35.4 86L29V (CTG→GTG) Y75_RS18670hypothetical protein
*NC_0077793,894,9060TA26.2% 148.7 / 12.9 65T236S (ACC→TCC) yiaN2,3‑diketo‑L‑gulonate transporter large permease YiaN
*NC_0077791,097,5400CA25.7% 157.6 / 36.8 70intergenic (+289/+85)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077793,764,8860GC25.6% 172.6 / 42.4 89S31C (TCC→TGC) Y75_RS18670hypothetical protein
*NC_0077793,475,5330CT25.5% 227.2 / 31.3 106E10K (GAA→AAA) fabRHTH‑type transcriptional repressor FabR
*NC_0077793,764,8840GA24.7% 181.8 / 25.4 90H32Y (CAT→TAT) Y75_RS18670hypothetical protein
*NC_0077793,404,0730TG24.5% 231.7 / 12.3 94G245G (GGT→GGGY75_RS16905oxidoreductase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 = 294612334 (0.250)4 (0.040) 4/330 16.8 12.2% intergenic (+4/‑30) Y75_RS14670/Y75_RS14675 tRNA‑Met/tRNA‑Met
?NC_007779 2946344 = 35 (0.390)intergenic (+5/+69) Y75_RS14680/Y75_RS14685 tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase AmiC
* ? NC_007779 = 69716396 (0.710)4 (0.040) 4/340 17.2 5.4% intergenic (‑1/+15) Y75_RS03450/Y75_RS03455 tRNA‑Met/tRNA‑Gln
?NC_007779 697557 = 75 (0.810)intergenic (‑2/+378) Y75_RS03470/asnB tRNA‑Met/asparagine synthase B
* ? NC_007779 1413613 =154 (1.140)3 (0.020) 3/444 21.4 2.3% coding (50/936 nt) ttcA tRNA 2‑thiocytidine(32) synthetase TtcA
?NC_007779 = 1436672 118 (0.970)intergenic (+39/+227) Y75_RS24865/uspF hypothetical protein/universal stress protein UspF
* ? NC_007779 4330520 =114 (0.840)8 (0.060)
+AAC
3/490 22.6 6.5% intergenic (‑101/+557) yjdN/Y75_RS21430 VOC family metalloprotein YjdN/phnA family protein
?NC_007779 = 4331016 119 (0.880)intergenic (‑597/+61) yjdN/Y75_RS21430 VOC family metalloprotein YjdN/phnA family protein
* ? NC_007779 = 1476754142 (1.050)3 (0.020) 3/488 22.6 2.2% coding (820/861 nt) pdxI pyridoxine 4‑dehydrogenase
?NC_007779 4112670 = 126 (0.950)coding (1278/2136 nt) igaA intracellular growth attenuator protein IgaA