Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,054,744 G→C T224R (ACG→AGG)  nac ← nitrogen assimilation regulon transcriptional regulator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,054,7440GC100.0% 156.1 / NA 47T224R (ACG→AGG) nacnitrogen assimilation regulon transcriptional regulator; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/23);  total (24/23)

GTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCC  >  CP009273/2054604‑2054888
                                                                                                                                            |                                                                                                                                                
gTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATGCTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTg                                                                                                                                                >  2:555449/1‑143 (MQ=255)
    aTTTAATGACAACGGGGGCCTCATGGCAGGAGTGGTAATGCGTGACATCCACCCATTTACGGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCaa                                                                                                                                            <  2:61773/143‑1 (MQ=255)
          tGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCACTAGGCt                                                                                                                                      >  2:287104/1‑143 (MQ=255)
           gACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTc                                                                                                                                     <  2:415586/143‑1 (MQ=255)
            aCAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAGGCCTGGCAATAGACTCa                                                                                                                                    >  2:401013/1‑143 (MQ=255)
             cAAAGAGAGACTCATGGAGGGAGTGGTAAGGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCaa                                                                                                                                   <  2:335142/143‑1 (MQ=255)
              aaaGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCCACGCCCCTGCCGCGAACAATAGCTGCGACAAGCCTGCCAATAAGCTCAAt                                                                                                                                  >  2:308832/1‑143 (MQ=255)
                      aCTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCCGCGGTAAGACTGGCAATAGACTCCATTTCCCCaa                                                                                                                          >  2:291500/1‑143 (MQ=255)
                             gAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACttt                                                                                                                   >  2:531608/1‑143 (MQ=255)
                             gAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGt                                                                                                             <  1:86651/149‑1 (MQ=255)
                              aaGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACtttt                                                                                                                  >  2:708703/1‑143 (MQ=255)
                              aaGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACtttt                                                                                                                  <  2:381004/143‑1 (MQ=255)
                              aaGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACGCAATTTCACCAATAACtttt                                                                                                                  >  2:546034/1‑143 (MQ=255)
                                     ggTAATGCGTGATATCCACCCATTGACCGCGCCACACACCGCACGCGCGGCCGATTCGGGTAATACTGCAACGTCCATGCCGCTGTCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGa                                                                                                           <  2:555928/143‑1 (MQ=255)
                                     ggTAATGCGTGACACCCCCCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGa                                                                                                           <  2:311333/143‑1 (MQ=255)
                                      gTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAAACTCAATTTCACCAATAACTTTTGACGTGAg                                                                                                          >  2:270197/1‑143 (MQ=255)
                                               gACATCCACCCATTTACTGCGCCAAATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGCGTCTCCGTAg                                                                                                 <  2:121940/143‑1 (MQ=255)
                                               gACAGCCCCCCATTTACCGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCAGGCCGCTGGCAATCACTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGaaaa                                                                                           <  1:39021/149‑1 (MQ=255)
                                                aCATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAgg                                                                                                >  2:454841/1‑143 (MQ=255)
                                                aCATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGaaaaa                                                                                          <  1:713291/149‑1 (MQ=255)
                                                        ccATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAGTAACTTTCGCCGTGAGTCGCCGGAGGGAAAAAGc                                                                                        >  2:392077/1‑143 (MQ=255)
                                                         cctttACTGCGCCACATAACGCACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGcc                                                                                       <  2:108217/141‑1 (MQ=255)
                                                               cTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGa                                                                           <  1:593329/149‑1 (MQ=255)
                                                                  cgcCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCGATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGt                                                                        >  1:219396/1‑149 (MQ=255)
                                                                  cgcCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGACGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTCCCGTGAGTCGCCGTAGGGAAAAGCCTCATCAACACGAAgcc                                                                       >  1:611944/1‑147 (MQ=255)
                                                                      acaTAACGATCGCGCTGCCGATTCGGGTAATCCTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGTCTGGCAATAGACTCAATTTCACCAATCACTTTTGCCGTGAGTAGCCGTAGGGAAAAAGCCTCATCAACACGaa                                                                          <  2:231078/143‑1 (MQ=255)
                                                                      acaTAACGAACGCGCGGCCGATTCTGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTaa                                                                    >  1:401408/1‑149 (MQ=255)
                                                                      acaTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGCGTCGCCGTAGGGAAAAAGCCTCATCAACACGac                                                                          >  2:633631/1‑142 (MQ=255)
                                                                      acaTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGaa                                                                          <  2:587162/143‑1 (MQ=255)
                                                                      acaTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTaa                                                                    >  1:667335/1‑149 (MQ=255)
                                                                        atgacgaacgCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATa                                                                  <  1:414854/146‑1 (MQ=255)
                                                                           acgaacgCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCACGCGCTGCATTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGACTAATAGCa                                                               >  1:156058/1‑149 (MQ=255)
                                                                           acgaacgCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTa                                                                     >  2:5327/1‑143 (MQ=255)
                                                                            cgaacgGGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTaa                                                                    <  2:226443/143‑1 (MQ=255)
                                                                                     ggCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTAGACGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGt                                                           >  2:647301/1‑143 (MQ=255)
                                                                                       ccGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTaa                                                         >  2:682159/1‑143 (MQ=255)
                                                                                           ttCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCAc                                                     <  2:491975/143‑1 (MQ=255)
                                                                                               ggTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAGTAACGTCGGCCATAAGTCGCCTTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCTCTggg                                                 <  2:319522/143‑1 (MQ=255)
                                                                                                     aCTGCAACGCCCCTGCCGCTGCCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGaaa                                           <  2:607267/143‑1 (MQ=255)
                                                                                                        gCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAaggg                                        >  2:72924/1‑141 (MQ=255)
                                                                                                        gCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAagag                                        >  2:105468/1‑143 (MQ=255)
                                                                                                                 aTGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAGTAGACTCAATTTCACCAATAACTTTTGCCGTGGGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAAt                         >  1:508443/1‑149 (MQ=255)
                                                                                                                  tGCCGCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCCCCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATa                        <  1:454016/149‑1 (MQ=255)
                                                                                                                      gCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTACGCAATAGCAt                    <  1:504503/149‑1 (MQ=255)
                                                                                                                      gCTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTCGCGCaa                          >  2:81401/1‑143 (MQ=255)
                                                                                                                       cTGGCAATCGCTGCGGTAAGCCTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCAtt                   <  1:6386/149‑1 (MQ=255)
                                                                                                                                        aaGCCTGCCAACAGACCCAAAATCCCCAACAACTTTTGCCGTGAGTCGCCGTACGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACCGTAATCACTGGGGAGAAACACGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGcc  <  1:650638/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
GTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCC  >  CP009273/2054604‑2054888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: