| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,348,853 | A→G | 16.0% | K257R (AAA→AGA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,348,853 | 0 | A | G | 16.0% | 44.9 / 3.2 | 25 | K257R (AAA→AGA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (10/11); new base G (2/2); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01 | |||||||||||
GCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGT > CP009273/3348711‑3348989 | gCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCa > 1:83999/1‑149 (MQ=255) tGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAGATTCCAGCCACCGCtt > 2:112454/1‑149 (MQ=255) tGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGAGTGCGCGTACTTATAGATTCCAGACACCGCtt > 2:243559/1‑149 (MQ=255) accgGCGCGTCTCCCCTACCCCCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACTCCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCt < 1:128921/149‑1 (MQ=255) cacCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCAc > 2:268522/1‑149 (MQ=255) gcgcTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCCCGACGCCGCACCGTTCGTCa > 2:73561/1‑149 (MQ=255) ccctcgccgccggcGCAACCGCTCCGCGACCTCGCGCATAACGGTCAAATCAACCCCATCACCGGTAACCGCCAATGGGCGCCCGCGCCCACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCaa < 1:8704/144‑1 (MQ=255) aaCCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCAGCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACCCCCAACCCTTCGTCAACGAAACCGGCTCTGAc > 1:47498/1‑149 (MQ=255) cGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTcc > 2:46428/1‑149 (MQ=255) cGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAAAGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCCTATCCCTGACTTGCACGACGCCGCACCGTTCGTCAACGAAACCAGCTCTGACTCCAGTTc > 2:249009/1‑149 (MQ=255) tgaTCTGGCGCATAACGGTGAAATCCACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCATTTcta < 1:237861/147‑3 (MQ=255) ccaccTGGCGTACAACGGTGAACTCAACACCACCACCGGCAACCGCCACTGGGCTCGTGCGCGTACTTCTAAATGCCAGCCATCGCTTATCCCTGTCCTGCACGACGCCGCACCGTTCGTCAACGAAACCTGCTCTGACTCCAGTTCGa < 2:115274/145‑1 (MQ=255) tAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGc < 2:278655/149‑1 (MQ=255) tggAAATCGACCCCATCACCGGTAACCGCCAATGGGCGCGGGCGCGTACTTATAAATTCCAGACACCGCTTATCCCAGAAGCGCGGTATTCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCCGTTCCATGGATAACATGCTGGaa < 1:76605/147‑1 (MQ=255) gTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGaa < 2:177568/149‑1 (MQ=255) aaaTCAACACCATCACCGGGAACCGCCAATGGGCGCGTTCGCGTACTTATAAATACCAGTGATCCCGTACCCCTGCCCTGAACGACGCCGCACCGTCCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAActg < 2:83999/149‑1 (MQ=255) aTCAACCCCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGCCTCCAGTTCGATGGATAACATGCTGGAActgct < 2:55738/149‑1 (MQ=255) aaCACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAGATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATTGATAACATGCTGGAActgctgct < 1:243559/149‑1 (MQ=255) aaCACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAGATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAActgctgct < 1:112454/149‑1 (MQ=255) ccGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGGTGGata > 1:23183/1‑149 (MQ=255) gCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATatc > 1:92282/1‑149 (MQ=255) gcgcgtgcgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAATTGCTGCTGGCAGGCGGGATGGATATCATCcgcgg > 1:120400/1‑148 (MQ=255) gcgcgtgcgcgtACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCgcgc > 1:257648/1‑149 (MQ=255) ctcgtACTAATGAATTCCAGACACCGCTTATCCCGGACCTGCACGCCGCCGCACCGTTCGTCAACGACACCGGCTCTGGCTCCAGTTCGATGGATAACATGCTGTAACTGCGGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGt < 1:335266/147‑1 (MQ=255) cgcgtACTTATAAATTCCAGGCACCGCTTATCCCTGAGCGGCACGACGCCGCACCGTGCGTCAACGGAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTAGAACTGCTGCTGGCAGGCGGGATGGATATCAGCCGCGCCATGCGt < 1:249009/149‑1 (MQ=255) | GCCATTTGCCTGTTCCACCAGCGCTTCTCCACTAACACCGTACCGCGCTGGCCGCTGGCGCAACCGTTCCGCTATCTGGCGCATAACGGTGAAATCAACACCATCACCGGTAACCGCCAATGGGCGCGTGCGCGTACTTATAAATTCCAGACACCGCTTATCCCTGACCTGCACGACGCCGCACCGTTCGTCAACGAAACCGGCTCTGACTCCAGTTCGATGGATAACATGCTGGAACTGCTGCTGGCAGGCGGGATGGATATCATCCGCGCCATGCGT > CP009273/3348711‑3348989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |