| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,104,736 | C→T | 47.4% | intergenic (‑339/‑55) | opgC ← / → opgG | osmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,104,736 | 0 | C | T | 47.4% | ‑4.8 / 18.4 | 19 | intergenic (‑339/‑55) | opgC/opgG | osmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base C (2/8); new base T (5/4); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCA > CP009273/1104595‑1104877 | gAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATaa < 1:191875/149‑1 (MQ=255) tCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGcac < 1:341413/149‑1 (MQ=255) ggAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCtt > 2:127590/1‑149 (MQ=255) gAAAGTGAAGACGGTGGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACCCAAAGGGGGAAGTGCTTACTAATTATGa < 1:345619/149‑1 (MQ=255) gAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATa < 2:259747/149‑1 (MQ=255) gCAATCAGGTGCTTTATCCATGAGCCAGCGTGCTGAACGATACAGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGGTTACTAATTATGAAACATAAACTACAAat < 2:224588/149‑1 (MQ=255) aaTCAGGTGCTTAATCCATGAGCCTGCGTGACGACAGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTATAATTGATCGTATCGATATAAGCACACAAAGGGGGAAGGGCTTACTAATTATGAAACATAAACTACAAatga < 2:120668/149‑1 (MQ=255) gTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGaaaa < 2:288301/149‑1 (MQ=255) cTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATTGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAAGATGAGGTACAGATGATGaaaccgc > 2:280401/1‑145 (MQ=255) cAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGtgct < 1:330913/149‑1 (MQ=255) cGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCa < 1:127590/149‑1 (MQ=255) tGAACGATACCGGGATTCTGTTGGCGGAATGGCTGGTTATCCATTAAAGTAGATCGGGACGATATAAGCACACAAAGAGGAAAGTGCATACTAATTACAGAAGATAAACTACACACGATAACGAAGCGTTGGTGGATtggtgcgtgaag > 1:102447/1‑143 (MQ=255) ccGGGATTCGTGTGTCGCAATGGTTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACGACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCt < 2:52594/149‑1 (MQ=255) ccGGGAGTCTGTTGTCGGAATGGCTGGTTATCCATTAAAAGAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCt < 2:152042/149‑1 (MQ=255) cGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTg > 2:30333/1‑149 (MQ=255) gttgtCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTACTTATGAAACATAAACTACAAATGAGGAAAATGCGTTGGTTGCGTGCTGCAGTAATGTTAACCCTGTATACATCt > 2:138062/1‑149 (MQ=255) gtCGGAATGGCTGATTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAGACTACAAATGATGAAAATGCGTTGGTTGAGTTCTGCAGTAATGTTAACCCTGTATACATCTTCa > 2:244165/1‑149 (MQ=255) aTTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGATTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTATATACATCTTCAAGCTAGGCTTTCCGTATtga > 1:108435/1‑149 (MQ=255) tAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTc < 2:148681/149‑1 (MQ=255) atcggatcgATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCa > 1:300870/1‑149 (MQ=255) | GAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCA > CP009273/1104595‑1104877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |