| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,641,079 | G→A | 17.2% | S1523L (TCG→TTG) | yfhM ← | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,641,079 | 0 | G | A | 17.2% | 45.1 / 2.7 | 23 | S1523L (TCG→TTG) | yfhM | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (11/8); new base A (2/2); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCT > CP009273/2640935‑2641223 | tGCCCCACGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTc < 1:276798/149‑1 (MQ=255) ggCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGc < 2:19494/149‑1 (MQ=255) gcTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGctct > 2:300062/1‑149 (MQ=255) aCCGTTCGCCAGATTCTGCTTTTCCAGTTCCATACCCGCAGGCAGCAGATCCACGACTAATACGCCCTTCACGCTGTTACTGGCTGTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTAc < 2:172051/149‑1 (MQ=255) cccGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACCCCAGCACCAGATCGCCGCTACGTAACGCGTCCAGCGATTTGCTCTTACCATCAGTAACAAGAATATGACGCTCGATTTgcag < 2:178775/149‑1 (MQ=255) gcaggcagCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACAAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGcac > 2:147568/1‑149 (MQ=255) gcaggcagCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACAAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGcac > 2:147558/1‑149 (MQ=255) caggcagCAGATCCACGACTAACGCAGCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGcaca < 2:287497/149‑1 (MQ=255) caggcagCAGATCCACGACTAACGCACCCGGCACTCTGTTACTGGCTGTTACCTGCAACCACGCCAGCACCAGATCGCCGCTCCGTAACGCGTCCAGCCATTTGCTCTTATCATCAGTCCCAAGAATATGACGCTCGATTTGCAGcaca < 1:174570/149‑1 (MQ=255) cagATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATCTGACGCTCGATTTGCAGCACATTGTTCg > 2:97989/1‑149 (MQ=255) aTCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCACAGCGCCGCTACGCAACGCGCCCACCGATTTGCTCCTACCATCCGTCCCACGAATGTGACCCCCGCCTTGCCGcacctccctcgccg > 1:285251/1‑138 (MQ=255) aTCCGGCACGCTGTTACTGGCTTGTACCTGCAACCACACCAGCACCAGATCGCCGCTACATAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtccggatt > 1:263910/1‑149 (MQ=255) cGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcg > 1:72696/1‑149 (MQ=255) cGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTAAGTAACGAGTCCAGCGATTTGCTCTTACCATCCGTACCAAGAAAATGAGGATGGATTCGCCGCATAGTGTTCACGGGTGAAGGtgcgggtcgcg > 1:228818/1‑149 (MQ=255) gCACGCTGTTACTTGCTTTTACCTGCAACCACACCAGCACCCGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGtgcggattgcgga < 1:72573/149‑1 (MQ=255) cGCTGTTACTCGCTTTTACACGCAACCACACAAGCACCAGAACGCCGCTACGTAACGAGTCCAACGATTTGCTCATAGCCTCTGTTACAAGAAGAGGACTCCCGATTTGCAACACATTGTTCGCCGGTAAAGGTGCTGATTGCGGATaa < 1:343689/149‑1 (MQ=255) cacaCCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATag > 2:135050/1‑148 (MQ=255) accagATCGCCGCTACGTAACAAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATc < 1:147558/149‑1 (MQ=255) accagATCGCCGCTACGTAACAAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATc < 1:147568/149‑1 (MQ=255) cagATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGc > 1:350869/1‑149 (MQ=255) gccgcTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCGAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGt > 1:306998/1‑149 (MQ=255) tAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCCCTGTTGCTCACCt > 2:175937/1‑149 (MQ=255) tAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGACGGTAAAGGTTCGTGTGGCGGATAACCGCTGGCATCCATACGCAGCCATAACGGCTGATCGCCACGGTTTCTCAccg > 1:305823/1‑148 (MQ=255) | TGCTCCAGGCTGGCGCTACCGTTCGCCAGATTCTGGTTTTCCAGTTCCAGACCCGCAGGCAGCAGATCCACGACTAACGCATCCGGCACGCTGTTACTGGCTTTTACCTGCAACCACACCAGCACCAGATCGCCGCTACGTAACGAGTCCAGCGATTTGCTCTTACCATCAGTACCAAGAATATGACGCTCGATTTGCAGCACATTGTTCGCCGGTAAAGGTGCGGATTGCGGATAACCGCTGGCATCCATACGCAACCATAACGGCTGATCGCCACTGTTGCTCACCT > CP009273/2640935‑2641223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |