Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,104,736 C→T intergenic (‑339/‑55) opgC ← / → opgG osmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,104,7360CT98.8% 280.4 / NA 85intergenic (‑339/‑55)opgC/opgGosmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (34/50);  minor base G (1/0);  total (35/50)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGC  >  CP009273/1104590‑1104881
                                                                                                                                                  |                                                                                                                                                  
tACACGAAGTCGATGGTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGa                                                                                                                                                  <  2:582816/149‑1 (MQ=255)
   acGAAGTCGATGCTTTTGGCTGTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCTTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGatat                                                                                                                                               <  1:138251/149‑1 (MQ=255)
   acGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGatat                                                                                                                                               <  1:1076411/149‑1 (MQ=255)
    cGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGAtata                                                                                                                                              <  2:591280/149‑1 (MQ=255)
      aaGGCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTTATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAg                                                                                                                                            <  2:998274/149‑1 (MQ=255)
           gATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGcacac                                                                                                                                       >  2:793888/1‑149 (MQ=255)
               cTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAg                                                                                                                                   <  1:221005/149‑1 (MQ=255)
                ttCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAgg                                                                                                                                  <  2:102672/149‑1 (MQ=255)
                     tCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGaa                                                                                                                             <  2:258558/149‑1 (MQ=255)
                       tttAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGt                                                                                                                           >  2:503663/1‑149 (MQ=255)
                        ttAGGAGAAGCACGGAAAGTGAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATGGATATAAGCACACAAAAGGGGGCGTGc                                                                                                                         >  2:640462/1‑149 (MQ=255)
                         tctgcgaAGCACCGAAAGTGAAAACGGTTGCAATCAGGCGCTTAATCCATGAGCCAGCGTGATGAACGATACCGGGCTTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGc                                                                                                                         <  1:529749/144‑1 (MQ=255)
                          aGGAGAAGCACGGAAAGTGAAAGCGGTTGCAATCAGGTGCTTAATCCAGGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCt                                                                                                                        <  2:962291/149‑1 (MQ=255)
                           ggAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCtt                                                                                                                       <  1:26368/149‑1 (MQ=255)
                            gagaAGCACGGAAAGTGAAAACGGTTGCAATCAGGAGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATCAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTa                                                                                                                      >  2:874640/1‑149 (MQ=255)
                                  cACGGAAAGTGAAAAGGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATGAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAAtt                                                                                                                >  2:916842/1‑149 (MQ=255)
                                  cACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAAtt                                                                                                                <  1:1317047/149‑1 (MQ=255)
                                   aCGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCGAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTa                                                                                                               >  2:7935/1‑149 (MQ=255)
                                   aCGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGcac                                                                                                                                         >  1:286802/1‑123 (MQ=255)
                                   aCGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGcac                                                                                                                                         <  2:286802/123‑1 (MQ=255)
                                   aCGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTa                                                                                                               <  2:64359/149‑1 (MQ=255)
                                    cGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTAt                                                                                                              <  2:260553/149‑1 (MQ=255)
                                     ggAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGGGCTTACTAATTATg                                                                                                             >  2:205799/1‑149 (MQ=255)
                                      gAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGa                                                                                                            <  2:458952/149‑1 (MQ=255)
                                       aaaGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGGGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGaa                                                                                                           <  2:1155569/149‑1 (MQ=255)
                                        aaGTGAAAACGGTTGCAATTTGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGaaa                                                                                                          <  2:367049/149‑1 (MQ=255)
                                        aaGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGaaa                                                                                                          <  2:609341/149‑1 (MQ=255)
                                        aaGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGaaa                                                                                                          <  2:847219/149‑1 (MQ=255)
                                              aaaCGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATaaa                                                                                                    <  1:851059/149‑1 (MQ=255)
                                                  ggTTGCAATCAGGTGCTTAATCCAGGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTAc                                                                                                <  2:231929/149‑1 (MQ=255)
                                                      gCAATCAGGTGCTTAATCCATGGGCCAGCGTGTTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAat                                                                                            <  2:1352351/149‑1 (MQ=255)
                                                      gCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAat                                                                                            <  2:1179237/149‑1 (MQ=255)
                                                        aaTCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGGGCTTACTAATTATGAAACATAAACAACAAatga                                                                                         >  2:847013/1‑149 (MQ=255)
                                                              gTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGaaaa                                                                                   >  1:467432/1‑149 (MQ=255)
                                                               tGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAAt                                                                                  >  1:123653/1‑149 (MQ=255)
                                                               tGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACGAATTATGAAACATAAACTACAAATGATGAAAAt                                                                                  <  2:674848/149‑1 (MQ=255)
                                                                 cTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGc                                                                                >  2:70850/1‑149 (MQ=255)
                                                                   tAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCgt                                                                              <  1:1163338/149‑1 (MQ=255)
                                                                      tCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTCTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTTCAAATGATGAAAATGCgttgg                                                                           <  1:1029282/149‑1 (MQ=255)
                                                                        cATGCGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAGTTATGAAACATAAACTACAAATGATGAAAATGCgttggtt                                                                         <  2:1058723/149‑1 (MQ=255)
                                                                        cATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTACTTATGAAACATAAACCACAAATAATGAAAAAGCgttggtt                                                                         >  2:798321/1‑149 (MQ=255)
                                                                         aTGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCgttggttg                                                                        <  1:72636/149‑1 (MQ=255)
                                                                         aTGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCgttggttg                                                                        >  1:1212992/1‑149 (MQ=255)
                                                                            aGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACGCAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGt                                                                     >  1:655670/1‑149 (MQ=255)
                                                                            aGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGt                                                                     <  1:813169/149‑1 (MQ=255)
                                                                                 gCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGaa                                                                                                                             >  2:72585/1‑89 (MQ=255)
                                                                                 gCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGaa                                                                                                                             <  1:72585/89‑1 (MQ=255)
                                                                                   ctGCTGAACGATACCGAGATTCTGTTGTCGTAAAGAGTGATTAACCATGAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGCAACATACACTACCAATGATGCAAACGCGTTGGTTGAGTGCTGCAg                                                              <  2:1387332/148‑1 (MQ=255)
                                                                                      cTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGa                                                                                                            >  2:532/1‑101 (MQ=255)
                                                                                      cTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGa                                                                                                            <  1:532/101‑1 (MQ=255)
                                                                                      cTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTaa                                                           <  2:123653/149‑1 (MQ=255)
                                                                                       tGACCGATACGGGGATTCTGGTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAAt                                                          <  1:51335/149‑1 (MQ=255)
                                                                                       tGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAAt                                                          <  2:1212992/149‑1 (MQ=255)
                                                                                              tACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAGGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTCAcc                                                   >  1:344680/1‑149 (MQ=255)
                                                                                               aCCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAAccc                                                  >  2:223250/1‑149 (MQ=255)
                                                                                                 cGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAatga                                                                                         >  1:653804/1‑108 (MQ=255)
                                                                                                 cGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAatga                                                                                         <  2:653804/108‑1 (MQ=255)
                                                                                                   ggATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGta                                              <  2:334228/149‑1 (MQ=255)
                                                                                                    gATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGtat                                             <  1:503663/149‑1 (MQ=255)
                                                                                                         tgttgtCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATAGGTCTTCTTGGTTTGTTGAGTGCTGCAGTAAGGTTAACCCTGTATACATc                                        >  1:477138/1‑149 (MQ=255)
                                                                                                         tgttgtCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAACGTTAACCCTGtatcaatc                                        >  1:688808/1‑144 (MQ=255)
                                                                                                          gttgtCGGAATGGCTGGTTATACATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGTTTACTAATTATGAAACATAAACTACCAATGATGCAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCt                                       <  1:1060932/149‑1 (MQ=255)
                                                                                                           ttgtCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCtt                                      >  1:199011/1‑149 (MQ=255)
                                                                                                             gtCGGAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGGTTACTGATTATGAAACATAAACTACAAATGATGAAAAAGCGTTGGTTGATTGCTGCAGAAATGTTAACCGTGTATACAGCTTCa                                    >  1:1088434/1‑149 (MQ=255)
                                                                                                                ggAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGc                                 <  2:1298078/149‑1 (MQ=255)
                                                                                                                 gAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCt                                >  2:749942/1‑149 (MQ=255)
                                                                                                                 gAATGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAAACCTGTATACATCTTCAAGCt                                >  2:734856/1‑149 (MQ=255)
                                                                                                                 gAATGGACGGATATCCATTAATATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCt                                <  1:1268542/149‑1 (MQ=255)
                                                                                                                 gAAGGGCTGGTAATCCAATAAAATAGATCGGATTGATAGAAGCACACAAAGGGGGAAGTGCTTACTAATTATTAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCt                                <  2:40815/149‑1 (MQ=255)
                                                                                                                  aaTGGCTGGTTATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTg                               >  2:1364232/1‑149 (MQ=255)
                                                                                                                   aTGGCTGCTTATCCAATAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAAAGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTgg                              <  1:847013/149‑1 (MQ=255)
                                                                                                                    tGGCTGGTTATCCATTAAAATAGAACGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTggg                             <  2:785477/149‑1 (MQ=255)
                                                                                                                           ttATCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCCAGCTGGGCTTTCAg                      >  1:177823/1‑149 (MQ=255)
                                                                                                                             aTCCATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAACGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTa                    <  1:208279/149‑1 (MQ=255)
                                                                                                                               ccATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTAtt                  <  1:749942/149‑1 (MQ=255)
                                                                                                                               ccATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTAtt                  >  2:354347/1‑149 (MQ=255)
                                                                                                                                cATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtg                 >  1:434089/1‑149 (MQ=255)
                                                                                                                                cATTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTAGACATCTTCAAGCTGGGCTTTCAGTATtg                 <  1:874640/149‑1 (MQ=255)
                                                                                                                                 aTTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtga                >  2:1068223/1‑149 (MQ=255)
                                                                                                                                 aTTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtga                >  1:1074146/1‑149 (MQ=255)
                                                                                                                                 aTTAAAATAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGCTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGACTtga                >  2:705032/1‑149 (MQ=255)
                                                                                                                                 aTTAAAACAGATCGGAGTGAACTACGCTCACAAAGGGGGAAGTGCTCAATAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCACTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtga                <  1:715903/149‑1 (MQ=255)
                                                                                                                                    aaaaTAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATtgatga             >  2:66144/1‑149 (MQ=255)
                                                                                                                                        tAGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTc         <  1:1316510/149‑1 (MQ=255)
                                                                                                                                         aGATCGGATTGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCg        >  2:1401854/1‑149 (MQ=255)
                                                                                                                                           atcggattgATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCa      >  1:1142501/1‑149 (MQ=255)
                                                                                                                                               gattgATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAaagc  >  1:790115/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TACACGAAGTCGATGCTTCTGTCTTTAGGAGAAGCACGGAAAGTGAAAACGGTTGCAATCAGGTGCTTAATCCATGAGCCAGCGTGCTGAACGATACCGGGATTCTGTTGTCGGAATGGCTGGTTATCCATTAAAATAGATCGGATCGATATAAGCACACAAAGGGGGAAGTGCTTACTAATTATGAAACATAAACTACAAATGATGAAAATGCGTTGGTTGAGTGCTGCAGTAATGTTAACCCTGTATACATCTTCAAGCTGGGCTTTCAGTATTGATGATGTCGCAAAGC  >  CP009273/1104590‑1104881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: