| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,718,735 | G→T | 54.2% | P124Q (CCG→CAG) | kgtP ← | alpha‑ketoglutarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,718,735 | 0 | G | T | 54.2% | 4.4 / 10.1 | 17 | P124Q (CCG→CAG) | kgtP | alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base G (6/2); new base T (5/4); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGG > CP009273/2718602‑2718878 | cGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCCTCAACGGCAACTTCACCCATATAGGTGGCGCTGGTGCCATATTCCTCGCCAACAGATAATCCCTGCAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGt < 1:102319/144‑1 (MQ=255) cTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTAGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCAt > 2:246746/1‑149 (MQ=255) gCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTTCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACATATAGTTTCATAACCt > 1:151862/1‑144 (MQ=255) catAAAAACCTTTCCCCCCTTCAACGGCAACTTCACTCATATAGTTGGCGCTGGTGCCATATTCTCCGCCAACATATAATCCCTGAAATAAACGAGCTAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTg < 1:115395/142‑1 (MQ=255) gTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCGTAGTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGaggc > 2:48254/1‑149 (MQ=255) ttGCGCCCTTCAACGGCAACTTCACTCATATAGGTGACGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCCGGCGATAAc > 2:44096/1‑149 (MQ=255) cTTCAACGGCAACTTCACTCATATAGGCGGCGCTGCTGCCATATCCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCCTAACCTGGGAGGCAGGCGATAACCa < 1:94598/144‑1 (MQ=255) aCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGCTCCGaaa > 1:64466/1‑144 (MQ=255) ggTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCACACGTAACTATAGTTTCATAACCTGGGAGGCCGGCGATAACCAGCGACCCGAAAAACATCATacacac > 1:61308/1‑144 (MQ=255) gaccgggTGCCATATTCTCCGCCAACAGATAATCCCGGAAATAACCGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCCATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt < 1:216303/139‑1 (MQ=255) ggTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGCAACACATCATACACACCGCTACCAg > 1:212844/1‑144 (MQ=255) cgTGCCATATTCTCGGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGGTCCGAAACACATCATACACACCGATAACAg > 1:143793/2‑144 (MQ=255) gATAATCCCTGAAATAAACGAGCGCGCAGCAATAATGCCGGAGCCCACATACCTATAGATTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATc > 2:54404/1‑149 (MQ=255) aaTCAACGAGCGAGAAGCAATAATGCCTGAGACCACGTACCTATAGTTTCATAACCTGGGAGGCAGGAGATAACAAGCGATCCGAAACACATCATACACCCCGCTACCAGCACAGGTTTTTTGCGACGAGCTTTAACGGCTCTg > 1:61938/1‑144 (MQ=255) aatGCCGGAGCCCACGTACCNANAGTNTCATACCCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGATTATGTTTATCGGTTATGCGGCTAAATAGCCAACCGCCTATTgg < 2:134592/149‑1 (MQ=255) atGCCTGGGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACTTCATAAACATCGATAAATGCATTGATTTTTTGTGTCTTTGTTTTTCGGCTATGCGGCCAAATAGCCAACCGCCTATTggg < 2:52857/149‑1 (MQ=255) atGCCTGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATCCGGCCAAATAGCCAACCGCCTATTggg > 2:82572/1‑149 (MQ=255) | CGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGCCAAATAGCCAACCGCCTATTGGG > CP009273/2718602‑2718878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |