Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,123,224 A→T intergenic (+39/‑71) yceM → / → murJ putative oxidoreductase/putative peptidoglycan lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,123,2240AT98.8% 272.1 / NA 82intergenic (+39/‑71)yceM/murJputative oxidoreductase/putative peptidoglycan lipid II flippase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (36/45);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCAC  >  CP009273/1123078‑1123364
                                                                                                                                                  |                                                                                                                                            
ttCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACGGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTgg                                                                                                                                            >  1:25100/1‑149 (MQ=255)
    ttGAATGTTCGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGa                                                                                                                                        <  2:644795/149‑1 (MQ=255)
    ttGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGGGAGTAAACCCTGTAACATCTGGCGGTGGCAAGTCACCGTAATCCTGCTAgc                                                                                                                                        >  1:120148/1‑148 (MQ=255)
      gAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACt                                                                                                                                      >  2:1004217/1‑149 (MQ=255)
         tgtgtgCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTAtt                                                                                                                                   >  2:136051/1‑149 (MQ=255)
          gtgtgCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACGAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCGTAATCCTGGTAGACCATTc                                                                                                                                  <  2:500713/149‑1 (MQ=255)
          gtgtgAAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATACACCGTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTc                                                                                                                                  <  1:647715/149‑1 (MQ=255)
          ggggggaaagCCTGACCGTTCCGGGAACCGCCGGCGAGCAGGCCGGGCTGGCGCAACGTATCGTTGACAAGGTCTGGCGGGATGCGGTGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATGCTCCATAAGCCTGGTAGACTATTc                                                                                                                                  <  2:405302/143‑1 (MQ=255)
             tgCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGcc                                                                                                                               >  1:845287/1‑149 (MQ=255)
             tgCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGGCGACTATTCGcc                                                                                                                               >  2:905420/1‑149 (MQ=255)
              gCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCct                                                                                                                              <  2:126182/149‑1 (MQ=255)
                aaaaCCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCctct                                                                                                                            <  1:1355932/149‑1 (MQ=255)
                       gACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGCAGCGGCTCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAgcg                                                                                                                     <  1:1059342/149‑1 (MQ=255)
                        aCAGTTCCGCAAGCCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAgcgc                                                                                                                    <  1:1140868/149‑1 (MQ=255)
                           gTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCgcctg                                                                                                                 <  1:71222/149‑1 (MQ=255)
                            ttCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCgcctgc                                                                                                                >  2:878415/1‑149 (MQ=255)
                            ttCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCgcctgc                                                                                                                >  2:1098092/1‑149 (MQ=255)
                            ttCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCATGGTAGCCTATTCTCCTCTTTCAGCgcctgc                                                                                                                >  2:1451857/1‑149 (MQ=255)
                             tCCGCAAACCGCCGGCCAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCgcctgcc                                                                                                               <  2:18412/149‑1 (MQ=255)
                               cGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCtt                                                                                                             <  1:216353/149‑1 (MQ=255)
                                 cAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGc                                                                                                           <  1:811676/149‑1 (MQ=255)
                                  aaaCCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCa                                                                                                          <  2:813636/149‑1 (MQ=255)
                                   aaCCGCCGGCGAACAGGCCGTGCTGGCTGAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGGAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAg                                                                                                         <  2:1173344/149‑1 (MQ=255)
                                   aaCCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAg                                                                                                         <  2:1224130/149‑1 (MQ=255)
                                         cgGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGttt                                                                                                   <  1:809037/149‑1 (MQ=255)
                                          ggCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGtttt                                                                                                  <  1:1342195/149‑1 (MQ=255)
                                            cGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCGGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGc                                                                                                <  1:526626/149‑1 (MQ=255)
                                              gaCAGGCGGGGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACAGCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCAAGGCAGGCgctgaaaga                                                                                              <  2:769919/148‑9 (MQ=255)
                                              aaCAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGccc                                                                                              >  1:967809/1‑149 (MQ=255)
                                                 aGGCCGGGCTGGCGCAACGGATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAACCCTGGGAGACCATCCACCCCTTTCGGCGCCTGCCTGGCAGGCGTTTTGCCCGTg                                                                                           <  2:316995/149‑1 (MQ=255)
                                                  ggCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTgg                                                                                          >  2:172308/1‑149 (MQ=255)
                                                  ggCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCCTgg                                                                                          <  2:180728/149‑1 (MQ=255)
                                                   gCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTggg                                                                                         >  1:70180/1‑149 (MQ=255)
                                                         cTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTCATCACACATTACTACGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGa                                                                                   >  1:633496/1‑149 (MQ=255)
                                                          tGGCGTAACGTATCGGTGACAAGCTCTGTCGCGATGCGATGATTGAATAAGCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGAt                                                                                  <  1:627724/149‑1 (MQ=255)
                                                          cttgtcaacgatacGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGAt                                                                                  >  1:1331435/14‑149 (MQ=255)
                                                            gcgcAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTa                                                                                <  2:25100/149‑1 (MQ=255)
                                                               cAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGaa                                                                             <  2:1270398/149‑1 (MQ=255)
                                                               cAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAACAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGaa                                                                             <  2:984498/149‑1 (MQ=255)
                                                                     aTCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATCGACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTGGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAAAACCGa                                                                       >  2:477322/1‑149 (MQ=255)
                                                                        gTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGa                                                                    >  2:1153183/1‑149 (MQ=255)
                                                                            aCAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAAttt                                                                >  2:456168/1‑149 (MQ=255)
                                                                            aCAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAAttt                                                                >  2:826520/1‑149 (MQ=255)
                                                                                gATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATttatta                                                            >  2:272590/1‑149 (MQ=255)
                                                                                gATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATttatta                                                            >  1:1382853/1‑149 (MQ=255)
                                                                                  cttGGCGCGATGCCGTGAGTGAAGGACCCCCGATACCTCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTCTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTaaa                                                          <  1:199106/147‑1 (MQ=255)
                                                                                    tGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTGGCAGGCGGTTTGCCCGTGGGTCTGGATTAGAACACCGAGGAATTTATTAAGAt                                                        >  2:1025362/1‑149 (MQ=255)
                                                                                      gcgcGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGa                                                                                                                                        >  1:150744/1‑67 (MQ=255)
                                                                                      gcgcGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGa                                                                                                                                        <  2:150744/67‑1 (MQ=255)
                                                                                        gcgatgcgatGAGTGAATAAACCCTGTAACATCCGGCGGTAGCAATTCACCATAATCCTGGTAGACTAGTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCt                                                    <  1:285646/149‑1 (MQ=255)
                                                                                          gattcgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTgg                                                  <  2:859550/149‑1 (MQ=255)
                                                                                          gatgcgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTCTTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGCACCCCGATGACTTTTCTAGAATCGCTgg                                                  >  2:1408414/1‑149 (MQ=255)
                                                                                               gatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGgccgcc                                             <  1:80262/149‑1 (MQ=255)
                                                                                               gatgAGTGAAGAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGCATTTATTAAAATCGCTGgccgcc                                             <  1:768940/149‑1 (MQ=255)
                                                                                                   aGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTCGACTTTTCGCCTCTTTCAGCGCCTGCCTTCCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAGTTAATTAAAATCGCTGGCCGCCGTCa                                         >  1:720775/1‑149 (MQ=255)
                                                                                                   aGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCa                                         <  1:136051/149‑1 (MQ=255)
                                                                                                     tGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGAt                                                                      >  1:159448/1‑118 (MQ=255)
                                                                                                     tGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGAt                                                                      <  2:159448/118‑1 (MQ=255)
                                                                                                       aaTAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGACCACCGATGAATGTATTAAAATCGCTGGCCGCCGTCAGATc                                     >  2:113519/1‑149 (MQ=255)
                                                                                                          aaaCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCACCCCGag                                  >  2:553965/1‑148 (MQ=255)
                                                                                                           aaCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTgg                                                  >  1:1345540/1‑132 (MQ=255)
                                                                                                           aaCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTgg                                                  <  2:1345540/132‑1 (MQ=255)
                                                                                                           aaCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATg                                 <  1:245513/149‑1 (MQ=255)
                                                                                                            aCCCTGTACCATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGa                                <  1:1016071/149‑1 (MQ=255)
                                                                                                            aCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGa                                <  2:845287/149‑1 (MQ=255)
                                                                                                              ccTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGAcc                              >  2:758587/1‑149 (MQ=255)
                                                                                                              ccTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGAcc                              >  1:290696/1‑149 (MQ=255)
                                                                                                              ccTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGAcc                              >  1:419306/1‑149 (MQ=255)
                                                                                                              ccTGTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATAGCTGGCCGCCGTCAGCCCGATGaac                              >  2:891155/1‑147 (MQ=255)
                                                                                                                tGTAACATCTGGTGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCAt                            <  1:747576/149‑1 (MQ=255)
                                                                                                                 gTAACATCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATg                           <  1:272590/149‑1 (MQ=255)
                                                                                                                      aTCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGACCGCCGGAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTc                      >  1:699701/1‑149 (MQ=255)
                                                                                                                       tCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTcg                     <  2:143604/149‑1 (MQ=255)
                                                                                                                       tCTGGCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTcg                     >  2:890894/1‑149 (MQ=255)
                                                                                                                        cTGGCGGTAGCAAGTCCCCCTAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTcgc                    <  2:714269/149‑1 (MQ=255)
                                                                                                                          ggCGGTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCgt                  <  1:1153183/149‑1 (MQ=255)
                                                                                                                          ggCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCgt                  <  2:1315374/149‑1 (MQ=255)
                                                                                                                              gTAGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGc              <  1:172308/149‑1 (MQ=255)
                                                                                                                                aGCAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAAGTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCtt            >  2:159537/1‑149 (MQ=255)
                                                                                                                                  cAATTCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGACTTGATTAGAAGCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTgg          <  1:1000505/149‑1 (MQ=255)
                                                                                                                                     ttCACCATAATCCTGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCtt       >  2:1079860/1‑149 (MQ=255)
                                                                                                                                          cATAATCCTGGTAGACTATTCGCCGCCTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCAc  <  2:1382853/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCAC  >  CP009273/1123078‑1123364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑