New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	2269338 =	1 (0.020)	55 (1.190)	45/290	0.1	98.2%	coding (587/1026 nt)	yejE	microcin C transporter YejABEF, permease subunit; ABC family
	?	CP009273	= 3577555	NA (NA)					intergenic (+15/‑1)	insB1/yrhA	IS1 transposase B/pseudogene, interrupted by IS1E

CTGACGCCAAGCGCCTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2269482‑2269338 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGC  <  CP009273/3577555‑3577417                                                                                                                                                                                                                                                                                              CTGACGCCAAGCGCCTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTA                                                                                                                                         >  1:725402/1‑149     CGCCACGCGCCTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGA                                                                                                                                     <  1:299122/149‑1       CCAAGCGCCTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACT                                                                                                                                   >  1:196042/1‑149        CAAGCGCCTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCTGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAA                                                                                                                                        <  2:229875/143‑1        CAAGCGCCTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAA                                                                                                                                        <  2:431348/143‑1               CTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGCATCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTg                                                                                                                           >  1:480160/1‑148                 GTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATT                                                                                                                         <  1:661001/149‑1                 GTGCCGCACGAATGTAGTCGAAATTACGAGTACGCAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAA                                                                                                                               <  2:476916/143‑1                 GTGCCGCACGAATGTAGTCGAAATGACGAGTACGTAAAGACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAA                                                                                                                               <  2:389993/143‑1                     CGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATA                                                                                                                     <  1:402529/149‑1                     CGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATA                                                                                                                     <  1:26017/149‑1                     CGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATACAGCCAAACAAGACAGTAATGGCCAGCAGCCACCAGAAGTTAGGCTGTACGACCCTGGGGAAATGACTCCAACTTA                                                                                                                           >  2:78516/1‑143                                 GTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTT                                                                                                         <  1:51701/149‑1                                  cCGAAATTACGATGACGTAAAAACGCCGCCCGCACCCCGCCGACCAGACTCATCCAGCTAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTT                                                                                                              <  2:540357/142‑1                                  TCGAAATTACGAGTACGTAAAATCTCCGCCCGCACCACGCCGACCAGACTCACCCAGGCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTCGGCGGTACGACGCTGGGGTAATGTCTCCAACTTATTCATAGTGTTTTATGTTC                                                                                                        <  1:500769/149‑1                                    GAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAG                                                                                                      <  1:505649/149‑1                                    GAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTAT                                                                                                            >  2:37669/1‑143                                        TTACGAGTACGTAAAAACCCCGCCCGCACCACGCCGACCAGACTCACCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAATTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTC                                                                                                        <  2:4636/143‑1                                            GAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCC                                                                                              >  1:301693/1‑149                                                ACGTAAAAGCTCCGCCCGAACCACGCCGACCAGACCCATCCAGGCCAACAAGACAGTAACTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATG                                                                                                <  2:651829/143‑1                                                    AAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGAGGACT                                                                                      >  1:719740/1‑149                                                      AAACCCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTT                                                                                    <  1:766175/149‑1                                                       AACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTTATAGTGTTCTATGTTCAGATAATGCCCGATG                                                                                         >  2:324302/1‑143                                                       AACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTG                                                                                   <  1:564064/149‑1                                                          TCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACT                                                                                      >  2:462217/1‑143                                                              CCCGCAACACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTACTGATAGTGTTTTATGTTCAGATACTGCCCGATGACTTTGT                                                                                  >  2:432873/1‑143                                                                     CACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAG                                                                           <  2:237030/143‑1                                                                              CAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGA                                                            <  1:86165/149‑1                                                                                      TCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACA                                                    <  1:164420/149‑1                                                                                      TCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTACAACTTATTGATCGTGAATTATGTTCAGATCATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGc                                                          >  2:423847/1‑142                                                                                             AAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTTCACGGATTTTGAGGACGGCAG                                                   >  2:209792/1‑143                                                                                               ACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCGACTCCACCGATTTTGAGAACGGCAGCGGCTTCC                                           >  1:414554/1‑149                                                                                               ACAAGACAGTAATTGCCAGCAGCCACCAAAAGGTAGGCTGGACGACGCTGCGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCC                                           <  1:106722/149‑1                                                                                                 AAGACCGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGAC                                               <  2:265635/143‑1                                                                                                       GTAACTACCAACAGCCACCAGAAGTTAGGCTGTACGACGCCGGCGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGC                                   <  1:299256/149‑1                                                                                                        TAACTGCCAGCAGCCACCAGAAGTGAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGTCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGCCAGCGACTTCCGTC                                        <  2:210571/143‑1                                                                                                         AATTGGCAGCAGCCACCAGGAGTTAGGCTGTACGACGCTGGGGTAATGTCTCCAACTTATTGATTGTGTTTTATGTTCAGTTCATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCC                                       <  2:340033/143‑1                                                                                                          atgGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGAGAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACACCGACTTCCGTCCC                                      <  2:430078/140‑1                                                                                                          ATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCC                                      >  2:601888/1‑143                                                                                                            TGCTAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTGATTGATAGTGTTTTATGTGCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAG                                    <  2:682935/143‑1                                                                                                             GCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGC                                   <  2:197175/143‑1                                                                                                               CAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGCCTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCCCCACCGATTTTGAGAAAGACAGCCACTTCCGTCCAACCCG                                 >  2:209884/1‑143                                                                                                                 GCAGCCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGT                         <  1:312313/149‑1                                                                                                                     CCACCAGAAGTTAGGCTTCACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAG                           <  2:554829/143‑1                                                                                                                     CCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTG                     >  1:322741/1‑149                                                                                                                     CCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCACCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTG                     >  1:567706/1‑149                                                                                                                     CCACCAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTCATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAAAGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTG                     >  1:536622/1‑149                                                                                                                         CAGAAGTTAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGACGACTTTGTCATGCAGCTCCACCGATTTTGCGAACGTCAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTC                 <  1:190112/149‑1                                                                                                                                TAGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTAAAAAAATGCGCGTTGACTTTGTCATGCAGCTCCACCGATTTTGAGAATGACTACTTCGTCCGTCCCAGCCGTGCCAGGTGGTACCCCAGATTCA          >  1:461349/1‑149                                                                                                                                 AGGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAG         <  1:447790/149‑1                                                                                                                                 AGGCCGTACGCCGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAG         <  1:64870/149‑1                                                                                                                                  GGCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGG        <  1:520208/149‑1                                                                                                                                   GCTGTACGACGCTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACCGCGAGTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGAT             >  2:334858/1‑143                                                                                                                                       TACGACGCTGGGGTAACGACTCCATCTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAG         <  2:205125/143‑1                                                                                                                                              CTGGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGC  >  2:224913/1‑143                                                                                                                                                                                                                                                                                              CTGACGCCAAGCGCCTGTGCCGCACGAATGTAGTCGAAATTACGAGTACGTAAAAACTCCGCCCGCACCACGCCGACCAGACTCATCCAGCCAAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2269482‑2269338 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGC  <  CP009273/3577555‑3577417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.