Predicted mutation
evidence seq id position mutation annotation gene description
JC JC CP009273 2,400,257 IS5 (+) +4 bp intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 2282398 =NA (NA)35 (0.860) 33/290 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 2400257 = 0 (0.000)intergenic (‑137/‑783) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
* ? CP009273 = 2283592NA (NA)58 (1.420) 39/290 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?CP009273 = 2400260 0 (0.000)intergenic (‑140/‑780) lrhA/alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2

TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC  >  CP009273/2282257‑2282537
                                                                                                                                              |                                                                                                                                           
ttttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaagg                                                                                                                                            <  1:518287/143‑1 (MQ=255)
 tttATATGCTGTTCTCCGCCAGGATTGATTTTCGTCTTATTGGCGGTCCCATAAACCAGCTGGATATGTTTTTCGAGGACAACACCATCAACTGTCTCACCATGATCAACAGTTGATGACAATGCTATGGGGGAATAGaagga                                                                                                                                           >  1:583388/1‑143 (MQ=255)
     tatGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCCGCTGGATATCTTTTTCCAGGACAACACCATCCACTGTCTCACCATACTCAACAGTTGCTGCCAATGCTATGGGTGAATATAAGGAAGGt                                                                                                                                       >  1:16195/1‑143 (MQ=255)
       tGCTTTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATaa                                                                                                                               <  2:317695/149‑1 (MQ=255)
                 cGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCGTCAACTGTCTCACCATTCTCGACAGTTGATGCCAATGCTATGGGGTGTAATAAGTAAGGTGCGAATAAGCGGGGAAAt                                                                                                                     >  2:147485/1‑149 (MQ=255)
                    cAGGATTGATTTTCGCATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGa                                                                                                                        <  1:164868/143‑1 (MQ=255)
                      ggATTGATTTTCATATAAATAGCCTGCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                      <  1:131988/143‑1 (MQ=255)
                      ggAGTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                      <  1:429203/143‑1 (MQ=255)
                          tgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGCATAAGCGGGGAAATTCTTCTCgg                                                                                                            <  2:390285/149‑1 (MQ=255)
                              tttCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGGATAAGCGGGGAAATTCTtctc                                                                                                              >  1:328152/1‑143 (MQ=255)
                                        ttGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCa                                                                                                    <  1:510350/143‑1 (MQ=255)
                                                 ccATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttca                                                                                           <  1:32528/143‑1 (MQ=255)
                                                             tGGGTATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTATAGGCTGACTAAGtcatttcatgaaatgactga                                                                               <  1:154969/143‑12 (MQ=37)
                                                             tGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGCATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGtt                                                                               >  1:587605/1‑143 (MQ=255)
                                                                   tCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCGGTCATCTCATTTCTTCATGTTTGAGCCGAtttt                                                                   >  2:51564/1‑149 (MQ=255)
                                                                        ttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                              <  2:18403/149‑1 (MQ=255)
                                                                           cAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                 >  1:375367/1‑143 (MQ=255)
                                                                              gACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTATCCCGTag                                                        >  2:364232/1‑148 (MQ=255)
                                                                                    acCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt                                                  <  2:367475/149‑1 (MQ=255)
                                                                                               tctcACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAt                                             >  1:633830/1‑143 (MQ=255)
                                                                                                       tACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAc                               >  2:3696/1‑149 (MQ=255)
                                                                                                       tACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCACGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGCATCAGCCTAt                                     >  1:427201/1‑143 (MQ=255)
                                                                                                          tCAACAGTTGATGCCAATGCTATGGGTGAATACAACCACCGCGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACCTGCCTTGAATCAGCCTATTTa                                  >  1:109608/1‑143 (MQ=17)
                                                                                                            aaCAGTTGATGCCAATGCTATGGGTGATAACACGGAAGGTGCGAATAATCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTCAATTAGCCTATTTAGa                                <  1:133847/143‑1 (MQ=14)
                                                                                                            aaCAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGa                                <  1:463158/143‑1 (MQ=255)
                                                                                                                gTTGCTGCCAATGCTATGGGTGAATATAAGGCAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                      <  2:472851/149‑1 (MQ=255)
                                                                                                                 ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCAGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTTTTCg                     >  2:263311/1‑149 (MQ=255)
                                                                                                                      gCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCGTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAtt                >  2:482937/1‑149 (MQ=255)
                                                                                                                        cAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGAGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCACGTCAATGCCTTGAATCAGCCTCTTTATACCGTTTCTTCGc                    >  1:245415/1‑143 (MQ=14)
                                                                                                                         aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTaa             >  2:644357/1‑149 (MQ=255)
                                                                                                                             cTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg         <  2:585313/149‑1 (MQ=40)
                                                                                                                              tATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTa              >  1:202684/1‑143 (MQ=39)
                                                                                                                                   ggTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATc    <  2:641898/149‑1 (MQ=35)
                                                                                                                                     tGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTTTccc  >  2:199293/1‑149 (MQ=21)
                                                                                                                                         tataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATc    <  1:263582/143‑1 (MQ=31)
                                                                                                                                              |                                                                                                                                           
TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCC  >  CP009273/2282257‑2282537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.