| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,420,394 | G→T | 36.6% | intergenic (‑127/+94) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,420,394 | 0 | G | T | 36.6% | 12.7 / 16.0 | 22 | intergenic (‑127/+94) | hisJ/argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (8/6); new base T (2/6); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01 | |||||||||||
TTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGT > CP009273/2420259‑2420537 | ttttatCAAAATGTACTTAACTGAGAGTCTTTTTATAGGGAAGTTTTACAGGTGTGTTACGCTTATCTTGCCATAAAAGTGACCATCAAGGCATGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCAt < 1:145682/149‑1 (MQ=255) aTGTCCTCAACGGACAGTCTGTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGcac < 1:115002/149‑1 (MQ=255) ccTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACg > 1:194774/1‑149 (MQ=255) cTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACgg > 2:151188/1‑148 (MQ=255) cAACTGAAAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATa < 2:161987/149‑1 (MQ=255) tttttATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAcc > 1:104846/1‑149 (MQ=255) ggAATATTTACGTGTTTGTTCCACTTTTCGTGCCATAAAATTGACCATCCAGGCAAGATATCCCGCCCCGTTAGACGTATGATTTTTCATTAAAAATTCTTAGGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa < 1:193241/149‑1 (MQ=255) ggAATATTTACAGGTTTGTTACAATTATCGTGTCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATAAGTTCTTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCa < 2:208194/149‑1 (MQ=255) ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGAGCATTCCAt > 2:115769/1‑149 (MQ=255) ttACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTACAACAAACCTGGCGTGCACCATGTTGATCATTCCAt > 1:98900/1‑149 (MQ=255) acacTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCa < 1:80055/149‑1 (MQ=255) cATAAAATTGACCATCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAATGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTccc > 1:111831/1‑149 (MQ=255) cATAAAATTGACCATCAAGGCAAAATATCCCGTCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTccc < 2:209647/149‑1 (MQ=255) aTAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATAATCATTCCATCAGGTACAGCTTCCCa > 1:172809/1‑149 (MQ=255) aaaaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTCTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGACGCCCACTCCATCAGGTACAGCTTCCCAGc < 1:327790/149‑1 (MQ=255) aTCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCac > 1:158839/1‑148 (MQ=255) tCAAGGCAAGATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGt < 1:14301/149‑1 (MQ=255) ggCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCAcc < 2:42875/149‑1 (MQ=255) aaaTATCCCGCCCCGTTAGACGTATTATTATTCATTAAAAATGCTTAAGTATGTATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCAGCAGTTTCAGCTTCCCGGGGACGTATCAGTCACCGTAg > 1:196417/1‑149 (MQ=255) cccGCCCCGTTAGACGTATTATTTTTCATTAAAAATTCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTa < 2:104846/149‑1 (MQ=255) ttCATAAAAAATGCTTAAGGAGGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATc < 1:289759/149‑1 (MQ=255) aaaaTGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGt > 1:194764/1‑149 (MQ=255) | TTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGACATTAAAGTCGAAATACTTTTTCGCCATCTTGTCGT > CP009273/2420259‑2420537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |