BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779	4,136,677	G→T	V191V (GTC→GTA)	frlD ←	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779	4,136,677	0	G	T	99.2%	479.5 / ‑4.2	125	V191V (GTC→GTA)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (49/75); total (49/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 127 total aligned reads displayed.

TGGCGTGAAGATGCTCACGCCAACGTTATACCTACCAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTGACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTGCGCCAGCCAGGC  >  NC_007779/4136433‑4136922

tGGCGTGAAGATGCTCACGCCAACGTTATACCTACCAGGCACCGTGTTACTGAATGTTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAAt                                                                                                                                                                                                                                                  <  1:892181/250‑1 (MQ=255)

    gttagggtgccgaCGCCAAGGTTATCCCTCCCAGGCACCGTGGTACTGATTGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACt                                                                                                                                                                                                                                              <  2:517762/238‑1 (MQ=255)

     tGAAGATGCTCCCGCCAACGTTATACCTGCCGGGCACCGTGGTCCTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTg                                                                                                                                                                                                                                             <  2:641184/250‑1 (MQ=255)

       aaGATGCCCACGCCAACGTTATACCTACCAGGCTCCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGtt                                                                                                                                                                                                                                           <  2:1307745/250‑1 (MQ=255)

                  gCCAACGTTATCCCTACCAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACTCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACg                                                                                                                                                                                                                                <  2:771760/250‑1 (MQ=255)

                   ccAACGTTATACCTACCAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACgg                                                                                                                                                                                                                               >  1:324479/1‑250 (MQ=255)

                   ccAACGTTATACCTACCAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACgg                                                                                                                                                                                                                               >  1:949734/1‑250 (MQ=255)

                    cAACTTTATACCTCCCAGGCACCGTGGTACGGAATGGTTTTCGCCGCGCCCGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACggg                                                                                                                                                                                                                              <  2:311877/250‑1 (MQ=255)

                          tatcCCTACCGGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGGCCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGcaacaa                                                                                                                                                                                                                        <  2:1021369/250‑1 (MQ=255)

                           ataCCTACCAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAAt                                                                                                                                                                                                                       <  2:609634/250‑1 (MQ=255)

                              cgtccccggCACTGTGGAACAGATTGGTTTTCGCCGGCCACGCCGTACCCTGCGCTATGACCAGCGGTAATGTCATCCCCTCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGc                                                                                                                                                                                                                    <  2:1307824/243‑1 (MQ=255)

                               ctcccAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCt                                                                                                                                                                                                                   <  2:1239150/247‑1 (MQ=255)

                                  ccAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTc                                                                                                                                                                                                                <  1:1055382/250‑1 (MQ=255)

                                     ggCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATc                                                                                                                                                                                                             >  1:1305194/1‑250 (MQ=255)

                                      cccccGTGGTCCTGATTGGTTTCCGCCGCGCTCGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCACCCCCGCAGCCCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCt                                                                                                                                                                                                            <  2:572689/247‑1 (MQ=255)

                                       cACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCtt                                                                                                                                                                                                           >  1:1030853/1‑250 (MQ=255)

                                            tGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGAc                                                                                                                                                                                                      >  1:338434/1‑250 (MQ=255)

                                               tACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCa                                                                                                                                                                                                   <  1:341409/250‑1 (MQ=255)

                                                 cTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGc                                                                                                                                                                                                 >  2:390747/1‑250 (MQ=255)

                                                 cTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGc                                                                                                                                                                                                 >  2:150427/1‑250 (MQ=255)

                                                  tGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCg                                                                                                                                                                                                >  1:755977/1‑250 (MQ=255)

                                                   gAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACg                                                                                                                                                                                                     <  1:639113/244‑1 (MQ=255)

                                                    aaTGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCAACCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGtt                                                                                                                                                                                              <  2:1288267/250‑1 (MQ=255)

                                                     ttGGTTTCCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGttt                                                                                                                                                                                             <  2:459764/249‑1 (MQ=255)

                                                      tGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTc                                                                                                                                                                                            <  1:972643/250‑1 (MQ=255)

                                                            cccccgcCCACGCCGTTCCCTGCGCTAACGCCGGCGGTAATGTCACCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGtcttc                                                                                                                                                                                      <  2:873071/247‑1 (MQ=255)

                                                              cccgcGCCCGCCTTTCCCTGCGCTATCGCCTGCGGTAATGTCAGCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTcttctt                                                                                                                                                                                    <  2:768399/249‑1 (MQ=255)

                                                                   gcACGCCGTTCCCGGCGATATCGCCTGCGGTAATGTAATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGGCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTtgcggt                                                                                                                                                                               <  2:733102/250‑1 (MQ=255)

                                                                    cACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAAAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTtgcggtg                                                                                                                                                                              <  1:710279/250‑1 (MQ=255)

                                                                      cGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGCACAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTGCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTtgcggtgcg                                                                                                                                                                            <  1:396641/250‑1 (MQ=255)

                                                                         cGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGag                                                                                                                                                                         <  1:293044/250‑1 (MQ=255)

                                                                         cGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGag                                                                                                                                                                         >  1:1160622/1‑250 (MQ=255)

                                                                            tCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGaggca                                                                                                                                                                      <  1:281847/250‑1 (MQ=255)

                                                                              ccTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGaggcaaa                                                                                                                                                                    >  1:229186/1‑250 (MQ=255)

                                                                               cTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGAGAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGaggcaaag                                                                                                                                                                   <  2:906836/250‑1 (MQ=255)

                                                                                 gcgcTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGaggcaaaggc                                                                                                                                                                 >  1:668484/1‑250 (MQ=255)

                                                                                     tATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCaaaa                                                                                                                                                             <  1:79457/250‑1 (MQ=255)

                                                                                     tATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCaaaa                                                                                                                                                             >  1:6573/1‑250 (MQ=255)

                                                                                     tATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCaaaa                                                                                                                                                             >  1:46212/1‑250 (MQ=255)

                                                                                       tCGCCTGCGGTAATGTCATCCCCGCCGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATc                                                                                                                                                           <  2:549457/250‑1 (MQ=255)

                                                                                        cGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCg                                                                                                                                                          <  2:232084/250‑1 (MQ=255)

                                                                                          ccTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCgag                                                                                                                                                        >  2:1358879/1‑250 (MQ=255)

                                                                                           cTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCgaga                                                                                                                                                       <  1:1296974/250‑1 (MQ=255)

                                                                                           cTGCGGTAATATAATCCCCGCAGCCCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCgaga                                                                                                                                                       <  2:172964/250‑1 (MQ=255)

                                                                                                gTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCgg                                                                                                                                                  <  2:178654/250‑1 (MQ=255)

                                                                                                       cATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAACATCGAGATGCGTCACCAgt                                                                                                                                           >  2:901069/1‑250 (MQ=255)

                                                                                                         tCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAgtgt                                                                                                                                         <  2:1787/250‑1 (MQ=255)

                                                                                                         cccccGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAgtgt                                                                                                                                         <  2:156727/249‑1 (MQ=255)

                                                                                                          ccccGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTc                                                                                                                                        >  1:1072686/1‑250 (MQ=255)

                                                                                                             cGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGc                                                                                                                                     <  2:1063636/250‑1 (MQ=255)

                                                                                                                aGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCag                                                                                                                                  >  1:758294/1‑250 (MQ=255)

                                                                                                                aGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCag                                                                                                                                  >  1:821534/1‑250 (MQ=255)

                                                                                                                 gACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCaga                                                                                                                                 <  1:541255/250‑1 (MQ=255)

                                                                                                                 gACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCaga                                                                                                                                 >  1:480061/1‑250 (MQ=255)

                                                                                                                       ccGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGc                                                                                                                           >  1:1297298/1‑250 (MQ=255)

                                                                                                                          cAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGt                                                                                                                        >  1:548293/1‑250 (MQ=255)

                                                                                                                                 aTCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACtt                                                                                                                 <  2:853724/250‑1 (MQ=255)

                                                                                                                                  tCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTg                                                                                                                >  1:398273/1‑250 (MQ=255)

                                                                                                                                    cGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCATTTGTc                                                                                                              >  2:138159/1‑250 (MQ=255)

                                                                                                                                    cGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTc                                                                                                              <  1:590585/250‑1 (MQ=255)

                                                                                                                                       cAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCa                                                                                                                                                                                                   <  2:118812/162‑1 (MQ=255)

                                                                                                                                       cAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCa                                                                                                                                                                                                   >  1:118812/1‑162 (MQ=255)

                                                                                                                                         aTGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGgaga                                                                                                         >  1:246834/1‑250 (MQ=255)

                                                                                                                                         aTGAACGAATCGCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGgaga                                                                                                         <  2:403963/250‑1 (MQ=255)

                                                                                                                                          tGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGaa                                                                                                        <  1:168174/250‑1 (MQ=255)

                                                                                                                                           gAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAg                                                                                                       >  1:1210203/1‑250 (MQ=255)

                                                                                                                                            aaCGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGt                                                                                                      >  1:222444/1‑250 (MQ=255)

                                                                                                                                                   ctcCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGc                                                                                               >  1:1324868/1‑250 (MQ=255)

                                                                                                                                                     ccGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCgg                                                                                             <  1:612629/250‑1 (MQ=255)

                                                                                                                                                         cACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAg                                                                                         >  1:893457/1‑250 (MQ=255)

                                                                                                                                                                     cGATAACCGTCACCGGTTCAGCAGCCTGACGCCAGAGCTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCATCGTTACAATCACGGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAGGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGc                                                                             <  2:743749/250‑1 (MQ=255)

                                                                                                                                                                      gATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCg                                                                            >  2:280844/1‑250 (MQ=255)

                                                                                                                                                                           ccGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAg                                                                       >  1:137470/1‑250 (MQ=255)

                                                                                                                                                                                      tCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATg                                                            >  2:632732/1‑250 (MQ=255)

                                                                                                                                                                                          gAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTc                                                        >  2:1104711/1‑250 (MQ=255)

                                                                                                                                                                                           aGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCt                                                       >  1:897864/1‑250 (MQ=255)

                                                                                                                                                                                              cTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTcc                                                    <  1:1358879/250‑1 (MQ=255)

                                                                                                                                                                                                         aCTGGGCGGCAACCCAAGCAATGCTGCCGGTTTCACCCAGCGTTACAATCCCTGGTCCTGCGCCACGGGCAACAATCGCTTTCAGATTCAGACGGAGCGTTTCGTCTTCTTGCGGTGCGGGGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCa                                         <  2:114520/250‑1 (MQ=255)

                                                                                                                                                                                                         aCTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCa                                         <  2:1315348/250‑1 (MQ=255)

                                                                                                                                                                                                                  cATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCcgc                                <  2:668484/250‑1 (MQ=255)

                                                                                                                                                                                                                    gcccATGCAATGGTGCCGTTTTCACCCAGCGTTGCAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAGTCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGt                              <  2:363541/249‑1 (MQ=255)

                                                                                                                                                                                                                     cccAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTg                             >  2:1003657/1‑250 (MQ=255)

                                                                                                                                                                                                                       cAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGATCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGca                           >  2:1089080/1‑250 (MQ=255)

                                                                                                                                                                                                                       cAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGca                           >  1:369351/1‑250 (MQ=255)

                                                                                                                                                                                                                       cAGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAAGCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGca                           <  2:65775/250‑1 (MQ=255)

                                                                                                                                                                                                                        aGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACACTCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGcac                          >  1:290216/1‑250 (MQ=255)

                                                                                                                                                                                                                        aGGCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGcac                          <  1:1094164/250‑1 (MQ=255)

                                                                                                                                                                                                                          gCAATGCTGCCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACaa                        <  2:893457/250‑1 (MQ=255)

                                                                                                                                                                                                                              tgctgcCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTc                    <  2:229186/250‑1 (MQ=255)

                                                                                                                                                                                                                                ctgcCGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCAt                  <  2:808328/250‑1 (MQ=255)

                                                                                                                                                                                                                                   ccGTTTTCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACt               <  2:1185784/250‑1 (MQ=255)

                                                                                                                                                                                                                                      ttttCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTgcg            >  1:1240176/1‑250 (MQ=255)

                                                                                                                                                                                                                                      ttttCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAAGGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTgcg            >  2:182588/1‑250 (MQ=255)

                                                                                                                                                                                                                                         tCACCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTGCgcca         <  2:519461/250‑1 (MQ=255)

                                                                                                                                                                                                                                           aCCCAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAACTCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTGCgccagc       <  2:804643/250‑1 (MQ=255)

                                                                                                                                                                                                                                            cccAGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTGCgccagcc      <  2:1269925/250‑1 (MQ=255)

                                                                                                                                                                                                                                               aGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTGCGCCAGCCAgg   <  2:1072686/250‑1 (MQ=255)

                                                                                                                                                                                                                                               aGCGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTGCGCCAGCCAgg   >  2:1365590/1‑250 (MQ=255)

                                                                                                                                                                                                                                                 cGTTACAATCACTGTTCCTGCGCCACGGGCTACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAAt                                                             <  1:1270406/190‑1 (MQ=255)

                                                                                                                                                                                                                                                 cGTTACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAAt                                                             >  2:1270406/1‑190 (MQ=255)

TGGCGTGAAGATGCTCACGCCAACGTTATACCTACCAGGCACCGTGGTACTGAATGGTTTTCGCCGCGCACGCCGTTCCCTGCGCTATCGCCTGCGGTAATGTCATCCCCGCAGACCAGCCGCAAAGGAATCCGGCAATGAACGAATCTCCGGCACCCATGGTGTCGATAACCGTCACCGGTTCAGGAGCCTGACGCCAGAACTGCGCGCCATCCCAGGCAATGCTGCCGTTTTCACCCAGCGTGACAATCACTGTTCCTGCGCCACGGGCAACAATCGCTTTCATCTTCAGACGCAGCGTTTCGTCTTCTTGCGGTGCGGAGGCAAAGGCAAAATCGAGATGCGGCACCAGTGTCTGCCAGAGCGGGCTGTCCCACTTGTCGGAGAAGTCGAAAGCGGTAAGTTTGCCCGCAGCGTGCAGCTGTGGGAATGCGTCTTCCGCATGTCCCCAGATTGCCGCGTGCACAATGTCATACTGCGCCAGCCAGGC  >  NC_007779/4136433‑4136922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`