| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,353 | A→G | 100% | intergenic (‑86/+135) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,353 | 0 | A | G | 71.4% | 16.0 / 2.7 | 14 | intergenic (‑86/+135) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base G (5/5); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
| Rejected as polymorphism: Not supported by required number of reads on both strands. | |||||||||||
CAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGT > CP009273/2420232‑2420492 | cAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAAAATCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTCGACGTattatt > 2:248583/1‑149 (MQ=255) gagatCGATAGCACCAGTTTTTGCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCa < 1:198676/144‑1 (MQ=255) tGTCCTCAACGGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATCTCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAAt < 1:141802/144‑1 (MQ=255) gTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTCTTTTTCATTAAAAAATCTTAAGTATGCATTTCAATg > 1:62033/1‑144 (MQ=255) cAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcacca > 1:192809/1‑144 (MQ=255) cAACTGACAGTCTTTTTATAGGGAATATTTACAGGCATGTTACACTTAAAGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGaacca > 1:105999/1‑144 (MQ=255) cTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTATACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTATACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGa < 1:75070/144‑1 (MQ=255) ttAAAGGGAATATTTACAGGTGTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTTATTAAATATTTTTTTGTTTGTTTTTCAATGCACCACGATAGTGCAACAAACCTgg < 2:65144/149‑1 (MQ=255) ggTTTGTTACACTTATCGTGCCATAAAATTGACTATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatgat < 1:119119/144‑1 (MQ=255) tttGCTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCAtgatga < 1:221275/144‑1 (MQ=255) tAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACAACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAg > 2:186870/1‑149 (MQ=255) aaTTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGTACTATGATGATCATTCCATCAGGTACAGCTTCCCAGCGa < 2:65125/149‑1 (MQ=255) tCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGt > 2:274336/1‑149 (MQ=255) ggCAAAAAATCCCGCCCCGTGCTTCGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAACGCACCACGATAGTGCAACAAACCTGGCGTGCATCATGATGATCATTCCATCAGGTACGTCTTCCCAGCGACGTAGCAg < 1:47667/144‑1 (MQ=255) | CAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGT > CP009273/2420232‑2420492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |