Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,420,353 A→G 40.7% intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,420,3530AG40.7% 8.3 / 17.2 22intergenic (‑86/+135)hisJ/argThistidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (8/5);  new base G (3/6);  total (11/11)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAG  >  CP009273/2420221‑2420500
                                                                                                                                    |                                                                                                                                                   
cTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAACTGACAGGCTCTTTATAGGGAATATTTGCGGGTTTGGTACACTTATCGTGCCTTATCATTTACCCTCAAGGCAGGATATCCTGCCCCGTTa                                                                                                                                     >  1:285349/1‑149 (MQ=255)
  ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGa                                                                                                                                   >  1:18007/1‑149 (MQ=255)
   gagaAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAACTGACAGTCTTTTTATATGGAATATTTAGAGGCTTGTTACACTTATCGTGCCATCAAATTTACCATCAAGGCCAAATATACCGCTCCGGTAGAc                                                                                                                                  >  1:340670/1‑149 (MQ=255)
              aaCCAGAGATAGCGATATCACCATTTTTTTCATATTGTCCTCAACTGATAGTCTTTTTATAGGGTATATTTTCAGGTTTGTTACACTTAGCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTAttttt                                                                                                                       <  2:26020/149‑1 (MQ=255)
                                      ttttatCAAAATGTACTTAACTGAGAGTCTTTTTATAGGGAAGTTTTACAGGTGTGTTACGCTTATCTTGCCATAAAAGTGACCATCAAGGCATGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCAt                                                                                               <  1:145682/149‑1 (MQ=255)
                                                aTGTCCTCAACGGACAGTCTGTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcac                                                                                     <  1:115002/149‑1 (MQ=255)
                                                    ccTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACg                                                                                 >  1:194774/1‑149 (MQ=255)
                                                     cTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACgg                                                                                >  2:151188/1‑148 (MQ=255)
                                                       cAACTGAAAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATa                                                                              <  2:161987/149‑1 (MQ=255)
                                                                   tttttATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAcc                                                                  >  1:104846/1‑149 (MQ=255)
                                                                            ggAATATTTACGTGTTTGTTCCACTTTTCGTGCCATAAAATTGACCATCCAGGCAAGATATCCCGCCCCGTTAGACGTATGATTTTTCATTAAAAATTCTTAGGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa                                                         <  1:193241/149‑1 (MQ=255)
                                                                            ggAATATTTACAGGTTTGTTACAATTATCGTGTCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATAAGTTCTTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa                                                         <  2:208194/149‑1 (MQ=255)
                                                                                              ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGAGCATTCCAt                                       >  2:115769/1‑149 (MQ=255)
                                                                                              ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTACAACAAACCTGGCGTGCACCATGTTGATCATTCCAt                                       >  1:98900/1‑149 (MQ=255)
                                                                                                acacTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCa                                     <  1:80055/149‑1 (MQ=255)
                                                                                                             cATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAATGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTccc                        >  1:111831/1‑149 (MQ=255)
                                                                                                             cATAAAATTGACCATCAAGGCAAAATATCCCGTCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTccc                        <  2:209647/149‑1 (MQ=255)
                                                                                                              aTAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATAATCATTCCATCAGGTACAGCTTCCCa                       >  1:172809/1‑149 (MQ=255)
                                                                                                                aaaaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTCTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGACGCCCACTCCATCAGGTACAGCTTCCCAGc                     <  1:327790/149‑1 (MQ=255)
                                                                                                                          aTCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCac           >  1:158839/1‑148 (MQ=255)
                                                                                                                           tCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGt          <  1:14301/149‑1 (MQ=255)
                                                                                                                               ggCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCAcc      <  2:42875/149‑1 (MQ=255)
                                                                                                                                   aaaTATCCCGCCCCGTTAGACGTATTATTATTCATTAAAAATGCTTAAGTATGTATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCAGCAGTTTCAGCTTCCCGGGGACGTATCAGTCACCGTAg  >  1:196417/1‑149 (MQ=255)
                                                                                                                                    |                                                                                                                                                   
CTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAG  >  CP009273/2420221‑2420500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: