| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 982,565 | T→A | 100% | intergenic (‑127/+476) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 982,565 | 0 | T | A | 72.7% | 11.4 / 3.0 | 11 | intergenic (‑127/+476) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base A (3/5); total (3/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01 | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
TGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACAC > CP009273/982426‑982670 | tGCGCTTCATCAGTATTTATTACCCTCTTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACaaa < 1:51726/144‑1 (MQ=255) aCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTTTGCATGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGc < 2:101452/149‑1 (MQ=255) aTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTATCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaa > 2:2790/1‑149 (MQ=255) gTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGGCTGCAGGCATCTTTTCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagat < 2:138902/149‑1 (MQ=255) gCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACTGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaaaa < 2:72378/149‑1 (MQ=255) ccGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAg < 1:248563/144‑1 (MQ=255) tgttGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGt < 1:2469/141‑1 (MQ=255) tCAATAAAAATTTACGGAACTATTGATGAGGGTTTGATGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGt > 2:210237/1‑149 (MQ=255) aCGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCAGCTTTCCATACAAACTAACGTTTCGCTACCGTTAAAGTGCTACAAAGATAAAGATTTTGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTa < 1:75322/144‑1 (MQ=255) aaCTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTTATAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACttt < 2:64611/149‑1 (MQ=255) atgaGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAAATAACAATTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAacac > 2:215671/1‑149 (MQ=255) | TGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACAC > CP009273/982426‑982670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |