Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 1,130,039 G→A intergenic (+26/‑146) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,130,0390GA97.5% 249.0 / NA 81intergenic (+26/‑146)flgF/flgGflagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (45/33);  minor base C (1/1);  total (46/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGAC  >  CP009273/1129896‑1130179
                                                                                                                                               |                                                                                                                                            
cGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTCTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGGGTTAATTGAAAGGATACATGACAAGTATCAATTGcc                                                                                                                                         >  2:584376/1‑149 (MQ=255)
cGTTGCGGAAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAAGGCAGAAGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGcc                                                                                                                                         <  1:559234/149‑1 (MQ=255)
    gCGGCAATGAGCGACATGTTTGCCAGCGCGCGGCGTTTTAAAATGCACATGTAGTTTATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGAt                                                                                                                                     <  2:1087769/149‑1 (MQ=255)
     cGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATg                                                                                                                                    >  2:1268665/1‑149 (MQ=255)
           tGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAg                                                                                                                              >  2:984295/1‑149 (MQ=255)
               cGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTATCGATGAGTTAATTGCAAGGATACATCACAAGTATAAATTGCCCGATGCGCACGTTTa                                                                                                                          >  2:776672/1‑149 (MQ=255)
                gACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTAt                                                                                                                         >  2:1412508/1‑149 (MQ=255)
                   aTGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGACGGTGGTCAGCAGCGTCGATGATAACGCAGGGCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCgg                                                                                                                      <  2:1209314/149‑1 (MQ=255)
                    tggttGCCAGCGCGCGGCGTTTGGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCggg                                                                                                                     <  2:897796/146‑1 (MQ=255)
                     gATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGt                                                                                                                    >  2:283980/1‑149 (MQ=255)
                      aTTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGGTCTGTATTATAATACATGGCAAGTATAAATTGCCCGAGGCGCAAGTGTATCGGGTc                                                                                                                   >  1:40984/1‑149 (MQ=255)
                       ttGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTACCGGGTCt                                                                                                                  >  2:413163/1‑149 (MQ=255)
                         gCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTAt                                                                                                                >  2:124307/1‑149 (MQ=255)
                         gCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTCATCGGGTCtct                                                                                                                >  2:124294/1‑147 (MQ=255)
                           cAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATgg                                                                                                              <  2:326764/149‑1 (MQ=255)
                           cAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATAGCCCGACGCGCAAGTTTATCGGGGCTCTgg                                                                                                              >  2:88941/1‑149 (MQ=255)
                             gcgcgcGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATgggg                                                                                                            <  1:440382/149‑1 (MQ=255)
                              cgcgcgGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATggggg                                                                                                           <  1:274707/149‑1 (MQ=255)
                              cgcgcgGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATggggg                                                                                                           <  1:507512/149‑1 (MQ=255)
                                    gcgTTTTGAAATGTAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGTGCAAGTTTATCGGGTCTATGGGGGCAATCg                                                                                                     <  1:763758/149‑1 (MQ=255)
                                     cgTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGc                                                                                                    >  1:121737/1‑149 (MQ=255)
                                       ttttGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTACCGGGTCTATGGGGGCAATCACaa                                                                                                  >  2:1197169/1‑149 (MQ=255)
                                       ttttGAAATCCAGGTGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGCTAAGTTAATTGAACGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTCTATCAGGTCTAAGGGGGCAATCGCaa                                                                                                  >  2:538897/1‑149 (MQ=255)
                                        tttGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAAt                                                                                                 <  1:1025894/149‑1 (MQ=255)
                                        ttggAAATGCAGATGAAGGTGATCAGCAGCGGCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAAt                                                                                                 <  2:564162/146‑1 (MQ=255)
                                                 cAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGTCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAtt                                                                                        <  1:370087/149‑1 (MQ=255)
                                                   gATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAtttt                                                                                      >  1:461908/1‑149 (MQ=255)
                                                   gATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAtttt                                                                                      >  1:896937/1‑149 (MQ=255)
                                                    aTGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTg                                                                                     >  2:559285/1‑149 (MQ=255)
                                                                 gcagcGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAg                                                                        >  1:77695/1‑149 (MQ=255)
                                                                     cGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCg                                                                    <  1:567260/149‑1 (MQ=255)
                                                                        cGAGGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTCTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGAt                                                                 <  2:1068487/149‑1 (MQ=255)
                                                                         gatgatAACCCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTACAAATTGCCCAATGCCCAAGTTTACCGGGTCTACGGGGGCAAACGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATa                                                                <  2:1212927/149‑1 (MQ=255)
                                                                          atgatAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCACTTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                                               >  2:487707/1‑149 (MQ=255)
                                                                            gatAACGCAGGCCGTGCCAACCAATTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAgg                                                             >  1:1077777/1‑149 (MQ=255)
                                                                                aCGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGAAAAGGCggt                                                         >  2:774022/1‑147 (MQ=255)
                                                                                 cGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGTGAGCACTTGTAGGCCGGATAAGGCGttt                                                        >  2:1257630/1‑149 (MQ=255)
                                                                                  gCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTa                                                       >  1:1153737/1‑149 (MQ=255)
                                                                                      gCCGTGCCAAGCTACTGCTGTCGATGAGGTAATTGGAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGTCGGATAAGGCGTTTAcgcc                                                   <  2:778836/149‑1 (MQ=255)
                                                                                      gCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGGTTAcgcc                                                   >  1:1250023/1‑149 (MQ=255)
                                                                                        cGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                 >  1:207827/1‑149 (MQ=255)
                                                                                           gCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATGCCTTTCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                              >  1:286790/1‑149 (MQ=255)
                                                                                               accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                          <  1:514502/149‑1 (MQ=255)
                                                                                               accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                          >  1:1107184/1‑149 (MQ=255)
                                                                                               accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                          >  1:1438121/1‑149 (MQ=255)
                                                                                                 cacctgctgTCGATGAGTGAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGTGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                        <  1:1170796/146‑1 (MQ=255)
                                                                                                 caacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcct                                        <  1:584376/149‑3 (MQ=255)
                                                                                                        tgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGACCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGGACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACa                                 >  2:53050/1‑149 (MQ=255)
                                                                                                          tCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACata                               >  2:1344477/1‑149 (MQ=255)
                                                                                                          tCGATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTGGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACGAAGACata                               >  1:483809/1‑149 (MQ=255)
                                                                                                            gATGAGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATg                             >  1:10553/1‑149 (MQ=255)
                                                                                                               gAGTTAATTGAAAGGATACATGACAAGTAGAAAGTGCCCGATGGGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGGGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCAc                          <  2:40984/149‑1 (MQ=39)
                                                                                                                aGTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACt                         >  2:540878/1‑149 (MQ=255)
                                                                                                                 gTTAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACtt                        <  1:219507/149‑1 (MQ=255)
                                                                                                                  ttAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGGAAGAAGACATATGCACttt                       >  2:1124380/1‑149 (MQ=255)
                                                                                                                  ttAATTGAAAGGAGACATGCCAAGTATAACTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACttt                       <  1:1120024/149‑1 (MQ=255)
                                                                                                                   tAATTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTCACCGGTCGATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTg                      <  1:639521/149‑1 (MQ=38)
                                                                                                                     aTTGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTc                    >  2:1202183/1‑149 (MQ=255)
                                                                                                                      ttGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCGGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCa                   <  1:998659/149‑1 (MQ=255)
                                                                                                                      ttGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCa                   >  2:292033/1‑149 (MQ=255)
                                                                                                                      ttGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCa                   <  2:92482/149‑1 (MQ=255)
                                                                                                                      ttGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCa                   <  2:1107184/149‑1 (MQ=255)
                                                                                                                      atGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCa                   <  2:119461/148‑1 (MQ=255)
                                                                                                                       tGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCCCTTTGTccc                  >  2:693519/1‑147 (MQ=255)
                                                                                                                       tGAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCAc                  >  2:122175/1‑149 (MQ=255)
                                                                                                                        gAAAGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACt                 >  1:1299367/1‑149 (MQ=255)
                                                                                                                         aaaGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTa                                                       <  2:10923/110‑1 (MQ=255)
                                                                                                                         aaaGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTa                                                       >  1:10923/1‑110 (MQ=255)
                                                                                                                         aaaGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTa                >  1:657615/1‑149 (MQ=255)
                                                                                                                         aaaGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTa                >  2:585311/1‑149 (MQ=255)
                                                                                                                           aGGATACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCCATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAAt              >  1:571061/1‑149 (MQ=255)
                                                                                                                            ggatcaaTGACAAGTAGAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATc             <  1:710172/143‑1 (MQ=255)
                                                                                                                            ggATACATGACAAGTATAATTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATc             <  1:1030391/149‑1 (MQ=255)
                                                                                                                              aTACATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCa           <  2:461908/149‑1 (MQ=255)
                                                                                                                                aCATGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGAGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACt         >  2:642560/1‑149 (MQ=255)
                                                                                                                                 cATGGCAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTa        <  2:16830/149‑1 (MQ=255)
                                                                                                                                   tGACAAGTATAAATTGCCCGATGCGCAGGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACa      <  1:124294/149‑1 (MQ=255)
                                                                                                                                   tGACAAGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACa      <  1:124307/149‑1 (MQ=255)
                                                                                                                                       aaGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGtt                                                         <  1:378332/94‑1 (MQ=255)
                                                                                                                                       aaGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGtt                                                         >  2:378332/1‑94 (MQ=255)
                                                                                                                                       aaGTATAAATTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGAc  >  1:383389/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                            
CGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGAC  >  CP009273/1129896‑1130179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: