Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP009273 | 2,420,353 | A→G | 40.7% | intergenic (‑86/+135) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP009273 | 2,420,353 | 0 | A | G | 40.7% | 8.3 / 17.2 | 22 | intergenic (‑86/+135) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
Reads supporting (aligned to +/- strand): ref base A (8/5); new base G (3/6); total (11/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAG > CP009273/2420221‑2420500 | cTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAACTGACAGGCTCTTTATAGGGAATATTTGCGGGTTTGGTACACTTATCGTGCCTTATCATTTACCCTCAAGGCAGGATATCCTGCCCCGTTa > 1:285349/1‑149 (MQ=255) ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGa > 1:18007/1‑149 (MQ=255) gagaAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTTTTTTTTTTTTCAACTGACAGTCTTTTTATATGGAATATTTAGAGGCTTGTTACACTTATCGTGCCATCAAATTTACCATCAAGGCCAAATATACCGCTCCGGTAGAc > 1:340670/1‑149 (MQ=255) aaCCAGAGATAGCGATATCACCATTTTTTTCATATTGTCCTCAACTGATAGTCTTTTTATAGGGTATATTTTCAGGTTTGTTACACTTAGCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTAttttt < 2:26020/149‑1 (MQ=255) ttttatCAAAATGTACTTAACTGAGAGTCTTTTTATAGGGAAGTTTTACAGGTGTGTTACGCTTATCTTGCCATAAAAGTGACCATCAAGGCATGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCAt < 1:145682/149‑1 (MQ=255) aTGTCCTCAACGGACAGTCTGTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcac < 1:115002/149‑1 (MQ=255) ccTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACg > 1:194774/1‑149 (MQ=255) cTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACgg > 2:151188/1‑148 (MQ=255) cAACTGAAAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATa < 2:161987/149‑1 (MQ=255) tttttATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAcc > 1:104846/1‑149 (MQ=255) ggAATATTTACGTGTTTGTTCCACTTTTCGTGCCATAAAATTGACCATCCAGGCAAGATATCCCGCCCCGTTAGACGTATGATTTTTCATTAAAAATTCTTAGGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa < 1:193241/149‑1 (MQ=255) ggAATATTTACAGGTTTGTTACAATTATCGTGTCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATAAGTTCTTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa < 2:208194/149‑1 (MQ=255) ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGAGCATTCCAt > 2:115769/1‑149 (MQ=255) ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTACAACAAACCTGGCGTGCACCATGTTGATCATTCCAt > 1:98900/1‑149 (MQ=255) acacTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCa < 1:80055/149‑1 (MQ=255) cATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAATGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTccc > 1:111831/1‑149 (MQ=255) cATAAAATTGACCATCAAGGCAAAATATCCCGTCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTccc < 2:209647/149‑1 (MQ=255) aTAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATAATCATTCCATCAGGTACAGCTTCCCa > 1:172809/1‑149 (MQ=255) aaaaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTCTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGACGCCCACTCCATCAGGTACAGCTTCCCAGc < 1:327790/149‑1 (MQ=255) aTCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCac > 1:158839/1‑148 (MQ=255) tCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGt < 1:14301/149‑1 (MQ=255) ggCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCAcc < 2:42875/149‑1 (MQ=255) aaaTATCCCGCCCCGTTAGACGTATTATTATTCATTAAAAATGCTTAAGTATGTATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCAGCAGTTTCAGCTTCCCGGGGACGTATCAGTCACCGTAg > 1:196417/1‑149 (MQ=255) | CTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAG > CP009273/2420221‑2420500 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |