Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,054,766 C→A G217C (GGT→TGT)  nac ← nitrogen assimilation regulon transcriptional regulator; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,054,7660CA98.0% 152.9 / NA 49G217C (GGT→TGT) nacnitrogen assimilation regulon transcriptional regulator; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (26/22);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAATCTTGAGTTC  >  CP009273/2054622‑2054906
                                                                                                                                                |                                                                                                                                            
agagaCTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATa                                                                                                                                          >  2:322974/1‑149 (MQ=255)
      tCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCCCAAATAACtttt                                                                                                                                    >  2:48347/1‑149 (MQ=255)
         tGGAAGGAGGGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGcc                                                                                                                                 <  2:889739/149‑1 (MQ=255)
             aGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGa                                                                                                                             <  2:732721/149‑1 (MQ=255)
                    gTAATGCGTGACATCCACCCATTTGCTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCg                                                                                                                      >  2:663042/1‑149 (MQ=255)
                    gTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGccgccg                                                                                                                      >  1:751250/1‑149 (MQ=255)
                           gTGACATCCACCCATTTACTGCGCCACATAACGAACGGGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTAAATTTCACAAATAACTTTTGCCGGGAGTCGCCGTAgggca                                                                                                               >  1:497855/1‑147 (MQ=255)
                                   cACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTAGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACATTTGCCGTGAGTCGCCGTAGGGAAAAAGCCtc                                                                                                       >  1:500110/1‑149 (MQ=255)
                                    aCCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCtca                                                                                                      >  2:692075/1‑149 (MQ=255)
                                     ccccTTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCtcat                                                                                                     <  2:38996/149‑1 (MQ=255)
                                             ctccgcCACACAACCAGCGCGCGGCCGTTTGGGCTAATACTGCACCGCCCAGTGCGCTGGCAATCCCGTCGGTAAGCGGGGCAATAGACTCAATGTCATAAATATCTTTTGCCGTGAGTCGCCGTAGGGAAAAATCCTCATCAACCCGa                                                                                             <  1:511853/146‑1 (MQ=255)
                                                cgcCACATAACGGGCGCGCGGCCGATGCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGt                                                                                          <  1:205064/149‑1 (MQ=255)
                                                cgcCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGt                                                                                          >  1:887726/1‑149 (MQ=255)
                                                   cacaTAACGAACGCGCGGCCGATTCGGGTAATACTGCACCGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCCCAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCCCATAAACCAGAAGTCtg                                                                                       >  1:39746/1‑148 (MQ=255)
                                                    acaTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGGAGGGAAAAAGCCTCATCAAAACGAAGTCTaa                                                                                      >  2:77412/1‑149 (MQ=255)
                                                      aTAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATa                                                                                    <  1:382570/149‑1 (MQ=255)
                                                      aTAACGAACGCGCGGCCGATTCGGGTAATACGGCAGCGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATCGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATa                                                                                    <  1:142111/149‑1 (MQ=255)
                                                           gaacgCGCGGCCGATTCGGGTAATACTGCAACGCCCATGACGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCGGTAGGGAAAAAGACTCCACCACACGACGTCTCATCGCACt                                                                               >  1:422392/1‑149 (MQ=255)
                                                                  cgGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCACCAACACAAAGTCTAATACCACTGTAATCa                                                                        >  1:199191/1‑149 (MQ=255)
                                                                  cgGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGAAAGCGTGGCAAGAGACGCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGGCTAATAGCACTGTAAtaa                                                                        >  1:524312/1‑147 (MQ=255)
                                                                   ggCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCCTCAACACGAAGTCTAATAGCACTGTAAGCAc                                                                       >  1:264981/1‑149 (MQ=255)
                                                                     ccGATTCGGGTAATACTGCAACGCCCATGCCGCGGGCAATCGCTGCGGTAAGCGAGGCAAAAGAATCAATTTCACAAATAACTTTTGCCGTGAGAAAAAAAAAAAAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTg                                                                     <  1:581798/149‑1 (MQ=255)
                                                                      cGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTgg                                                                    >  2:811893/1‑149 (MQ=255)
                                                                      cGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTgg                                                                    >  2:226571/1‑149 (MQ=255)
                                                                      cGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTgg                                                                    <  2:603997/149‑1 (MQ=255)
                                                                      cGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTgg                                                                    <  2:539857/149‑1 (MQ=255)
                                                                       gATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCTTGGCAATAGACTCAATTTCACAAATGACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTggg                                                                   <  2:642331/149‑1 (MQ=255)
                                                                        aTTCGGGCAACACTGCAACGCCCATGCCGCTGGCAATCGCTTCGTTAAGCGTGGCAATAGACGCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTgggg                                                                  <  1:252437/149‑1 (MQ=255)
                                                                         ttCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAACAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGga                                                                 <  1:531862/149‑1 (MQ=255)
                                                                            gggTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGaa                                                              <  1:195861/149‑1 (MQ=255)
                                                                                  tACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGt                                                        <  2:706229/149‑1 (MQ=255)
                                                                                   aCTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAgggt                                                       >  2:381177/1‑147 (MQ=255)
                                                                                     tGCAACGCCCATGCCGCTCGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCa                                                     >  2:655961/1‑149 (MQ=255)
                                                                                       cAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCAtt                                                   >  2:834906/1‑149 (MQ=255)
                                                                                              cATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCaa                                            <  2:751250/149‑1 (MQ=255)
                                                                                              cATGCCGCTGGCAATCGCTGCGGTAAGAGTTGAAATAGTTGCATTTACACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCaa                                            <  2:605540/149‑1 (MQ=255)
                                                                                                   cGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTAATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGGTCATTTGCGCAATAGCa                                       >  2:231057/1‑149 (MQ=255)
                                                                                                     cTGGCAATCGCTGCGGTAAGCGTGGCCAAAGACTCACTTTCCCAAATAACGTATGCAGTCAGTTCCCGACGGGAAACAGACTCATCAACACGGAGGCTAGTAGGACTGTAATCACTGGGGAGAACGAGAATCAATAGCGCacgaagaat                                     >  1:269760/1‑141 (MQ=255)
                                                                                                     atcgcagtgtgtGCGGTAAGCGTGGCAATAGACTCAAGTTCACAAATAACTTGTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCAtt                                     <  1:757138/138‑1 (MQ=255)
                                                                                                       ggCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTca                                   <  2:776854/149‑1 (MQ=255)
                                                                                                          aaTCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGGGAAAGAGGGTCATTTGCGCAATAGCATTCCCAt                                >  1:711014/1‑149 (MQ=255)
                                                                                                           aTCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGGTCATTTGCGCAATAGCATTCACATc                               >  2:351077/1‑149 (MQ=255)
                                                                                                                tGCGGTAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGc                          >  2:534067/1‑149 (MQ=255)
                                                                                                                     tAAGCGTGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGc                     <  1:207147/149‑1 (MQ=255)
                                                                                                                           tGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCggg               <  1:663042/149‑1 (MQ=255)
                                                                                                                           tGGCAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGGAATCACTGGGGAGAAAGAGGTTCATTTGCGCACTAGCATTCACATCAACGCATTGCCCCggg               >  1:567690/1‑149 (MQ=255)
                                                                                                                              cAATAGACTCAATTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACCTCAACGCCTTGCCCCGGGCaa            >  1:682874/1‑149 (MQ=255)
                                                                                                                                    aCTCAATTTCACAACTAACTTTTGCGGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAATCTTGa      <  2:547556/149‑1 (MQ=255)
                                                                                                                                        aaTTTCACAAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAATCTTGAGTTc  >  1:365571/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                            
AGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCGGCCGATTCGGGTAATACTGCAACGCCCATGCCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTTGCCCCGGGCAATCTTGAGTTC  >  CP009273/2054622‑2054906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑