| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,115,408 | T→C | 13.3% | E444G (GAA→GGA) | priA ← | Primosome factor n' (replication factor Y) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,115,408 | 0 | T | C | 13.3% | 69.8 / 4.2 | 30 | E444G (GAA→GGA) | priA | Primosome factor n' (replication factor Y) |
| Reads supporting (aligned to +/- strand): ref base T (13/13); new base C (2/2); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01 | |||||||||||
GGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCAGACCTGCCTGCACCTTCTGACCTTT > CP009273/4115266‑4115556 | ggggCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCaa < 1:354748/146‑1 (MQ=255) ggggACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGt > 2:319737/1‑149 (MQ=255) aCCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGca < 2:295094/149‑1 (MQ=255) cGTGGAACCGCAGGAGGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGGGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTTGGCATTCGGCAATCCAGCCACAGTCGTGGCGCAGCAGTg > 1:166990/1‑149 (MQ=255) ggAACCGCAGGAAGGGGACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAg < 2:51954/149‑1 (MQ=255) ccGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGgcgc > 2:341075/1‑149 (MQ=255) ccGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGgcgc < 1:169731/149‑1 (MQ=255) ccGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATCCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGgcgc > 1:178408/1‑149 (MQ=255) ccGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATCCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGgcgc > 1:241227/1‑149 (MQ=255) cGCAGGAAGGGCACTGGCGCGGCACCGGACGCTAGCTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCCTTCGGCAATCCAGCCACAGGCGTGGCACCGCAGTGCAGgcgcg > 2:230053/1‑148 (MQ=255) gCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCaa < 2:136970/149‑1 (MQ=255) aGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCAc > 2:274637/1‑149 (MQ=255) cgcgGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCGTGATGCAGCGTGAAGCTGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTaag > 2:270306/1‑149 (MQ=255) cACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTaagaaaga < 1:131626/149‑1 (MQ=255) cGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGaa < 1:96628/149‑1 (MQ=255) ggACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAAt < 1:319737/149‑1 (MQ=255) gtggCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTCGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCAACGCGGTTAAGAAAGAGAAGGACCTAGTTCTCAGCCTGTa > 1:106019/1‑149 (MQ=255) ggcgcAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGc < 1:45931/148‑1 (MQ=255) gcAGATGGGGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACCGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTgg < 1:179757/149‑1 (MQ=255) cAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGgc < 1:217263/149‑1 (MQ=255) tgctgcGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACg > 2:40595/1‑149 (MQ=255) gATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAAc < 2:20488/149‑1 (MQ=255) gATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAAc > 2:326110/1‑149 (MQ=255) tgtAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGGGcc > 2:285656/1‑149 (MQ=255) gtagtaGTGATCGCAACGTGGGCATCCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCa < 2:241227/149‑1 (MQ=255) gtagtaGTGATCGCAACGTGGGCATCCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCa < 2:178408/149‑1 (MQ=255) agtGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCAGANGAGNGNTTANCNCCGGCGCCAGGcct > 2:190678/1‑149 (MQ=255) gtGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCAGAcctg > 1:132948/1‑149 (MQ=255) aTCGCAACGTGGGCATTCGGCAAACCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCAGAcctgcct > 2:188008/1‑149 (MQ=255) cAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCAGACCTGCCTGCAc < 2:154808/149‑1 (MQ=255) tCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCAGACCTGCCTGCACCTTCTGACCttt < 2:145888/149‑1 (MQ=255) | GGTGCCCAGCCCCACGGGGACCAGGTGCGTGGAACCGCAGGAAGGGCACTGGCGCGGCACCGGACGCTGACTGTCACAGTGGTGGCAGCGCAGATGGTGCTGCGCCTGATGCAGCGTGTAGTAGTGATCGCAACGTGGGCATTCGGCAATCCAGCCACAGTCGTGGCACAGCAGTGCAGGCGCAAAGCCACGGCGGTTAAGAAAGAGAATGACCTGGTTATCAGCCTGTAAATGCTGGCGCATACGAGTGATTAACGCCGGAGCCAGACCTGCCTGCACCTTCTGACCTTT > CP009273/4115266‑4115556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |