| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | CP009273 | 1972748 = | 0 (0.000) | 51 (0.940) | 35/294 | 0.6 | 100% | intergenic (‑294/‑486) | flhD/uspC | flagellar class II regulon transcriptional activator, with FlhC/universal stress protein |
| ? | CP009273 | = 2283592 | NA (NA) | noncoding (1/1195 nt) | IS5 | repeat region | |||||
ATGATTATTTACGGTGAGTTATTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < CP009273/1972895‑1972748‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGC < CP009273/2283592‑2283444 ATGATTATTTACGGTGAGTTATTTTGACTTTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAG < 2:405749/149‑1 TTATTTACGGTGAGTTATTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAG < 2:747738/149‑1 gtgACGGTGAGTTATTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTG < 1:23093/146‑1 cggttgttgATTTTGACTGTGCGCAAGATCGCTTTTCGATTATTCCTGTTTCATTTTTGATTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAA < 1:312288/140‑1 TGAGTTATTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAA > 2:108762/1‑149 TGAGTTATTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGA‑GAACAA > 1:260118/1‑149 AGTTATTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGT < 2:368931/149‑1 TTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTG > 2:382510/1‑149 ACTGTGGGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGGTAGCAAAGAGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATG < 2:429304/149‑1 CTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGA < 1:205664/149‑1 TGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGGGC‑GAACAAGACCCTGAT‑ATGAG > 2:715901/1‑149 gcgtGCATCATCCCATTTCTATTATTCCTGTTTTATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTAGGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGA < 1:196399/145‑1 CATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTT > 2:490347/1‑149 TCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑CTGAGATCATGTTTGT > 2:366318/1‑149 TCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGGAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGT > 2:640602/1‑149 CCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTC > 2:188458/1‑149 CGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGA < 2:454147/149‑1 ttgTCCTTTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTTAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCA < 1:297618/146‑1 ATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑CTTCGATCAGATTTGTCCTCTGGAGCCAT > 2:112369/1‑149 TCCTGTTTCATTCTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAG > 2:643791/1‑149 TTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATC > 2:713040/1‑149 ctcGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGG‑AGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGA < 2:252624/146‑1 CTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATa > 2:891758/1‑148 cTGCTAGCGTAGCGAATATCTTTTTAACAGATTGAAGTACACCCAAGGCAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGg > 1:82349/2‑148 GCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGT < 2:445873/149‑1 TAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCA > 1:471320/1‑149 GCGTAGCGAAAAACTTTTCAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATC > 1:770135/1‑149 AAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACC > 2:568192/1‑149 AAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTAACTGGTGATG‑TAGCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACC > 2:873948/1‑149 CTTTTTATCAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑CAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGC < 2:361805/149‑1 TAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACA < 2:773547/149‑1 ATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCA < 1:108762/149‑1 ACCCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAA > 1:357383/1‑149 CCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGC > 2:705097/1‑149 CCAAAACAAAAGTATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGC > 1:526629/1‑149 AAGTATGACTTATCCATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGA < 1:571114/149‑1 TATGACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCA > 2:319298/1‑149 GACTTATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGACATCAACTTACATTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAA > 1:523027/1‑149 TATACATTTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTGCGCCGACAGTGAATTCAGCAGTAAGCGCCGCCAGACCAGAcacga > 1:412752/1‑144 TTATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC < 2:471320/149‑1 ATGTTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT < 2:38992/149‑1 TTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTC < 1:865014/149‑1 TTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTC > 2:679585/1‑149 TTAAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTC < 1:530968/149‑1 TTAAGGAA‑GTGCGGAACAAGTCCCCGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTC < 1:467172/149‑1 AAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGCCAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAAATTTTCTTGTCCC > 1:506354/1‑149 AAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCC > 2:355495/1‑149 AAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCC > 1:165628/1‑149 AAGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCC > 1:784317/1‑149 AGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG > 2:52916/1‑149 AGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG > 1:759987/1‑149 AGGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCG > 1:882859/1‑149 GGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGC > 1:703196/1‑149 GGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGC > 2:134126/1‑149 GGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCACCGACAGTGAATTAAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCGTGTACCGC > 2:465070/1‑149 ATGATTATTTACGGTGAGTTATTTTGACTGTGCGCAACATCCCATTTCGATTATTCCTGTTTCATTTTTGCTTGCTAGCGTAGCGAAAAACTTTTTAACAGATTGAAATACACCCAAAACAAAAGTATGACTTATACATTTATGTTAAg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < CP009273/1972895‑1972748‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGC‑GAACAAGTCCCTGAT‑ATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGC < CP009273/2283592‑2283444 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |