breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 56 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0077793,895,0180TG33.9% 70.4 / 11.5 64L198F (TTA→TTCyiaN2,3‑diketo‑L‑gulonate transporter large permease YiaN
*NC_0077792,902,3480CA33.0% 233.3 / 59.4 106intergenic (+318/+1055)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077791,097,5800CT31.1% 75.3 / 48.7 61intergenic (+329/+45)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077794,291,3090TA30.4% 185.2 / 44.5 92intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NC_0077792,004,0550AC29.4% 229.6 / 16.9 102intergenic (‑129/+192)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_007779658,0980AC27.6% 193.9 / 22.7 88V88G (GTG→GGG) crcBfluoride efflux transporter CrcB
*NC_0077793,401,3140TC26.6% 285.8 / 40.1 128D625G (GAT→GGT) csrDRNase E specificity factor CsrD
*NC_0077792,902,3710GA26.6% 239.3 / 61.3 113intergenic (+341/+1032)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_0077793,974,7130GT26.5% 299.2 / 33.4 123A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NC_0077794,609,0240CT25.7% 211.1 / 25.2 101S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NC_0077791,437,4410GA25.3% 190.9 / 36.8 83intergenic (‑108/+33)uspF/ompNuniversal stress protein UspF/porin OmpN
*NC_0077792,004,0480GA25.0% 208.9 / 38.2 100intergenic (‑122/+199)Y75_RS10125/fliCRNA polymerase sigma factor FliA/H48 family flagellin FliC
*NC_0077791,826,7700GA24.7% 203.1 / 26.0 85intergenic (‑119/+84)ves/spyenvironmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
*NC_0077791,097,5400CA24.6% 148.5 / 25.0 57intergenic (+289/+85)Y75_RS05375/Y75_RS05385hypothetical protein/RtT sRNA
*NC_0077792,902,3670CA24.6% 355.9 / 39.0 114intergenic (+337/+1036)Y75_RS14470/queEkinase/7‑carboxy‑7‑deazaguanine synthase QueE
*NC_00777929,4960TA24.5% 368.3 / 50.6 143intergenic (+301/‑155)dapB/Y75_RS001654‑hydroxy‑tetrahydrodipicolinate reductase/glutamine‑hydrolyzing carbamoyl‑phosphate synthase small subunit
*NC_0077791,515,7090AG24.2% 205.3 / 25.0 100G55G (GGA→GGGY75_RS07620ABC transporter permease
*NC_0077792,391,5320GA23.9% 157.0 / 29.8 72intergenic (+31/‑72)Y75_RS11940/Y75_RS11945M28 family peptidase/YfbM family protein
*NC_0077791,068,5680TG23.8% 317.0 / 13.2 130G22G (GGT→GGGY75_RS05245general stress protein
*NC_0077791,437,4390CA23.8% 239.0 / 23.7 84intergenic (‑106/+35)uspF/ompNuniversal stress protein UspF/porin OmpN

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 381401 =NA (NA)70 (0.490) 61/492 4.3 NA coding (512/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381812 NA (NA)pseudogene (174/174 nt) Y75_RS23175 acetyltransferase
* ? NC_007779 = 4547370137 (0.960)57 (0.410) 56/478 4.7 33.9% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 = 4547666 90 (0.650)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 4547362 =134 (0.940)35 (0.250) 33/478 9.5 24.0% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NC_007779 4547676 = 93 (0.680)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NC_007779 = 294612342 (0.290)5 (0.050) 5/330 17.4 12.5% intergenic (+4/‑30) Y75_RS14670/Y75_RS14675 tRNA‑Met/tRNA‑Met
?NC_007779 2946344 = 42 (0.440)intergenic (+5/+69) Y75_RS14680/Y75_RS14685 tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase AmiC
* ? NC_007779 1413613 =151 (1.060)7 (0.050) 7/444 19.8 4.9% coding (50/936 nt) ttcA tRNA 2‑thiocytidine(32) synthetase TtcA
?NC_007779 = 1436672 135 (1.060)intergenic (+39/+227) Y75_RS24865/uspF hypothetical protein/universal stress protein UspF
* ? NC_007779 3541999 =125 (0.880)12 (0.090)
+23 bp
5/450 21.6 8.9% intergenic (+41/‑410) rhaA/rhaD L‑rhamnose isomerase/rhamnulose‑1‑phosphate aldolase
?NC_007779 = 3542343 145 (1.020)intergenic (+385/‑66) rhaA/rhaD L‑rhamnose isomerase/rhamnulose‑1‑phosphate aldolase