| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_007779 | 4,609,024 | 0 | C | T | 31.2% | 134.1 / 37.8 | 80 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (8/47); new base T (25/0); total (33/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATC > NC_007779/4608775‑4609269 | tGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATT‑AGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttct < 1:687859/250‑1 (MQ=255) tAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt > 1:242495/1‑250 (MQ=255) tAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt > 1:263442/1‑250 (MQ=255) aaaTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt < 1:676279/250‑1 (MQ=255) aagaGCGCGAAGGGTGCAAGAGAACTTAACTTGCCTTCGGTTTATGGAGAAGCCGCAGAATGGAACACAGCCGAAGTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttgttc < 2:552124/247‑1 (MQ=255) aaTAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc < 2:275061/250‑1 (MQ=255) aGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCtttttttttttttttc > 1:731644/1‑246 (MQ=255) gcgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCAt > 1:812273/1‑250 (MQ=255) cgcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg < 1:896248/250‑1 (MQ=255) gcAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga > 1:277696/1‑250 (MQ=255) gggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTTcccttggaa > 2:494675/1‑243 (MQ=255) gggTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa < 1:303132/250‑1 (MQ=255) tGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTg < 2:12443/250‑1 (MQ=255) aaGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGAAGGGAgc > 1:251061/1‑250 (MQ=255) agagAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCCTGTGAAGTGAggg > 1:784418/1‑248 (MQ=255) acttaacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag < 1:1078982/250‑1 (MQ=255) aacttGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg < 2:139616/250‑1 (MQ=255) ccTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc < 1:673548/250‑1 (MQ=255) tGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTACTTTTTTCTTTTTTTTTCCTATGATGTGAGCGCCGAGCGGGAGTTTC‑TTgtc > 2:920494/1‑248 (MQ=255) gTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCct < 1:881438/250‑1 (MQ=255) tATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCctgac < 1:445939/250‑1 (MQ=255) aTGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGAATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCctgact < 1:476587/250‑1 (MQ=255) ccGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTGTTTTTTTTTTTTTCCTATGAATAGTGGGTGCAGGGATGGATTTTC‑TTTTCCTACTTAACTgggg > 1:991572/1‑248 (MQ=255) cAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGAtt < 1:463850/250‑1 (MQ=255) aGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGAGAAGAGAGGGCAGGGAGGGGTTATT‑TTTCCTGACTGAACTGGCGGttt > 2:309928/1‑249 (MQ=255) aGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTa < 1:908881/250‑1 (MQ=255) aTGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGt < 2:350326/250‑1 (MQ=255) aTGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGt < 1:953243/250‑1 (MQ=255) ggAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACATATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATATCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGttt < 2:975677/250‑1 (MQ=255) aaCACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCAAGAGAAGGGAGCGCAGGGGGGGGGTATCTTTGTCTG‑CTGAACTGGCGGTTaatgttgc > 2:614148/1‑244 (MQ=255) acaGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGATAAGTGAGCGCCGAGGGGGGTTTTC‑TTTCCTGACTTAACTGGCGGTTTAGttttctcg > 2:658621/1‑245 (MQ=255) ccGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATC‑TTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGTAcc > 1:615357/1‑250 (MQ=255) gAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTGTTTTTTTTTTTTTTTCCCTGTGGAGTGAGCGTCGAGGGGGGTTATC‑TTTCCTTACTGAACCGGCCATTTTGTTTcgggtctactc > 2:599723/1‑240 (MQ=255) aagaagAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGTGAGCGCAGCGAGGGGTTATT‑TTGTCTGCTTTAACTGGCGATTAAGTTTTCCCGGACCCCGCGtt > 2:73371/1‑250 (MQ=255) aagaagAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt < 2:131221/250‑1 (MQ=255) agaagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGTGAAGTGAGGGCAGAGAGGGAGTATC‑TTGCCTGACTGAACTTGCGGTTAAGTTTCCGCGTACCCGGCGttt > 2:602627/1‑250 (MQ=255) gaagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGACATTTTTTCTTTTTTTGACATGAGCAGAGAGCGCAGAGAGGGATTATC‑TTGTCTGACTGAACCGGGGATTTAGGTTCTGCGTAACCGGCGtttt > 2:730669/1‑249 (MQ=255) aagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTGGAAGTGAGCGCAGAGGGGGGGTTTT‑TTTCCTTACTTAACTGGCGATTAAGTTTTCGCGGACCCCGCGttttg > 2:364126/1‑248 (MQ=255) aagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg < 2:20227/250‑1 (MQ=255) gTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCGTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTGGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg < 2:1103257/250‑1 (MQ=255) gCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg < 1:171304/250‑1 (MQ=255) aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTGGAAGGGAGCGCAGAGGGGGGGTATC‑TTTTCTTACTTGACTGGGGGTTTAGTTTTCGCCTACCCCGCGTTTTGTTATTGCGGGTGATGTTAtttt > 2:109754/1‑248 (MQ=255) aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGTGAAGAGAGCGCCGAGAGGGGTTATT‑TTTCCTTGCTTGACTGGGGGTTAAATTTTCCCGTTGCCGGCGGTTATTTTTTTCGGGTGATGATAtttg > 2:1054319/1‑248 (MQ=255) tGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCTGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTc < 2:744268/250‑1 (MQ=255) tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt < 2:170821/250‑1 (MQ=255) cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCCGAGGGGGATTATC‑TTGTCTGACTGAACTGGCGATTAAGTTTCCGCGCACCCGGCGTTTTGTTTTTTCGGAGGTTGATATTTAAGCGctgctg > 2:319099/1‑250 (MQ=255) agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTg < 1:87518/250‑1 (MQ=255) agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCAGAGAGGGGTTATC‑TTTCCTGACTGAAATGGCGATTAAGTTTTCGCGTACCCGGCGTTTTGTTATTGCGGGTGATGGTTTTTAAGCTCGGGTGAGTTGGt > 2:908458/1‑250 (MQ=255) taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATT‑TTGCCTGACTGGACTGTCGGTTAAGTTTTCGCGTAACCCGCGGTTTGTTTTTTGGGGTGATGTATTTGAAGCTCGGGTGT‑GTGGTTCa > 2:1091856/1‑250 (MQ=255) aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCAt < 2:1029540/250‑1 (MQ=255) agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATc < 2:69444/250‑1 (MQ=255) agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATc < 2:248561/250‑1 (MQ=255) tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGTGAGCGGAGAGAGGGGGTATT‑TTTCCTGTCTGGACTGGCGGTTAAGTTTCCCGGGACCCCGCGGTTTGTTTTTGGGGGTGGTGTTATTGTAGCTCGGCTTG‑TTTGTTTCTTTTTGCCATCAcccgc > 2:1073816/1‑247 (MQ=255) tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCt < 2:1090393/250‑1 (MQ=255) tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCt < 1:494675/250‑1 (MQ=255) aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATC‑TTTCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGGTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTTGTTTATTGTTGCCATCACCGCTG‑GGCg > 1:292575/1‑250 (MQ=255) aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACg < 2:259408/250‑1 (MQ=255) aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGgt < 1:334320/250‑1 (MQ=255) ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTa < 1:33573/250‑1 (MQ=255) ggTAACCCTTATAAGCTCCATACCTATACCACTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTa < 2:832312/250‑1 (MQ=255) gTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTaa < 2:561837/250‑1 (MQ=255) tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTaaa < 2:263442/250‑1 (MQ=255) aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAg < 1:309928/250‑1 (MQ=255) cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGGGGGGGGTTTTT‑TTTCCTGACTTGACTGGCGGTTTAGGTTTCGCGGACCCGGCGGTTTGTTTTTTCGGGTGGTGGTTTTTAAGTCCGGCTTA‑TTGGGTTATTTTTGCCATTACCGCTT‑GGCGGGGGATTAAGTAAAACGTCCACggctg > 2:820564/1‑246 (MQ=255) cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGTTTAGTTATTGCGGGTGATGATATTTAAGCTCGGCTGG‑TTGGTTCATTTTCGCCATCACCGCTG‑GGCGGTGGATTAAGTAAAGCGTCAACGCTgg > 1:154196/1‑250 (MQ=255) ccATCCCTATACCAGTCAGGATTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTGCCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGgag < 2:467855/250‑1 (MQ=255) ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGgag < 1:50629/250‑1 (MQ=255) ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGgag < 2:277696/250‑1 (MQ=255) cATACCTATACCATTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGgaga < 2:501105/250‑1 (MQ=255) cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGTGAGCGCAGAGAGGGGTTATC‑TTGCCTGACTGAACTGGCGATTTAGTTTTCTCGTACCCGGCGGTTAGTTTTTTCGGATGATGATATTTAAGCTCGGCTGG‑TTGGTTCCTTTTTGCCATCACCGCTG‑GGGGGTGGATTTATGAAAGCGTCAACGCTggggg > 2:685042/1‑247 (MQ=255) aTACCTATACCAGTCAGGATTCATTTCAGGATGCCTTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAt < 1:220772/250‑1 (MQ=255) aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAt < 1:317198/250‑1 (MQ=255) ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTTTGAAGTGAGCGCCGAGAGGGGTTATC‑TTTCCTGTCTGAACTGGCGGTTTAGTTTTCGCGTAACCCGCGTTTTGTTTTTGTGGGTGATGGTATTTTAGCTCGGCTGA‑TTGGTTCTTTTTCGCCCTCACCGCGGTGCGGGTGT‑TTTAGTAAAACGTCAAAGCTGGAGAt‑ttt > 2:781271/1‑250 (MQ=255) cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTCTTTTTTTGCCCTTGGAAGTGAGCGCAGAGAGGGGTTTTT‑TTTTCTGGCTTCACTGGCGATTAAGTTTTCGCTTACCCGGCGTTTTGTTTTTGTGGATTGTGTTATTTTAGGTTGGGTGT‑GTGGTTCATTTTTGTCCTCACCGCTG‑GCCGGTGGCTTAAGTAAATGCTCAAGGCTGGAGGTATTTtcttca > 2:954591/1‑250 (MQ=255) cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAg < 1:25363/250‑1 (MQ=255) ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGTGAGGGCCGAGAGGGGTTTTT‑TTGCCTGACTGAACTGGCGATTAAGTTTTCCCGTACCCGGCGGTTTGTTATTTCGGATGATTATATTTTAGCTCGGCTGG‑TTGGGTCCTTTTTGCCCTCCCCGCTG‑GACGGTGTATTTAGTTAAAAGTTCACGCTGGAGCT‑TTTTTCTCAGGGActc > 2:185625/1‑250 (MQ=255) agacattcaaGGGTGACATGTCGGGGATCTCGTGTGGGGCGGTCAGTTGTTCATTTTCTGCCATGAGAAGTGAGCGGAGAGAGGGAGTAGA‑TTGCCTGACTGAACAGGCGATTAAGTATCCGAGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCGCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAActcta < 2:200832/241‑2 (MQ=255) cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCttttt < 2:812273/250‑1 (MQ=255) aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGGAGAGAGGGGTTTTT‑TTTGCTGGCTTAACTGGCGGTTTAATTTCCGCGTAACCCGCGGTTTGTTATTTCGGGTGATGATATTGAAGCTCGGGTGA‑TTGGTTTCTTTTTGCCATCACCGCTG‑GGAGGGGTGTTAAGTAAAACGGCCACGGTGGGGGG‑TTTTCATCAGGGACTCTTTTTGTCAtt > 2:392830/1‑250 (MQ=255) aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCAtt < 1:364126/250‑1 (MQ=255) ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCGGAGAGGGGTTTTC‑TTTCCTTACTGTACTGGCGATTAAGTTTTCGCGTACCCCGCGTTTTGTTATTGCGGGTGGTGATATTTAAGCTCGGCTGG‑TTGGTTTATTTTCGCCATCCCCCCTG‑GACGGGGTATTTAGTAAAGGGTCAAAGCTGGGGAT‑TTTTTTTCAGGAACTCTTTTTTTCATTAAATgg > 2:997193/1‑250 (MQ=255) aTGTCGCGGATCTCGTTTTCGGGGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGgtg < 2:752115/250‑1 (MQ=255) tcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcga < 1:504012/250‑1 (MQ=255) gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgacc < 1:1091856/250‑1 (MQ=255) gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgacc < 1:804633/250‑1 (MQ=255) ggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGcgaccgaccga < 1:1050894/250‑1 (MQ=255) cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACt > 1:174481/1‑250 (MQ=255) cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAAc > 1:256841/1‑250 (MQ=255) tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAAt > 1:89560/1‑250 (MQ=255) tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAAt < 2:292575/250‑1 (MQ=255) ttttctttttctGCCATGAGAAGAGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGCTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATc > 2:1044697/1‑250 (MQ=255) | TGGATAAATAGCGCGCAGGGTGCAAGAGAACTTAACTTGCCTTCTGTTTATGGAGATGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATC‑TTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGA‑TTGGTTCATTGTCGCCATCACCGCTG‑GACGGTGTATTAAGTAAAGCGTCAACGCTGGAGAT‑TTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATC > NC_007779/4608775‑4609269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |