Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	982,565	T→A	intergenic (‑127/+476)	ompF ← / ← asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	982,565	0	T	A	100.0%	89.4 / NA	31	intergenic (‑127/+476)	ompF/asnS	outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/12);  total (19/12)

TCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAAT  >  CP009273/982435‑982710                                                                                                                                   |                                                                                                                                                  tcatTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTa                                                                                                                                 >  2:629855/1‑149 (MQ=255)    ttatttatTACCCTCCTGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCg                                                                                                                                    <  1:108560/143‑1 (MQ=255)    ttatttatTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCg                                                                                                                              >  2:581778/1‑149 (MQ=255)     tatttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCATGAAGTTTTGTCGATAAAAATTTCCGGAACTATTGATGCGCGTTTGGTGTCATTATGTGTCCGCAGGACTCTTTCCATACAACCTGACGTTTCGc                                                                                                                                   >  1:106236/1‑143 (MQ=255)         tattgCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGaaa                                                                                                                         <  2:384566/149‑1 (MQ=255)               ccTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGaaa                                                                                                                         >  1:559160/1‑143 (MQ=255)                          tttttATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCTATACAAACTAACGTTTCGCTACCGTGAAAGTGCTTCaaag                                                                                                              >  1:50475/1‑143 (MQ=255)                              tATGACACCCGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACaaagataa                                                                                                          <  1:400174/143‑1 (MQ=255)                                 gACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGt                                                                                                 >  2:185771/1‑149 (MQ=255)                                  acacCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGGACTATTGATGAGGGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTTGtt                                                                                                >  2:365991/1‑149 (MQ=255)                                            cTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCaa                                                                                            >  1:191224/1‑143 (MQ=255)                                                 gacAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGa                                                                                       >  1:322932/3‑143 (MQ=255)                                                     aTAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGaaaaa                                                                                   >  1:262795/1‑143 (MQ=255)                                                               taaaTAAAAATTTACGGAACTATTGAGTATAGTTTGGTGTCTTTATGTGTCCGCAGGCATCTTTCCATACAAACTAACGTTTCGCGACCGTGAAAGTGCGACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAAcc                                                                         <  1:188313/141‑1 (MQ=255)                                                                 aaTAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTATGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCGACAAAGATAAAAATTTGGTTTCAAAAAGAAAAAATATGTAACaaa                                                                       >  1:399640/1‑143 (MQ=255)                                                                                  cTATTGATGAGCTTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCTATAAAATCTAACGTTTCGCTACCGTGAAAGGGCTACAAAGATAAAGATTTGGTTTCACAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTtgt                                                <  2:85494/149‑1 (MQ=255)                                                                                               tttGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTAAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACAcc                                         <  1:576277/143‑1 (MQ=255)                                                                                                 tGGTGTCTTTATGTGGCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGATACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTACCCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGt                                       <  1:396732/143‑1 (MQ=255)                                                                                                  ggTGTCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATAttt                                <  2:612018/149‑1 (MQ=255)                                                                                                    tgtCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTcc                              >  2:364355/1‑149 (MQ=255)                                                                                                      tCTTTATGTGTCTGCAGGCATCTTTCCATACAAGCTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGCACACCGTCATATTTCCAt                            >  2:451238/1‑149 (MQ=255)                                                                                                      tCTTTATGTGTCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCAt                            <  2:361905/149‑1 (MQ=255)                                                                                                             gtgtCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAATATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATa                           >  1:406448/1‑143 (MQ=255)                                                                                                              tgtCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGt                    >  2:111645/1‑149 (MQ=255)                                                                                                                tCTGCAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATagag                        >  1:90268/1‑143 (MQ=255)                                                                                                                    cAGGCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAATATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTgatgat              <  2:56082/149‑1 (MQ=255)                                                                                                                       gCATCTTTCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATAtt           >  2:649588/1‑149 (MQ=255)                                                                                                                           cTTTCCATACAAACAAACGTTTCCCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACa       <  2:236767/149‑1 (MQ=255)                                                                                                                            tttCCATACAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAAATTAAGGAACTTTGTGAACACCGTCATATGTCCATAGAGACATGATGATATTTACAg      >  2:627316/1‑149 (MQ=255)                                                                                                                               ccATACAAACTAACGTTTCGCTACCGTGAAAGTGCCACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTa         >  1:381942/1‑143 (MQ=255)                                                                                                                                cATACAAACTAACGTTTCGCTTCCGTGAAAGTGCTACAAAGATAATGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAATGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAAt  <  2:13955/149‑1 (MQ=255)                                                                                                                                   |                                                                                                                                                  TCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATCTTTCCATTCAAACTAACGTTTCGCTACCGTGAAAGTGCTACAAAGATAAAGATTTGGTTTCAAAAAGAAAAAATATGTAACCAAAAGTAAAATTTAAGGAACTTTGTGAACACCGTCATATTTCCATAGAGACGTGATGATATTTACAGCAAT  >  CP009273/982435‑982710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑