Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779 3,948,766 G→A R320H (CGT→CAT)  bcsB → cellulose biosynthesis cyclic di‑GMP‑binding regulatory protein BcsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077793,948,7660GA99.6% 848.9 / ‑4.7 220R320H (CGT→CAT) bcsBcellulose biosynthesis cyclic di‑GMP‑binding regulatory protein BcsB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (104/115);  total (104/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 223 total aligned reads displayed.

GCGCCGGATGTTGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCGTGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAACCTGCCGCCGGATCT  >  NC_007779/3948519‑3949012
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
  gcCGGATGTTGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCCTCCGCAGAATCCTTACGTCAAACTGCTGGGGGTGGTTGGTCATgac                                                                                                                                                                                                                                                    >  2:1765584/1‑250 (MQ=255)
    cGGATGTTGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATgacga                                                                                                                                                                                                                                                  >  1:1896186/1‑250 (MQ=255)
    cGGATGTTGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATgacga                                                                                                                                                                                                                                                  >  1:2002323/1‑250 (MQ=255)
     ggATGTTGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATgacgac                                                                                                                                                                                                                                                 <  1:1881322/250‑1 (MQ=255)
       aTGTTGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACaa                                                                                                                                                                                                                                               <  1:960372/250‑1 (MQ=255)
       aTGTTGGGCTGCAACAAGCCTCTACCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATgacgaaca                                                                                                                                                                                                                                               >  2:1300029/1‑247 (MQ=255)
           tGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGAc                                                                                                                                                                                                                                           >  2:57407/1‑250 (MQ=255)
               cTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGt                                                                                                                                                                                                                                       <  1:420429/250‑1 (MQ=255)
                    acaaGCCGCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTgcag                                                                                                                                                                                                                                  <  1:432930/250‑1 (MQ=255)
                        gCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTgcaggcag                                                                                                                                                                                                                              >  1:1006009/1‑250 (MQ=255)
                             tGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGaaa                                                                                                                                                                                                                         <  1:1402215/250‑1 (MQ=255)
                             tGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGaaa                                                                                                                                                                                                                         <  2:1723552/250‑1 (MQ=255)
                             tGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGaaa                                                                                                                                                                                                                         <  1:173408/250‑1 (MQ=255)
                              gCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAg                                                                                                                                                                                                                        <  2:323846/250‑1 (MQ=255)
                                cATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAGGGt                                                                                                                                                                                                                      >  1:1586051/1‑250 (MQ=255)
                                cATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGt                                                                                                                                                                                                                      >  1:1665987/1‑250 (MQ=255)
                                  ttGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTAt                                                                                                                                                                                                                    >  1:542832/1‑250 (MQ=255)
                                   tGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATc                                                                                                                                                                                                                   <  1:518608/250‑1 (MQ=255)
                                    gTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCg                                                                                                                                                                                                                  <  1:1475451/250‑1 (MQ=255)
                                       gCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTTCAGGCAGCGAAAGGTATCGCTc                                                                                                                                                                                                               <  2:722160/250‑1 (MQ=255)
                                       gCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTc                                                                                                                                                                                                               >  1:33221/1‑250 (MQ=255)
                                       gCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTc                                                                                                                                                                                                               <  1:1028966/250‑1 (MQ=255)
                                         cTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAg                                                                                                                                                                                                             >  1:581333/1‑250 (MQ=255)
                                         cTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAg                                                                                                                                                                                                             <  2:1227052/250‑1 (MQ=255)
                                            gTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAggat                                                                                                                                                                                                          <  1:242059/250‑3 (MQ=255)
                                            gTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGt                                                                                                                                                                                                          <  2:1910506/250‑1 (MQ=255)
                                             tggtttggttCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAgggga                                                                                                                                                                                                         >  2:306470/1‑248 (MQ=255)
                                                        gcgTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGGGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTc                                                                                                                                                                                              >  2:1953878/1‑250 (MQ=255)
                                                              tggctggcGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCgtggt                                                                                                                                                                                        <  1:842993/250‑1 (MQ=255)
                                                               ggctggcGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCGTGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCgtggtg                                                                                                                                                                                       <  1:687846/250‑1 (MQ=255)
                                                                gctggcGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGa                                                                                                                                                                                      <  2:880936/250‑1 (MQ=255)
                                                                  tggcGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGaaa                                                                                                                                                                                    <  1:2056965/250‑1 (MQ=255)
                                                                   ggcGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAg                                                                                                                                                                                   >  2:1990506/1‑250 (MQ=255)
                                                                    gcGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGc                                                                                                                                                                                  <  1:1302183/250‑1 (MQ=255)
                                                                     cGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCg                                                                                                                                                                                 <  1:739310/250‑1 (MQ=255)
                                                                          gCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGGAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTa                                                                                                                                                                            <  1:1432187/250‑1 (MQ=255)
                                                                               aCTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGtgaa                                                                                                                                                                       <  2:1030910/250‑1 (MQ=255)
                                                                                     cGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATAAAGt                                                                                                                                                                 <  2:398983/250‑1 (MQ=255)
                                                                                          ctctATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAAc                                                                                                                                                            >  2:1259887/1‑250 (MQ=255)
                                                                                            ctATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCg                                                                                                                                                          <  2:1209409/250‑1 (MQ=255)
                                                                                             tataACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCgc                                                                                                                                                         >  1:1768520/1‑250 (MQ=255)
                                                                                              ataACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCgct                                                                                                                                                        >  1:1510405/1‑250 (MQ=255)
                                                                                                aaCCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCgctgc                                                                                                                                                      >  2:2029255/1‑250 (MQ=255)
                                                                                                  ccAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTa                                                                                                                                                    <  2:702563/250‑1 (MQ=255)
                                                                                                  ccAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTa                                                                                                                                                    <  2:1807009/250‑1 (MQ=255)
                                                                                                     aCTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACcg                                                                                                                                                 <  1:1821838/250‑1 (MQ=255)
                                                                                                          cGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTaa                                                                                                                                            <  2:287504/250‑1 (MQ=255)
                                                                                                           ggATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAg                                                                                                                                           >  1:2079597/1‑250 (MQ=255)
                                                                                                             aTCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGcc                                                                                                                                         >  2:759941/1‑250 (MQ=255)
                                                                                                                  aaTGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACg                                                                                                                                    <  1:312473/250‑1 (MQ=255)
                                                                                                                    tGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGAt                                                                                                                                  <  2:542832/250‑1 (MQ=255)
                                                                                                                     gCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATg                                                                                                                                 >  2:513806/1‑250 (MQ=255)
                                                                                                                              tttGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACt                                                                                                                        <  2:822955/250‑1 (MQ=255)
                                                                                                                                   aaccaacGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGgta                                                                                                                   <  2:1845788/250‑1 (MQ=255)
                                                                                                                                    accaacGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGgtac                                                                                                                  >  2:78124/1‑250 (MQ=255)
                                                                                                                                       aacGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGgtacgta                                                                                                               >  2:637518/1‑250 (MQ=255)
                                                                                                                                       aacGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGgtacgta                                                                                                               >  1:1360210/1‑250 (MQ=255)
                                                                                                                                        acGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGgtacgtac                                                                                                              >  2:204397/1‑250 (MQ=255)
                                                                                                                                             aaaCGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATc                                                                                                         <  1:920831/250‑1 (MQ=255)
                                                                                                                                              aaCGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCg                                                                                                        >  2:427502/1‑250 (MQ=255)
                                                                                                                                                 ggCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTcc                                                                                                     >  2:1099337/1‑250 (MQ=255)
                                                                                                                                                   ccGGGCTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCgg                                                                                                   <  2:302015/250‑1 (MQ=255)
                                                                                                                                                      gACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTca                                                                                                >  1:352449/1‑250 (MQ=255)
                                                                                                                                                      gACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTca                                                                                                <  1:1281272/250‑1 (MQ=255)
                                                                                                                                                         ttCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACAt                                                                                             >  1:1182184/1‑250 (MQ=255)
                                                                                                                                                         ttCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACAt                                                                                             <  1:1930520/250‑1 (MQ=255)
                                                                                                                                                            cTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTg                                                                                          >  1:1691311/1‑250 (MQ=255)
                                                                                                                                                              gcgcGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGc                                                                                        >  1:1065656/1‑250 (MQ=255)
                                                                                                                                                               cgcgATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCg                                                                                       <  1:1218634/250‑1 (MQ=255)
                                                                                                                                                                gcgATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGa                                                                                      >  1:1114435/1‑250 (MQ=255)
                                                                                                                                                                     catcCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGa                                                                                 <  1:1259887/250‑1 (MQ=255)
                                                                                                                                                                          ggcggTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAAcc                                                                            <  1:818947/250‑1 (MQ=255)
                                                                                                                                                                                 aaaGCCCCGGTGATTGAGATGATTAACCATCGGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATgaag                                                                     <  2:1665987/250‑1 (MQ=255)
                                                                                                                                                                                 aaaGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATgaag                                                                     >  2:1602498/1‑250 (MQ=255)
                                                                                                                                                                                  aaGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATgaaga                                                                    <  1:983274/250‑1 (MQ=255)
                                                                                                                                                                                        cGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTACGCCGTGCGGTGCGCCGCACTGGGCACGTACAGCTCGCCCGGTCACATGTGGCGCACTGAAAACATATGAAGCACAGtt                                                              >  2:337596/1‑250 (MQ=255)
                                                                                                                                                                                          gTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGt                                                                                                  >  2:1461021/1‑212 (MQ=255)
                                                                                                                                                                                            gATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACaa                                                          >  2:1146213/1‑250 (MQ=255)
                                                                                                                                                                                             aTTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAAt                                                         <  2:947602/250‑1 (MQ=255)
                                                                                                                                                                                                     gatTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGt                                                 <  1:278372/250‑1 (MQ=255)
                                                                                                                                                                                                       ttAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCt                                               <  2:520651/250‑1 (MQ=255)
                                                                                                                                                                                                         aaCCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTg                                             <  2:1006009/250‑1 (MQ=255)
                                                                                                                                                                                                             aTCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGcc                                         <  2:352449/250‑1 (MQ=255)
                                                                                                                                                                                                                cgaaaaaTCCTTACGTCAAACTGCTGGTGGTATTTGGTCATGACGAAAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCcagc                                      <  2:1517317/245‑1 (MQ=255)
                                                                                                                                                                                                                  cAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCcagcag                                    >  1:1946013/1‑250 (MQ=255)
                                                                                                                                                                                                                  cAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCcagcag                                    >  2:1033910/1‑250 (MQ=255)
                                                                                                                                                                                                                   aGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCagcagc                                   <  2:2079597/250‑1 (MQ=255)
                                                                                                                                                                                                                   aGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCagcagc                                   <  1:369031/250‑1 (MQ=255)
                                                                                                                                                                                                                        ccTCACGTCAAACTGCTGGTGGGGCTTGGTCATGACGACAAAGATCTGTTGCAGGCAGCGAGAGGTATCTCACAGGGTGAAATTCTGTTCCGTGGTGATAGCGCGGTAGTGAATGAAGTCTAACTGATGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGCTCACACTTGGCTAACTGAAAACCTATGAAGAACAGTTACAATCCTGCGGTCTTGAGCCAGCAGCGATTa                              >  2:1424054/1‑250 (MQ=255)
                                                                                                                                                                                                                         cTTACGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTaa                             >  2:929556/1‑250 (MQ=255)
                                                                                                                                                                                                                            aCGTCAAACTGCTGGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGt                          <  1:763947/250‑1 (MQ=255)
                                                                                                                                                                                                                                     tgctgGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTaaa                 <  1:78124/250‑1 (MQ=255)
                                                                                                                                                                                                                                     tgctgGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTaaa                 >  2:1382829/1‑250 (MQ=255)
                                                                                                                                                                                                                                       ctgGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAAcc               >  1:1558964/1‑250 (MQ=255)
                                                                                                                                                                                                                                       ctgGTGGTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATCCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAAcc               >  1:377701/1‑250 (MQ=255)
                                                                                                                                                                                                                                        tggtggTGTTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAACCt              <  1:1596271/250‑1 (MQ=255)
                                                                                                                                                                                                                                              tgtTTGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAACCTgccgcc        >  2:1594906/1‑250 (MQ=255)
                                                                                                                                                                                                                                                tttGGTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAACCTGCCGCCgg      <  1:425754/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAACCTGCCGCCGGAtc   <  1:1389552/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    gTCATGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAACCTGCCGCCGGAtct  <  2:1320655/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
GCGCCGGATGTTGGGCTGCAACAAGCCTCTGCCATTGTCGCCTCGTGGTTTGGTTCGCGTTCTGGCTGGCGTGGGCAGAACTTCCCGGTACTCTATAACCAACTGCCGGATCGCAATGCCATTGTCTTTGCAACCAACGACAAACGGCCGGACTTCCTGCGCGATCATCCGGCGGTAAAAGCCCCGGTGATTGAGATGATTAACCATCCGCAGAATCCTTACGTCAAACTGCTGGTGGTGTTTGGTCGTGACGACAAAGACCTGTTGCAGGCAGCGAAAGGTATCGCTCAGGGTAACATTCTGTTCCGTGGTGAAAGCGTGGTAGTGAATGAAGTGAAACCGCTGCTACCGCGTAAGCCGTACGATGCGCCGAACTGGGTACGTACCGATCGTCCGGTCACATTTGGCGAACTGAAAACCTATGAAGAACAGTTACAATCCAGCGGTCTTGAGCCAGCAGCGATTAACGTTTCGCTAAACCTGCCGCCGGATCT  >  NC_007779/3948519‑3949012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑