Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,111,161 C→A R293L (CGT→CTT)  lpxL ← lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,111,1610CA98.5% 213.6 / ‑5.8 67R293L (CGT→CTT) lpxLlauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (29/36);  minor base G (0/1);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTG  >  CP009273/1111016‑1111305
                                                                                                                                                  |                                                                                                                                                
ccGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGgt                                                                                                                                               <  1:202136/149‑1 (MQ=255)
    cTTTCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAATGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTaa                                                                                                                                           <  2:380321/149‑1 (MQ=255)
     ttCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAAc                                                                                                                                          <  2:332317/149‑1 (MQ=255)
       ccAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACca                                                                                                                                        >  1:1350254/1‑149 (MQ=255)
        cAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACcac                                                                                                                                       >  2:708114/1‑149 (MQ=255)
        aaGGGGGCGCAATCGAGAGAGCTCTGATAGCTCAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGCACGCATACAGGGCGGGTTTTAAAGCGAAGGTGTAACcac                                                                                                                                       >  2:305711/2‑149 (MQ=255)
         aGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACcaca                                                                                                                                      <  1:1187951/149‑1 (MQ=255)
           ggggCGCAATCCAGGGAGCTTTTATCGCTAAAGCAGGGGGCTTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACata                                                                                                                                    <  2:125844/149‑1 (MQ=255)
             ggCGCAATCCAGAGAGCTTTTACCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACAtata                                                                                                                                  <  1:436393/149‑1 (MQ=255)
                  aaTCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGct                                                                                                                             <  2:1145305/149‑1 (MQ=255)
                      cAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTgg                                                                                                                         >  1:1154899/1‑149 (MQ=255)
                           agCTTTTATCGCTAAATCAGGGGGATTCGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCACGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTACCCACATATACTGCTCTGGTGcag                                                                                                                    <  1:1221412/149‑3 (MQ=255)
                             cTTTTATCGCTAAACCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCcatc                                                                                                                  <  1:492974/149‑1 (MQ=255)
                              ttttATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCcatca                                                                                                                 >  1:2361/1‑149 (MQ=255)
                               tttATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGCAGTCCGTATCACATATACTGCTCTGGTGCcatcat                                                                                                                <  1:714173/149‑1 (MQ=255)
                                 tATCGCTAAATCAGGGGGATTTGCTGTGGTAAGGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCatga                                                                                                              <  1:687666/149‑1 (MQ=255)
                                         aaTCCGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCAtttt                                                                                                      <  1:1388543/149‑1 (MQ=255)
                                           tCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTc                                                                                                    <  1:1148909/149‑1 (MQ=255)
                                             aGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGa                                                                                                  <  1:879166/149‑1 (MQ=255)
                                              gggggATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGAc                                                                                                 >  2:8449/1‑149 (MQ=255)
                                              gggggATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGAATTAGTAGTTAGGTGTAACCACATATACTGCTCTGGTGCCATCATGAGGCATTTTTCGAc                                                                                                 >  1:698800/1‑149 (MQ=255)
                                               ggggATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATCTACTGCTCTGGTGCCATCATGATGCATTTTTCGAcc                                                                                                >  2:1327017/1‑149 (MQ=255)
                                                gggATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCa                                                                                               >  2:173471/1‑149 (MQ=255)
                                                  taTTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCAGTATGCATTTTTCGACCACt                                                                                             <  1:1066859/148‑1 (MQ=255)
                                                       gccgtgtcaaTGGCGGATGCCATTCTGAAGCAGCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGtt                                                                                        <  2:1332555/141‑1 (MQ=255)
                                                         tgtgGTAATGCCGGATGCCATTCTGAAGCAGCCGGCATGGGAGAGTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTGTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCa                                                                                      >  2:729883/1‑149 (MQ=255)
                                                          gtgGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCAt                                                                                     <  2:858045/149‑1 (MQ=255)
                                                          gtgGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGGTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCAt                                                                                     >  2:500829/1‑149 (MQ=255)
                                                          gtgGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGGGAAGGATCGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCAt                                                                                     >  2:5970/1‑149 (MQ=255)
                                                          ggggTAATGCCGGAGGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCAt                                                                                     <  1:586178/147‑1 (MQ=255)
                                                            ggTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATcc                                                                                   <  1:8449/149‑1 (MQ=255)
                                                               aaTGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACAACTTTGTTCATCCAcg                                                                                >  1:489687/1‑149 (MQ=255)
                                                                  gCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACgcgg                                                                             <  1:1443387/149‑1 (MQ=255)
                                                                  gCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTACGACCACTGTGTTCATCCACgcgg                                                                             <  2:97067/149‑1 (MQ=255)
                                                                    cGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACgcggcg                                                                           <  1:105617/149‑1 (MQ=255)
                                                                       aTGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTa                                                                        <  1:299131/149‑1 (MQ=255)
                                                                           cATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGttt                                                                    >  1:537636/1‑149 (MQ=255)
                                                                            aTTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTc                                                                   >  1:271295/1‑149 (MQ=255)
                                                                             ttCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAAc                                                                                                                                          >  1:558478/1‑77 (MQ=255)
                                                                             ttCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAAc                                                                                                                                          <  2:558478/77‑1 (MQ=255)
                                                                             ttCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCg                                                                  >  1:364700/1‑149 (MQ=255)
                                                                                  aaGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGAGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGcatc                                                             >  1:197282/1‑149 (MQ=255)
                                                                                      aTCCGGCATGGGAGATTTAATCGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTAAGCCCTCTTGGTTCATCCACGCGGCGGTAGTTTCGGCATCATcc                                                         <  1:710877/149‑1 (MQ=255)
                                                                                                ggAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGCCGTTTCGGCATAATCCAGTTGCGgag                                               >  2:367777/1‑149 (MQ=255)
                                                                                                ggAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATAAACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGgag                                               >  2:763539/1‑149 (MQ=255)
                                                                                                 gagaTTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATGCACGCGACGGTAGTTTCGGCATGATCCAGGGGCggcgc                                              >  2:57623/1‑146 (MQ=255)
                                                                                                  agaTTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGaa                                             <  1:1089412/149‑1 (MQ=255)
                                                                                                    aTTTAATAGCGTGAAGGAACGCCTGCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAaca                                           <  2:2361/149‑1 (MQ=255)
                                                                                                          tAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGCTTCGGCCATACCCCTGGGCGGAGAACACTCTgg                                     >  2:662233/1‑149 (MQ=255)
                                                                                                            gCGTGAAGGAACGCATTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCAGTAGTTTCGGCATCATCCAGTGGCGCAGAACAATCTggcg                                   >  2:1199648/1‑149 (MQ=255)
                                                                                                             cGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCCTCCACCCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTggcgg                                  >  1:195917/1‑149 (MQ=255)
                                                                                                               tGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGgca                                <  1:351685/149‑1 (MQ=255)
                                                                                                                      aCGCCTTACGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGGAGGTTCGGCATCATCCAGTGGCGGAGAAGACTCTGGCGGCCGCATAAt                         >  1:670189/1‑149 (MQ=255)
                                                                                                                           ttCCGGGCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAAtt                    <  1:708114/149‑1 (MQ=255)
                                                                                                                              cGGGCGTGTTTTAAAGCGAGGGTGTAACCACATATACTGCTCTGGTGCCACCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGAt                 <  1:137120/149‑1 (MQ=255)
                                                                                                                               gggCGTGTTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATGCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGACCACTCTGGCGGCAGCATACTCAGTTGATa                >  2:880856/1‑149 (MQ=255)
                                                                                                                                     gtTTTAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCttt          <  1:367777/149‑1 (MQ=255)
                                                                                                                                     gtTTTAAAGCGAAGGTGTAACCACATAAACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCttt          <  1:763539/149‑1 (MQ=255)
                                                                                                                                      ttttAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTTGTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTg         <  2:159550/149‑1 (MQ=255)
                                                                                                                                      ttttAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTg         >  1:446903/1‑149 (MQ=255)
                                                                                                                                      ttttAAAGCGAAGGTGTAACCACATATACTGCTCTGGTGACATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTg         <  2:747770/149‑1 (MQ=255)
                                                                                                                                            aaGCTAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATc    <  1:946553/149‑1 (MQ=255)
                                                                                                                                            aaGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTTCCATc    >  1:658837/1‑149 (MQ=255)
                                                                                                                                            aaGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATc    >  2:216564/1‑149 (MQ=255)
                                                                                                                                            aaGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATc    >  2:78936/1‑149 (MQ=255)
                                                                                                                                             aGCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCt   <  1:173471/149‑1 (MQ=255)
                                                                                                                                              gCGAAGGTGTAACCACATATACTGCTCTGGTGCCATCGTGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTg  <  2:1304145/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCGACTTCCAGGGGGCGCAATCCAGAGAGCTTTTATCGCTAAATCAGGGGGATTTGCTGTGGTAATGCCGGATGCCATTCTGAAGCATCCGGCATGGGAGATTTAATAGCGTGAAGGAACGCCTTCCGGGCGTGTTTTAAAGCGACGGTGTAACCACATATACTGCTCTGGTGCCATCATGATGCATTTTTCGACCACTTTGTTCATCCACGCGGCGGTAGTTTCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTG  >  CP009273/1111016‑1111305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: