Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077794,371,2710A.97.0% 300.4 / 0.2 66pseudogene (183/1304 nt)Y75_RS21600anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (60/4);  total (60/6)
Fisher's exact test for biased strand distribution p-value = 6.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
*NC_0077794,371,2720A.98.5% 302.6 / ‑2.2 66pseudogene (182/1304 nt)Y75_RS21600anaerobic C4‑dicarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (60/4);  total (60/6)
Fisher's exact test for biased strand distribution p-value = 7.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGC  >  NC_007779/4371023‑4371511
                                                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                               
tGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAGaa                                                                                                                                                                                                                                                 <  1:729690/250‑1 (MQ=255)
  cGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGCCCGCCAG‑CAACCTGCATGGCAGaaat                                                                                                                                                                                                                                               >  2:353143/1‑249 (MQ=255)
   ggTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAGaaata                                                                                                                                                                                                                                              <  2:199711/250‑3 (MQ=255)
   ggTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAGaaata                                                                                                                                                                                                                                              <  2:88477/250‑3 (MQ=255)
      gATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAGaaatagcg                                                                                                                                                                                                                                           <  1:1168464/250‑6 (MQ=255)
        tCGCAATCTGCGCGGATACCACTGCAGTAGACAGCAGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTTTCTCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GAGCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGGCCAGACCGCCAG‑CAACCTGCATGGCAGaaatagcggc                                                                                                                                                                                                                                         <  2:986394/250‑8 (MQ=255)
         cGCAATCTGCGCGGATACCACTGCAGGAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGG‑CAC‑GATCGGTGCGAGGA‑TCGGGATGTATTTCGGGTTAC‑GGCGCAGCAGATTTTCTGTCTGATGACCCAGATAGTCCAGGCCGCCAG‑CAACCTGCATGGCAG‑‑AAATAgcggcg                                                                                                                                                                                                                                      <  2:1168407/250‑1 (MQ=255)
            aaTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                                                                                                                                                                                                                   >  2:655984/1‑250 (MQ=255)
             aTCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATaa                                                                                                                                                                                                                                  >  1:187916/1‑250 (MQ=255)
               cTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                                                                                                                                                                                                                                >  2:173687/1‑250 (MQ=255)
               cTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAAcc                                                                                                                                                                                                                                >  1:1312594/1‑250 (MQ=255)
                 gcgcGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc                                                                                                                                                                                                                              <  2:971726/250‑1 (MQ=255)
                  cgcgGATACCACTGCAGTAGACAGCGGGCGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGcc                                                                                                                                                                                                                             <  1:394713/250‑1 (MQ=255)
                     ggATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATg                                                                                                                                                                                                                          >  1:806022/1‑250 (MQ=255)
                                     gACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACAt                                                                                                                                                                                                          >  1:337425/1‑250 (MQ=255)
                                      aCAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATc                                                                                                                                                                                                         >  1:763884/1‑250 (MQ=255)
                                      aCAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATc                                                                                                                                                                                                         >  1:404918/1‑250 (MQ=255)
                                       cAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCg                                                                                                                                                                                                        >  1:1152792/1‑250 (MQ=255)
                                           ggACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCGAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg                                                                                                                                                                                                    >  2:516662/1‑250 (MQ=255)
                                           ggACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg                                                                                                                                                                                                    >  1:1186415/1‑250 (MQ=255)
                                           ggACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACg                                                                                                                                                                                                    >  2:517015/1‑250 (MQ=255)
                                           ggACGGCAAGGTCTAACGCCTTGTCCCTTC‑GCAACTTCAGCGACAACTGGCAGTGCCGCTAGAGAGATGCTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGT‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGAAAACCGCTATGATAATGGAGATGACATCGAACg                                                                                                                                                                                                    >  2:756647/1‑250 (MQ=255)
                                              cGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGa                                                                                                                                                                                                 >  1:302570/1‑250 (MQ=255)
                                                    ggTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTAc                                                                                                                                                                                           >  2:1404629/1‑250 (MQ=255)
                                                        tAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCggg                                                                                                                                                                                       >  1:1123859/1‑250 (MQ=255)
                                                          aCGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt                                                                                                                                                                                     >  1:1135475/1‑250 (MQ=255)
                                                             ccTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTaa                                                                                                                                                                                  >  1:1210103/1‑250 (MQ=255)
                                                              cTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGGGATGACATAGAACAGGATGTTACCGGGTTTAAc                                                                                                                                                                                 >  2:659273/1‑250 (MQ=255)
                                                                tGTTCCCTCGGCACCTCCAGCTAAAACTGGCGGCGTCGCCAGAGACATTTTCCCGGTCCCCGCAAGGATCGGC‑GGAAATGAGGC‑CAC‑GATGGGT‑CGAGGATTGGTGATTTTTTCCGTTTTCC‑GGCGACGAAGTTTTTCTAGCTGATAACCAAGATGGCCCACCCCGCCAC‑CACCGTGAATGGC‑‑AAAAATAGGGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACg                                                                                                                                                                                <  2:440872/250‑1 (MQ=255)
                                                                   tCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCaa                                                                                                                                                                            >  1:445689/1‑250 (MQ=255)
                                                                   tCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCaa                                                                                                                                                                            >  1:1229069/1‑250 (MQ=255)
                                                                          gCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATACTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATTACATCGAACGCGATGTTACCGGGTTTAACGCCAATAGCgg                                                                                                                                                                      >  2:1362653/1‑250 (MQ=255)
                                                                                tCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGaa                                                                                                                                                                >  1:801746/1‑250 (MQ=255)
                                                                                     gATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGc                                                                                                                                                           >  1:703678/1‑250 (MQ=255)
                                                                                      gaaaCTGGCAGTGGCGGCAGAGAGGGTGTGGCAGTAACAAGAAAGATAGGTAAGAAAT‑AGGT‑CAA‑GGAGGGTGGGGGGG‑TCGTGGTGTTTTTTGGGTTGG‑GGGCGAGGCGTTTTTTCGGCGGAATGACCAAATAGGCCAGCCCCGCAGCCACCCTGGATGG‑‑GAGAAATGGGGGCGAAAGCCGCCATGATAATGGAGATGACGTCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGc                                                                                                                                                           <  2:92121/248‑1 (MQ=255)
                                                                                       tAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAc                                                                                                                                                         >  1:1334226/1‑250 (MQ=255)
                                                                                        aaCTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCAcc                                                                                                                                                        >  1:271307/1‑250 (MQ=255)
                                                                                                     gCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTg                                                                                                                                           >  1:1190584/1‑250 (MQ=255)
                                                                                                         gagagaTGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCaaa                                                                                                                                       >  2:984931/1‑250 (MQ=255)
                                                                                                           gagaTGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAAc                                                                                                                                     >  1:143338/1‑250 (MQ=255)
                                                                                                                   gCCAGTCACAGCAAAGATGGT‑AGGAAAA‑TGGTCCAC‑GTGGGGGGGGGGGT‑TGGTGTTGTATTT‑GGGTTCCGGGCGCAGCTGTTTTTTTTGTTGAAGACCAAGATACCCCAGCCCGCCAC‑CACCCTG‑AAGGGA‑AAAAATAGGGGAAATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCt                                                                                                                             <  2:1152792/250‑1 (MQ=255)
                                                                                                                    ccagacccAGCAAAGATGGTCAGGAAAT‑AGGT‑CAC‑GAGCGGGGGGAGGA‑TCGTGATGTATTCCGGGTTCC‑GGCGCAGAACCTTTTCTGTCTGATGAACCAGTACGTCCAGACCGCCAG‑CAACCGGCATGGCAG‑‑AAATAGCGGCGATAGCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTa                                                                                                                            <  2:1413843/244‑1 (MQ=255)
                                                                                                                    acaaaacaagaaaaGATAGTAAGGAAAT‑AGG‑‑CACGGCGGGGTGGGGGGA‑TGGTGTTTTTTTTCGGGTACG‑GGCGCCGCTTTTTTTGTTTCTAAAAACCAAGAAAGCCCAGCCCCCCAG‑CACCCTGCATGG‑A‑AAAAATGGCGGCGAAAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTa                                                                                                                            <  2:760665/239‑1 (MQ=255)
                                                                                                                     cAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTAt                                                                                                                           >  2:1154758/1‑250 (MQ=255)
                                                                                                                      aGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTAtt                                                                                                                          >  2:432930/1‑250 (MQ=255)
                                                                                                                        tACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTcc                                                                                                                        >  2:875982/1‑250 (MQ=255)
                                                                                                                          ccAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGTA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTcccc                                                                                                                      >  1:704773/1‑250 (MQ=255)
                                                                                                                              cAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GCTCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCCGCAAAACCAATACCTATTACCCCCac                                                                                                                  >  2:562675/1‑249 (MQ=255)
                                                                                                                                 aGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCt                                                                                                               >  2:1059048/1‑250 (MQ=255)
                                                                                                                                           gAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGCTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTCCCGGGTTTAAAGCCAATAGCGGCAAGAACCAGCACTCCCAATCCGCCTGCAAAACCAATACCTATTTCCCCAAACCGGGCGCCCaag                                                                                                     >  2:571261/1‑250 (MQ=255)
                                                                                                                                             aaT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCaagaa                                                                                                   >  2:1066074/1‑250 (MQ=255)
                                                                                                                                                 aGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGAt                                                                                                >  1:472601/1‑250 (MQ=255)
                                                                                                                                                 aGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGAt                                                                                                >  1:1131101/1‑250 (MQ=255)
                                                                                                                                                        c‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAg                                                                                          >  2:220454/1‑250 (MQ=255)
                                                                                                                                                          gATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGc                                                                                         >  1:42839/1‑250 (MQ=255)
                                                                                                                                                           aTCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCa                                                                                        >  1:1362076/1‑250 (MQ=255)
                                                                                                                                                             cGGTGCGAGGA‑TCGTGATGTATTTCGCGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAATCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCTCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCACTAGCGGCAAGAACCGGCACCCCCCATCCGCCTGCAAAACACATACCTATTCCCCCCAATCTGGCGCCCAAGAATATCGCCAGCaaa                                                                                      >  2:621165/1‑250 (MQ=255)
                                                                                                                                                                  cGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTat                                                                                 >  2:341964/1‑250 (MQ=255)
                                                                                                                                                                      gA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTatgatg                                                                             >  1:1378578/1‑250 (MQ=255)
                                                                                                                                                                      gA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTatgatg                                                                             >  1:782338/1‑250 (MQ=255)
                                                                                                                                                                                  aTTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAAc                                                                  >  1:1321303/1‑250 (MQ=255)
                                                                                                                                                                                         gTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGAAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCata                                                           >  1:471880/1‑250 (MQ=255)
                                                                                                                                                                                           tAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATAtt                                                         >  1:133467/1‑250 (MQ=255)
                                                                                                                                                                                             c‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAg                                                       >  1:1038290/1‑250 (MQ=255)
                                                                                                                                                                                                       agcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTg                                              >  1:524142/1‑250 (MQ=255)
                                                                                                                                                                                                                   tgatgaACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAg                                  >  2:1438607/1‑250 (MQ=255)
                                                                                                                                                                                                                   tgagaacccAGATAGCCCAGCCCGCCAG‑CAACCTGCATGGC‑‑AAAAAGGGGGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAg                                  <  1:1440746/243‑1 (MQ=255)
                                                                                                                                                                                                                   atgagaACCAGATAGCCCCGGCCCCCAA‑GAACCCGGA‑GGGAAAAAAAGGGCGAGAATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTGTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACaa                                   <  2:1378578/246‑1 (MQ=255)
                                                                                                                                                                                                                        aaCCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGat                             >  1:48676/1‑250 (MQ=255)
                                                                                                                                                                                                                          ccAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGatat                           >  2:325851/1‑250 (MQ=255)
                                                                                                                                                                                                                          caggATAGTCACGCCCGCCAG‑AACCCTGCA‑GGCA‑AGAAATGGCGGCAATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGatat                           <  2:445689/247‑1 (MQ=255)
                                                                                                                                                                                                                             gATAGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCTCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCACGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACCAGTTGATATTAg                        >  2:427563/1‑250 (MQ=255)
                                                                                                                                                                                                                                aGTCCAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGAtt                     >  2:726402/1‑250 (MQ=255)
                                                                                                                                                                                                                                  cccaaccccccAG‑CACCCGGCATGG‑A‑AAAAATAGGGGCAAAAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGt                   <  1:907751/241‑1 (MQ=255)
                                                                                                                                                                                                                                  cccAGCCCGCCAG‑CACCCTGCATGG‑A‑AAAAATGGCGGCGATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGt                   <  1:629215/249‑1 (MQ=255)
                                                                                                                                                                                                                                   ccAGACCGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGtt                  >  1:203085/1‑250 (MQ=255)
                                                                                                                                                                                                                                    cAGACCGCCAG‑AAACCGGAAGGACAGAAAA‑‑AGCGGGAATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTa                 <  1:1342746/250‑1 (MQ=255)
                                                                                                                                                                                                                                         cGCCAG‑CAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTAttttt            >  2:1353695/1‑250 (MQ=255)
                                                                                                                                                                                                                                          gCCAG‑CAACCTGCA‑GG‑AAAAAAAGGGCGGAAAAAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTa           <  1:62785/250‑1 (MQ=255)
                                                                                                                                                                                                                                                cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTa      >  2:1135087/1‑250 (MQ=255)
                                                                                                                                                                                                                                                cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTa      >  1:1367236/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 aaCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTAc     >  1:957074/1‑250 (MQ=255)
                                                                                                                                                                                                                                                      ctgcagggaaaaaaGAGGGGCAATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGc  <  1:1208143/241‑1 (MQ=255)
                                                                                                                                                                                                                                                               ||                                                                                                                                                                                                                                               
TGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTC‑GCAACTTCAGCGATAACTGGCAGTGTCGCCAGAGAGATGTTGCCAGTACCAGCAAAGATAGTCAGGAAAT‑AGGT‑CAC‑GATCGGTGCGAGGA‑TCGTGATGTATTTCGGGTTAC‑GGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAG‑CAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAACGCCAATAGCGGCAAGAACCAGCACCCCCAATCCGCCTGCAAAACCAATACCTATTCCCCCCAATCTGGCGCCCAAGAAGATCGCCAGCAAAACTATGATGAGTTCTACAACTAGCATATTAGCCTTCCTTGTTTTTTAACAAGTTGATATTAGATTGTTATTTTTAAGTTACTGC  >  NC_007779/4371023‑4371511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑