Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,519,268 Δ1 bp 3.8% intergenic (+18/+21) yfeN → / ← yfeR putative outer membrane protein/transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,519,2680T. 3.8% 296.8 / 6.8 104intergenic (+18/+21)yfeN/yfeRputative outer membrane protein/transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base T (57/38);  new base . (2/2);  total (61/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-01

Only 100 of 106 total aligned reads displayed.

GCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT  >  CP009273/2519122‑2519416
                                                                                                                                                   |                                                                                                                                                    
gcAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTACCGATATGGGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACATCTGATTGATTACTGCCGGGGCTcc                                                                                                                                                    >  1:796532/1‑149 (MQ=255)
    ttGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCt                                                                                                                                                      >  2:980109/1‑143 (MQ=255)
     tGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCa                                                                                                                                               <  1:1226941/149‑1 (MQ=255)
      gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAg                                                                                                                                              <  1:1100593/149‑1 (MQ=255)
       cACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGCATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTccc                                                                                                                                                   >  2:461974/1‑143 (MQ=255)
         cTGTGGTGGAAGGCAACATCACACCTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACGCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                           <  1:1203494/149‑1 (MQ=255)
         atttagtagaagacAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                           <  1:1087885/136‑1 (MQ=255)
          tgtgGTGGAATGCAACATCACACATTACTGCGGGGATTCAGTATCGATATGCGAATGACAAGCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGACTCCCGGCAGTATg                                                                                                                                          >  1:795724/1‑149 (MQ=255)
            tggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGaa                                                                                                                                        >  1:812477/1‑149 (MQ=255)
                 gAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTCCTGCCGGGGCTCCCGCCAGTCTGa                                                                                                                                         >  2:822718/1‑143 (MQ=255)
                   aTGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCActct                                                                                                                                >  1:1259892/1‑149 (MQ=255)
                    tGCAACATCACTCATGTCTACGGGGATTCAGTATCGGTATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatcaat                                                                                                                                      <  2:308412/143‑7 (MQ=255)
                    cgaaaCATCACACATTACTACGGGGGTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAAGATGCAtt                                                                                                                                      >  2:1053518/4‑143 (MQ=255)
                      cAACATCACACATTCCTACGGGGATACAGTATCTATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAAGCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCTGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                              <  1:793047/149‑1 (MQ=255)
                      cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCTATGCAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                              >  1:623443/1‑149 (MQ=255)
                              cacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                                      <  1:481393/149‑1 (MQ=255)
                                  ttACTACGGGTATTCAGTATCGATATGCTGATTACTATCTGGGTGAAGATTTTTACCAGGATGCGATAATCTACCCCATCAAATTTAACTTCTGATTGATTACTGGCGGGGCTCCCGGCAGTATTAATTCACTCTATATCTGATACAAc                                                                                                                  <  1:376034/149‑1 (MQ=255)
                                  ttACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGa                                                                                                                        <  2:793293/143‑1 (MQ=255)
                                  ttACTACGGGGATTCAGTATCGATATGCGGAGGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTgc                                                                                                                        >  2:792069/1‑142 (MQ=255)
                                     cttcGGGCATTCAGTATCGATATCCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATTATTTCACTCTATATCTGATACAACGGa                                                                                                               <  1:446035/146‑1 (MQ=255)
                                     ctacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGa                                                                                                               <  1:428123/149‑1 (MQ=255)
                                       acGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCCGTGCGGATTCACCCTATATCCGTGACAACGGacg                                                                                                             >  1:109099/1‑147 (MQ=255)
                                        cGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcc                                                                                                            >  1:1083937/1‑149 (MQ=255)
                                              ttCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCCCTCTATATCTGATACAACGGATCCCCTTcc                                                                                                      >  1:5611/1‑149 (MQ=255)
                                              ttCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                      >  1:478713/1‑149 (MQ=255)
                                               tCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATccc                                                                                                           <  2:850554/143‑1 (MQ=255)
                                                cggTATCGAGATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcccc                                                                                                          <  2:623587/141‑1 (MQ=255)
                                                cAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCg                                                                                                    >  1:419061/1‑149 (MQ=255)
                                                 aGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGc                                                                                                   <  1:599655/149‑1 (MQ=255)
                                                    aTCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGACAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                >  1:800342/1‑149 (MQ=255)
                                                     tCGATATGCGGATGACAAGCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTACATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTACATCTGATACAACGGATCCCCTccca                                                                                                     >  2:727903/1‑142 (MQ=255)
                                                      cGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGc                                                                                              >  1:1221617/1‑149 (MQ=255)
                                                        atatGCCGAAGACTAACCTGGCGAAAATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGTGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                  <  2:734492/143‑1 (MQ=255)
                                                         tatCCGGATGACAAACTGGGCGAAGATTTTTAGCAGGATGCGATCATCTACTCCATCAAATTTAACTTCTGATCGATTACTGCCGGGGCGCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCCTCCCGccc                                                                                                 >  2:814014/1‑143 (MQ=255)
                                                          atGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                          >  1:1239437/1‑149 (MQ=255)
                                                          aggCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                <  2:941191/141‑1 (MQ=255)
                                                             cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgc                                                                                       >  1:1157979/1‑149 (MQ=255)
                                                                aTGAAAAGCGGGGCGAAGAATATTACCAGGATGCGATAAAATACACCACCTAAGTTAACTGCCGATTGGTCGCTTCCGGTGCCAAACGCAGTATTAAGGCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                    <  1:953373/149‑1 (MQ=255)
                                                                  gACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCCTTAGCGCAtt                                                                                  >  1:575052/1‑149 (MQ=255)
                                                                           ggCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                               >  2:431181/1‑143 (MQ=255)
                                                                             cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                       <  1:640903/149‑1 (MQ=255)
                                                                              gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTCGCGCATTGCCCTg                                                                            >  2:901928/1‑143 (MQ=255)
                                                                              gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                      >  1:1235433/1‑149 (MQ=255)
                                                                                aGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTa                                                                    >  1:710562/1‑149 (MQ=255)
                                                                                   tttttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                                 <  1:286236/149‑1 (MQ=255)
                                                                                     tttccccgaaTGCGATAATCTACTCCATCAAATTTCACTTCTGCTTGATTACTGCCGGGGCTCCCGGCAGTATGCATTCACTCTATATCGGATACAACGGCTCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt                                                                     <  2:1127638/134‑1 (MQ=255)
                                                                                     tttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCGCCCGGCAGTATGCATTCTCTCTATATCTGATAACACGGATCCCCTTCCCGCCCAGCCATCAGCGCATTGCCCTGGtcgctg                                                                     >  2:271541/1‑139 (MQ=255)
                                                                                            tgttGCGATAATCTACTCCATCAAACTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATa                                                        <  1:301/146‑1 (MQ=255)
                                                                                             gATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaa                                                       <  1:605800/149‑1 (MQ=255)
                                                                                              aTGCGATAACCTACTCCATCAAATTTACCTCCGGATTGACTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                      <  1:120186/149‑1 (MQ=255)
                                                                                               tGCGATACTCTACGCCATCAAATGTAACTTCTGATTGGTTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTccc                                                           <  2:397975/143‑1 (MQ=255)
                                                                                               tGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATACCTGATACAACGGATCCCCTCCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTccc                                                           <  2:397815/143‑1 (MQ=255)
                                                                                                 cGACAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCCTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCAt                                                         <  2:904384/143‑1 (MQ=255)
                                                                                                         tACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATCAAGCTTc                                                 >  2:390267/1‑143 (MQ=255)
                                                                                                         tACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAACGCTTc                                                 >  2:1031422/1‑143 (MQ=255)
                                                                                                            tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                        >  1:1023685/1‑149 (MQ=255)
                                                                                                            atcATAAAATTTAACTTCTGTTTGCGTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                        <  1:939866/147‑1 (MQ=255)
                                                                                                              taTCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                      <  1:320138/148‑1 (MQ=255)
                                                                                                              cATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                      >  1:831453/1‑149 (MQ=255)
                                                                                                               aTCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGATTCAGCGGCGGTGGa                                     >  1:572701/1‑149 (MQ=255)
                                                                                                                tCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaa                                    >  1:1195578/1‑149 (MQ=255)
                                                                                                                tCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaa                                    >  1:10064/1‑149 (MQ=255)
                                                                                                                 cAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTCTATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGGGGGGGaaa                                   >  1:339624/1‑149 (MQ=255)
                                                                                                                    ctttAACTTCTGATTGATTACTGCCGGGCCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                      <  2:790586/142‑1 (MQ=255)
                                                                                                                       tAACTTCTGATTGATTACTGCCGGGGTTCCCGGCAGTGTGAATACCCTCTATATCTGTTTCATCGGCTCCCCTTCCCTCCCAGCCATGAGCGCATTGCCCTGCTCTCGTACCACGTCCCATATAGCTTCAGCGGCGGtcgtaa                                   <  2:94372/143‑6 (MQ=255)
                                                                                                                       tAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaaa                                   <  2:703260/143‑1 (MQ=255)
                                                                                                                         attccTGATTCATTACTGCCGGGGCTCCCGGCAGTACGAACGTATGCTATATCGGATACACCGTATGGCCGGCCCGCCCAGCTGTTGTTTCATTGCCTTTGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                           <  1:953162/145‑1 (MQ=255)
                                                                                                                         aCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                           <  1:408154/149‑1 (MQ=255)
                                                                                                                          cTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                          >  1:768278/1‑149 (MQ=255)
                                                                                                                          cTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCACTGAAGCTTCAGCGGCGGGGGAAAGTg                                >  2:996416/1‑143 (MQ=255)
                                                                                                                           ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGATTCAGCGGCGGGGGAAAGTGAACGGtt                         >  1:560186/1‑149 (MQ=255)
                                                                                                                           ctCTGATTGAATGCTGCCGGGGCTCCCGGGAGTATGAATTCGCTCTATATCTGATACAACGGAGCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTTGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                         <  1:495474/148‑1 (MQ=255)
                                                                                                                             cTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCGGCCATCAGCGCATTGCCCTGGTCTCTTCCCACGTCCCATAAAGCTGCAGCCGCGGTGGACAGTGAAc                             >  2:737047/1‑143 (MQ=255)
                                                                                                                              tgattgatTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAGGTGAAGGGttctt                      >  1:666734/1‑149 (MQ=255)
                                                                                                                               gcttgattACTGCCGGGGCTCCCGGCAGTATGAATTCCCTCTATATCTGATACAACGGAGCCCCTTCCCGCCCAGCCATTCGCGCATTGCCCTGGTCTCGTACTACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                           <  2:688612/141‑1 (MQ=255)
                                                                                                                               gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                           >  2:22189/1‑143 (MQ=255)
                                                                                                                               gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                     <  1:537917/149‑1 (MQ=255)
                                                                                                                               gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGACGAAAGTGAAggg                           >  2:795574/1‑143 (MQ=255)
                                                                                                                               gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCGTCCCGCGCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                           >  2:344871/1‑143 (MQ=255)
                                                                                                                                attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAGGCTTCAGCGGCGGTGGGAAGTGAACGTTTCTTcc                    >  1:3130/1‑149 (MQ=255)
                                                                                                                                attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                          >  2:557311/1‑143 (MQ=255)
                                                                                                                                attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTcc                    >  1:654468/1‑149 (MQ=255)
                                                                                                                                 ttgattACTGCCGGGGCCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAAGCCAAATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                   <  1:634236/149‑1 (MQ=255)
                                                                                                                                    attACTGCCGGGGCTCCCGGCAGTATGTATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGTATTGCCCTGGTCTCGTACCACGTCCCATAAAGCGTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg                <  1:368131/149‑1 (MQ=255)
                                                                                                                                    attACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGGTCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGAGGTGGAAAGTGAACGGTTCTTCCTgcg                >  1:739534/1‑149 (MQ=255)
                                                                                                                                    attACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt                      >  2:1093783/1‑143 (MQ=255)
                                                                                                                                     ttACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc               >  1:788145/1‑149 (MQ=255)
                                                                                                                                     atACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc               >  1:322513/2‑149 (MQ=255)
                                                                                                                                       accgCCGGGGCCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                   <  2:453333/140‑1 (MQ=255)
                                                                                                                                       aCTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGGTCTTCCt                   >  2:879169/1‑143 (MQ=255)
                                                                                                                                       aCTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                   <  2:361868/143‑1 (MQ=255)
                                                                                                                                         tGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa           >  1:165433/1‑149 (MQ=255)
                                                                                                                                          gCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAg          <  1:638797/149‑1 (MQ=255)
                                                                                                                                           ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAGCGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACAGCCCATAAAGCGTCGGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAcaggc         >  1:123011/1‑145 (MQ=255)
                                                                                                                                           ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTGGCGCATTGCCCTGGTCTCGTACCACGTCCCTTCAAGCTTCACCCGCGGTGGAAAGGGACGGGTTCTTCCTgcgc               >  2:964533/1‑143 (MQ=255)
                                                                                                                                           ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGc         >  1:1109928/1‑149 (MQ=255)
                                                                                                                                                 gCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCCTAAAGCTTCAGCGGCGGAGGAAAGTGAACGGTGCGTCGTGCGCACCAg          >  2:36935/1‑143 (MQ=255)
                                                                                                                                                 gCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGCCTCGTACCACGTCCCATCAAGCTTCAGCGGCCGTGGAAACTGAACGCTTCTCCGTGCGcccccg          >  2:987160/1‑141 (MQ=255)
                                                                                                                                                  cTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTCCTTCCTCCGCCCCAGc         >  2:376707/1‑143 (MQ=255)
                                                                                                                                                   tCCCGGCATTATGAATTCCCTCTATATTTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt  <  1:466798/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT  >  CP009273/2519122‑2519416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: