breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | SRR3480978_pass_R1.good.fq | 1,472,643 | 366,603,300 | 100.0% | 248.9 bases | 250 bases | 99.5% |
errors | SRR3480978_pass_R2.good.fq | 1,472,643 | 366,603,300 | 100.0% | 248.9 bases | 250 bases | 96.0% |
total | 2,945,286 | 733,206,600 | 100.0% | 248.9 bases | 250 bases | 97.8% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | NC_007779 | 4,646,332 | 158.2 | 2.3 | 100.0% | Escherichia coli str. K-12 substr. W3110, complete sequence. |
total | 4,646,332 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 32255 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 110 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.012 |
reference sequence | pr(no read start) |
---|---|
NC_007779 | 0.74302 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
option | value |
---|---|
Mode | Consensus/Mixed Base |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 10 |
Polymorphism frequency cutoff | 0.2 |
Polymorphism minimum variant coverage each strand | OFF |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | OFF |
Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 03:47:09 15 Feb 2020 | 03:47:51 15 Feb 2020 | 42 seconds |
Read alignment to reference genome | 03:47:52 15 Feb 2020 | 03:57:51 15 Feb 2020 | 9 minutes 59 seconds |
Preprocessing alignments for candidate junction identification | 03:57:51 15 Feb 2020 | 03:58:41 15 Feb 2020 | 50 seconds |
Preliminary analysis of coverage distribution | 03:58:41 15 Feb 2020 | 04:01:08 15 Feb 2020 | 2 minutes 27 seconds |
Identifying junction candidates | 04:01:08 15 Feb 2020 | 04:01:12 15 Feb 2020 | 4 seconds |
Re-alignment to junction candidates | 04:01:12 15 Feb 2020 | 04:03:32 15 Feb 2020 | 2 minutes 20 seconds |
Resolving best read alignments | 04:03:32 15 Feb 2020 | 04:04:52 15 Feb 2020 | 1 minute 20 seconds |
Creating BAM files | 04:04:52 15 Feb 2020 | 04:07:02 15 Feb 2020 | 2 minutes 10 seconds |
Tabulating error counts | 04:07:02 15 Feb 2020 | 04:07:57 15 Feb 2020 | 55 seconds |
Re-calibrating base error rates | 04:07:57 15 Feb 2020 | 04:07:58 15 Feb 2020 | 1 second |
Examining read alignment evidence | 04:07:58 15 Feb 2020 | 04:18:16 15 Feb 2020 | 10 minutes 18 seconds |
Polymorphism statistics | 04:18:16 15 Feb 2020 | 04:18:16 15 Feb 2020 | 0 seconds |
Output | 04:18:16 15 Feb 2020 | 04:18:47 15 Feb 2020 | 31 seconds |
Total | 31 minutes 37 seconds |