| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,390,235 | T→C | 50.7% | I44T (ATC→ACC) | hfq → | global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,390,235 | 0 | T | C | 50.7% | ‑4.6 / 15.1 | 16 | I44T (ATC→ACC) | hfq | global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication |
| Reads supporting (aligned to +/- strand): ref base T (3/5); new base C (4/4); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01 | |||||||||||
GAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAG > CP009273/4390094‑4390346 | gAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc > 1:228778/1‑144 (MQ=255) aaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGCCTTTTGATCAGTTCGTGACCCTGt > 1:249761/1‑144 (MQ=255) agagaATGGCTAAGGGGCCAGTTTGACAAGATCCGTTCCTGACCGCACTGCGTCGGGAACGTGTTCCAGGTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGACCCTGTTGa < 1:94554/144‑1 (MQ=255) tttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATGTGGTGAATGGTGTTAAGCTGAAAGGGCAAATCGCGTGTTTTGATCAGTTAGTGACCCTGTTGAAAAACACTGGCAGC‑CAGATGGt > 1:44753/1‑144 (MQ=255) tttACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTAATACAGATTCTAATTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGACCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACa > 2:3816/1‑149 (MQ=255) ttACAAGATCCGTTCCTGAACGCACTGCGTCGTGAACGTGTTCCAGTTTCTATATATTTGATGAAAGATATTAAGCTGCAAGGGCAAAACGAGTCTTTTGATCAATTCGTGACCCTGTTGAAAAACACGCGCAGC‑CAGATGGTATACaa > 2:123517/1‑149 (MQ=255) aCAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGGTTa > 1:6833/1‑144 (MQ=255) cTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGACCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTAc < 2:105793/149‑1 (MQ=255) gAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAGTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGCTTTCTACtg > 2:169265/1‑149 (MQ=255) cttcGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGACCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTACtgtt < 1:151868/141‑1 (MQ=255) cGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc < 2:231317/149‑1 (MQ=255) cAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcat < 2:188834/149‑1 (MQ=255) gtctCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatca < 2:250534/146‑1 (MQ=255) tttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTCTGATCAGTTCGTGATCCTGTTATGCTGGGCTGGCTGCTGGGATTGTTT‑CTAGCTCGCGATTTCTATTGTTGTCCCGTCTCTCCCGGTTTCTCATCACAGTaac < 2:124312/149‑1 (MQ=255) aaTGGCATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGCGACCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGcc < 1:61769/144‑1 (MQ=255) cTGCAAGGGCAAATCGAGTCTTTTGATCAGTCCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTATTGTTGTCTTGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagcag < 2:7541/149‑1 (MQ=255) | GAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGC‑CAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAG > CP009273/4390094‑4390346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |