Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077792,488,7000GA23.2% 276.2 / 24.0 112S29L (TCA→TTA) emrKmultidrug efflux MFS transporter periplasmic adaptor subunit EmrK
Reads supporting (aligned to +/- strand):  ref base G (65/21);  new base A (0/26);  total (65/47)
Fisher's exact test for biased strand distribution p-value = 6.21e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

TATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTGGCTTT  >  NC_007779/2488451‑2488948
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
tataGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTg                                                                                                                                                                                                                                                           <  2:961368/250‑1 (MQ=255)
 ataGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGa                                                                                                                                                                                                                                                          >  1:293963/1‑250 (MQ=255)
    gTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGaaaa                                                                                                                                                                                                                                                       <  2:23338/250‑1 (MQ=255)
     tttATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGCTTAACGACAGGGACACTACCTGAGACTTGTGCAGATATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATATGCATAGGCACCTGAAAAc                                                                                                                                                                                                                                                      >  1:459988/1‑250 (MQ=255)
     tttATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAAc                                                                                                                                                                                                                                                      <  2:47816/250‑1 (MQ=255)
      ttATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACg                                                                                                                                                                                                                                                     <  2:275379/250‑1 (MQ=255)
            gTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑aataa                                                                                                                                                                                                                                              >  2:493983/1‑250 (MQ=255)
            gTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑aataa                                                                                                                                                                                                                                              >  2:448016/1‑250 (MQ=255)
             tttGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑aataaa                                                                                                                                                                                                                                             >  1:619054/1‑250 (MQ=255)
             tttGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑aataaa                                                                                                                                                                                                                                             <  1:18557/250‑1 (MQ=255)
                  cGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCTCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATATGCATAGGTACCTGAAAACGC‑AATAAATaaaa                                                                                                                                                                                                                                        >  2:863706/1‑250 (MQ=255)
                  cGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATaaaa                                                                                                                                                                                                                                        <  1:1002373/250‑1 (MQ=255)
                  cGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATaaaa                                                                                                                                                                                                                                        <  2:11043/250‑1 (MQ=255)
                   gAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAAc                                                                                                                                                                                                                                       <  1:695800/250‑1 (MQ=255)
                        atatTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAGAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCg                                                                                                                                                                                                                                  <  2:150280/250‑1 (MQ=255)
                        atatTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCg                                                                                                                                                                                                                                  >  2:169868/1‑250 (MQ=255)
                          atTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGcc                                                                                                                                                                                                                                <  2:526491/250‑1 (MQ=255)
                           tttGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCa                                                                                                                                                                                                                               <  2:343085/250‑1 (MQ=255)
                                  gATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGa                                                                                                                                                                                                                        >  1:426408/1‑250 (MQ=255)
                                        ttttAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTa                                                                                                                                                                                                                  >  1:669157/1‑250 (MQ=255)
                                            aGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTAtttt                                                                                                                                                                                                              >  2:585538/1‑250 (MQ=255)
                                                    tGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGGTCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt                                                                                                                                                                                                      >  1:485191/1‑250 (MQ=255)
                                                        tGCGATATGGGCATCAGTTTTATCCAGAAAAACTAAAATGTCACCTTGCCGAACGTAGTTCGTATTTTTATGATTAACGACAGTGCCACTCCCTGAGTTTTGTGAAGAAATTGGATCTGCTTCCCCCGTGACATAGGCTTCATCTGTACAAATCATTTCTTCAAATCCCATTGACAAATAGGCAAAGACACCTAAAACCGC‑AAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa                                                                                                                                                                                                  <  2:987106/250‑1 (MQ=255)
                                                         gCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                                                                                                                 >  2:734619/1‑250 (MQ=255)
                                                           gATAGTGGCATCAGTTTTATCCAGTGAAACAAAAATTTCCCCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGATTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTAAAATTCCATTGACCAATAGGCAAAGGCACCTAAAAACGA‑AAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt                                                                                                                                                                                               <  2:293963/250‑1 (MQ=255)
                                                            aTAGTGGCATCATTTTTACCCAGTGAAACTAAATTGTCACTTTGTCGAACGTAGTTCTTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGAAGAAATTGGATCTGCTTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGACAAATAGGCATAGGCACCTGAAAACGA‑AAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtt                                                                                                                                                                                              <  1:986393/250‑1 (MQ=255)
                                                              tgTGGCATCATTTTTATCCAGTAAAACTAAAATGTCCCCTTTTCGAACGTAGTTTGTATCTTTATGATAAACGACAGTGACACTACCTGAGACTTGTGCAAAAATTGGATCTCCTTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCAAATTCAATTGACCAAAAGGCATAGGCACCTAAAAACGA‑AATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt                                                                                                                                                                                            <  2:973521/249‑1 (MQ=255)
                                                                    aTCAGTTTTTTCAATAAAAACAAAAAGGTCACGTTGGCGACTTTAGTTGGTATTTTTATGATAAACAACAGTGACACTCCGTGAGCTTTGTGCAAAAATGGGTTCTCCTTTCCCGGTAAAATAGGCGTCATTTGTACTAATCATGTCTTAAAATTCAATTAACCAATAGGAATAGCCACTTAAAAACGC‑AAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                                                                                                                                      <  2:153849/250‑1 (MQ=255)
                                                                     tCATTTTTATCCAGTAAAAAAAAAATGTCACTTTGTCGAAGTTAGTTCTTATTTTTATTATTACCGACAGAGCCACTCCCTGAGATTTGTGAAAAAATTGGATCTCCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTTTTTTAATCCCATTGCCAAAAAGAAAAAGGCACCTAAAACCGA‑AATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTg                                                                                                                                                                                     <  2:54248/250‑1 (MQ=255)
                                                                      cAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTATAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAAATACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa                                                                                                                                                                                    >  1:234498/1‑250 (MQ=255)
                                                                                    gAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                                                                                                      >  2:1103979/1‑250 (MQ=255)
                                                                                    cgatcTAAAATGCCCCCTTGTCGAATGTAGTTCGTATTTTTATGATTAACGACAGTGACCCTCCCTGATCTTTGTGCAAAAATTGGTTCTGCTTCCCCCGTAACATAGGCGTCATTTTTACTAATAATTTTTTATAATCCCATTGCCAAATAGGCATAGGCACTTAAAAACGA‑AAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                                                                                                      <  2:976153/246‑1 (MQ=255)
                                                                                    aaaaTTAAAATGCCTCCTTGTCGAACGTAGTTCGTTTTTTTATGATTAACGACAGGGACACTCCCTGAGACTTGTACAAAAATTGGATCTTCTTTCCCCGTAAAATAGGCGTCATCTACACTAATCATGTTTTCAAATTCTATTGACCAAAAGGAATAGGCACCTAAAAACGA‑AATAAAAAAAACTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                                                                                                      <  2:1093129/249‑1 (MQ=255)
                                                                                      aaCTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                                                                                                                                    <  1:69907/250‑1 (MQ=255)
                                                                                        cTAAAATGTCACCTTGTCGAACGTAGTTCGTATTTTTATGATTAACGACAGTGACACTCCCTGAGACTTGTGCAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTAAAAACGA‑AATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtat                                                                                                                                                                  <  1:153267/250‑1 (MQ=255)
                                                                                          aaaaTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCaagatcg                                                                                                                                                                >  1:976153/1‑244 (MQ=255)
                                                                                          aaaaTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatta                                                                                                                                                                >  1:960187/1‑250 (MQ=255)
                                                                                           aaaTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat                                                                                                                                                               >  1:959448/1‑250 (MQ=255)
                                                                                           aaaTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGACAAATAGGCATAGGCACCTAAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat                                                                                                                                                               <  1:169868/250‑1 (MQ=255)
                                                                                                      tGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                                                                                                                                    >  2:370559/1‑250 (MQ=255)
                                                                                                         cGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                                                                                                                 >  1:962571/1‑250 (MQ=255)
                                                                                                         cGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                                                                                                                 >  2:300313/1‑250 (MQ=255)
                                                                                                          gAACGTAGTTCGTATTTTTATGATTAACGACAGTGACACTACCTGAGATTTGAGAAGAAATGGGATCTGCATTCCCCGTGACAGAGGCGTCATCTGTACAAATAATGTCTTAAAATTCCATTGACAAATAGGAATAGGCACCTGAAAACGA‑AAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATa                                                                                                                                                <  1:728094/250‑1 (MQ=255)
                                                                                                           aaCGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAg                                                                                                                                               >  1:171955/1‑250 (MQ=255)
                                                                                                           aaCGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATATTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTTTCATAg                                                                                                                                               >  2:115981/1‑250 (MQ=255)
                                                                                                             cGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGAt                                                                                                                                             >  2:292815/1‑250 (MQ=255)
                                                                                                              gTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                                                                                                            >  2:692685/1‑250 (MQ=255)
                                                                                                              gTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                                                                                                            >  1:254680/1‑250 (MQ=255)
                                                                                                                    cGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAttt                                                                                                                                      >  1:608043/1‑250 (MQ=255)
                                                                                                                     gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTa                                                                                                                                     >  2:874646/1‑250 (MQ=255)
                                                                                                                        tttttATGATTAACGACATTCCCACTCCCGGATCCTTTTGCAGAATTTGAATCTGCTTCCCCCGTTACAAGGGCTTCATCTGTACTAATCATTTCTTCTAATTCCATTACCCAATAGAAATAGCCACCTAAAACCTC‑AATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga                                                                                                                                  <  2:426408/248‑1 (MQ=255)
                                                                                                                         cTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaa                                                                                                                                 >  2:1103723/1‑250 (MQ=255)
                                                                                                                           ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATATGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                                                                                                                               >  2:870007/1‑250 (MQ=255)
                                                                                                                             aTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattga                                                                                                                             >  1:991057/1‑250 (MQ=255)
                                                                                                                                aTTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatg                                                                                                                          >  1:126446/1‑250 (MQ=255)
                                                                                                                                    aCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaaa                                                                                                                      >  1:379163/1‑250 (MQ=255)
                                                                                                                                       aCAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAagag                                                                                                                   <  1:493983/250‑1 (MQ=255)
                                                                                                                                        cAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGc                                                                                                                  >  1:237321/1‑250 (MQ=255)
                                                                                                                                              cacTACCTGAGACTTGTGCAAAAATTGGATCTGCATCCCCCGTGACATAGGCTTCATCTGAACAAATCATTTCTTCTAATTCCATTGCCAAAAAGGAAAAGCCACCAGAAACCGA‑AAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcat                                                                                                            <  1:326874/250‑1 (MQ=255)
                                                                                                                                               acTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcatt                                                                                                           >  2:405137/1‑250 (MQ=255)
                                                                                                                                               acTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcatt                                                                                                           >  2:342998/1‑250 (MQ=255)
                                                                                                                                               acTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcatt                                                                                                           >  2:333372/1‑250 (MQ=255)
                                                                                                                                                cTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCattcattc                                                                                                          >  1:452260/1‑250 (MQ=255)
                                                                                                                                                   ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATG‑AAAGAGCATTCATTCTTCa                                                                                                      >  2:908755/1‑250 (MQ=255)
                                                                                                                                                             gtgCAAAAATTGTATCTGCATCCCCCTTGACATAGGCGTCATCTGAACTAATCATGTCTTCTAATCCCATGGACCAATAGAAATAGGCCCCTAAAACCGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAaca                                                                                             <  1:683972/250‑1 (MQ=255)
                                                                                                                                                             gggaaGAAATGGGATCTGCATGCCCCGGGACATGGGCTTCATTTATACTAATCATGTCTTTAAATTCCATTGCCCAATAGGCATAGGCCCCTAAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAaca                                                                                             <  1:826243/246‑1 (MQ=255)
                                                                                                                                                               gCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAAcaca                                                                                           >  1:673403/1‑250 (MQ=255)
                                                                                                                                                               acAGAATTTGGATCTGCATTCCCCGTGACATAGGATTCATCTGTACTAATCATGTCTTATAATTCCATTGACCAATAGACAAAGGCACTTAAAAACGA‑AATAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAAcaca                                                                                           <  2:588525/249‑1 (MQ=255)
                                                                                                                                                                      ttGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCAtg                                                                                    >  1:846388/1‑250 (MQ=255)
                                                                                                                                                                      ttGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCAtg                                                                                    >  2:486135/1‑250 (MQ=255)
                                                                                                                                                                         gATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATgtgt                                                                                 >  1:57490/1‑250 (MQ=255)
                                                                                                                                                                          aTCTGCTTTCCCCGTGACATAGGCGTCATCTGTACTAAACATGTCTTCTAATCCCATTGACAAATAGGCATAGGCACCTAAAAACGC‑AAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGtt                                                                                <  1:321532/250‑1 (MQ=255)
                                                                                                                                                                            cTGCATTCCCCGTGACATAGGCTTCATCTGTACTAATCATGTCTTCTAATTCCATTAACCAATAGAAAAAGGCACCTAAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGc                                                                              <  2:154128/250‑1 (MQ=255)
                                                                                                                                                                                aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACAt                                                                          >  2:331860/1‑250 (MQ=255)
                                                                                                                                                                                aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACAt                                                                          >  1:156801/1‑250 (MQ=255)
                                                                                                                                                                                   ccccGTGACATAGGCGTCATCTGTACTAATAATGTCTTCTAATCCCATTCACCAATAGGCAAAGGCACCTAAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTAt                                                                       <  2:485191/250‑1 (MQ=255)
                                                                                                                                                                                   ccccATAACATAGGCTTCAGCTGTACAAATAATTTCTCTAAATTCAATTAACAAATAGACAAAGCAACCTAAAACCAA‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTAt                                                                       <  2:39843/250‑1 (MQ=255)
                                                                                                                                                                                      cGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATccc                                                                    >  2:126029/1‑250 (MQ=255)
                                                                                                                                                                                         gACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAgg                                                                 >  1:596197/1‑250 (MQ=255)
                                                                                                                                                                                               ggCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGACATAGGCACCTGAAAACAC‑AATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGtt                                                           <  1:241754/250‑1 (MQ=255)
                                                                                                                                                                                                gCGTCTTCTGTACTAATCATGTCTTCTAATCCCATTGACCAATAGGCATAGGCACCTAAAAACGA‑AATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTg                                                          <  1:151584/250‑1 (MQ=255)
                                                                                                                                                                                                   tCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAg                                                       >  2:246438/1‑250 (MQ=255)
                                                                                                                                                                                                    cATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAgg                                                      >  2:948894/1‑250 (MQ=255)
                                                                                                                                                                                                           aCTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAAtt                                               >  1:99978/1‑250 (MQ=255)
                                                                                                                                                                                                                 cATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATg                                         >  1:1085481/1‑250 (MQ=255)
                                                                                                                                                                                                                      cttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTg                                    <  1:185658/250‑1 (MQ=255)
                                                                                                                                                                                                                       ttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGc                                   >  2:458230/1‑250 (MQ=255)
                                                                                                                                                                                                                          tAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCaaa                                >  1:534297/1‑250 (MQ=255)
                                                                                                                                                                                                                           aaTTCCATTGACCAATAGGCA‑AAGCACCCTAAAAAGCAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCaaaa                               <  2:410139/250‑1 (MQ=255)
                                                                                                                                                                                                                                aatcgACCAAAAGGAATAGCCCCGTAAAAATGC‑AAGAAATAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAaac                          <  2:794015/246‑1 (MQ=255)
                                                                                                                                                                                                                                  ttGACCAATAGGCATAGACACCTAAAAACAC‑AAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAaacaa                        <  2:608043/250‑1 (MQ=255)
                                                                                                                                                                                                                                       cAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACagag                   <  1:585538/250‑1 (MQ=255)
                                                                                                                                                                                                                                         ataggcataggcACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGGATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAACAAACAACAGAGGa                 >  2:798465/1‑250 (MQ=255)
                                                                                                                                                                                                                                           aggcataggcaCCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACt               >  2:888170/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    caCCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTgg      >  1:1033806/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    caCCTGAAAACGA‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTgg      <  1:874646/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     aCCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAACTGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTGGc     >  2:546999/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       cTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTGGCtt   >  2:282733/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        tGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCACAAAAAACAACAGAGGACTAAACCGTGGCttt  >  2:828944/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        tGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTGGCttt  >  1:630314/1‑250 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
TATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGCATGTGTTGCACATTATCCCAGGAACGTTGGAGGCCTAATTTAAATGAGGTGCAAAAAAAACAACAGAGGACTAAACCGTGGCTTT  >  NC_007779/2488451‑2488948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑