| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 753,013 | G→T | 56.0% | E551* (GAA→TAA) | sdhA → | succinate dehydrogenase, flavoprotein subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 753,013 | 0 | G | T | 56.0% | 21.9 / 67.5 | 59 | E551* (GAA→TAA) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base G (14/12); new base T (19/14); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTC > CP009273/752869‑753161 | tCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATCGCCGCTTCGACTTCCCGGATCGCGATGATGaaaa > 1:1000681/1‑149 (MQ=255) gAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCGACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCAGACCGTGATACTGAAAACTGGCt > 1:26184/1‑149 (MQ=255) ttCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGAACGTGAGGATTAAAACTg > 2:710739/1‑143 (MQ=255) cAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGGAGGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGc > 2:1046561/1‑143 (MQ=255) cacCCAGCGCGTTGGGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCtgt < 2:759566/143‑1 (MQ=255) cccAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGc < 2:1010809/143‑1 (MQ=255) cggcgcGTCGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCt < 1:335441/147‑1 (MQ=255) cAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGcacagccagtttta < 1:165059/149‑14 (MQ=255) cgcgTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACGCCCTGTa > 1:663532/1‑149 (MQ=255) aGTGCCTGGAACTGGAGAACCTGATGGTAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCTACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCa < 1:739377/149‑1 (MQ=255) gTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTAGAATATTCGTATCGTGATGCTGACAACTGGCTGTGCCACTCCCTGTATCTGCCag > 1:619605/1‑149 (MQ=255) ccTGGAACTGGATAACCTGATGGAACCGGCGTATGCAACGGCTGTTGCTGCCAACTTCCGTACCGAAAGCCGTGGTGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGc < 2:390355/143‑1 (MQ=255) cTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCCCTGCCTGTATCTGCCAGAGTCGGAATc > 1:939308/1‑149 (MQ=255) gATACCCAGATGGAAACGGCGTATGCAACCGCTGTTTCGGCCAACTTCCGTACCGAAAGCCGTGGCGCGCCTAGCCGCTTCGACTTCCCGGATGGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGa < 2:406354/143‑1 (MQ=255) tAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGa > 1:139918/1‑149 (MQ=255) aaCCTGACGGAAACGGCGGATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATc < 2:535657/143‑1 (MQ=255) ccTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGCCGGAATCCATGAcac > 1:1062990/1‑147 (MQ=255) cTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTGTCTGCCAGAGGCGGAATCCAt > 2:771628/1‑143 (MQ=255) tGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCCTAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACgcgc > 1:260828/1‑149 (MQ=255) aTGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGCTTAAAACTGGCTGGGCCACTCCCTGTATCTGCCAGAGTCGGAACCCATGACGCGCCg > 1:1110931/1‑149 (MQ=255) aTGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCACATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGAGCCg > 1:1109867/1‑149 (MQ=255) aTGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCACATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAAAGTCGGAATCCATGACGCGCCg > 1:990328/1‑149 (MQ=255) ggtaaCGGCGTATGCAACGGCTGTTTCCGCCACCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGAcgc < 2:280381/140‑1 (MQ=255) ggAAACGGCGTATGCAACGGCTGTATCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGCCGGAATCCAAGAcgc > 2:281996/1‑143 (MQ=255) gAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTCGCGCGCATAGCCGCTTCGACTTCCCGGATGGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGACAGAGTCGGAATCCATGAcgcg > 2:188175/1‑143 (MQ=255) gCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCaa < 1:1252229/149‑1 (MQ=255) gCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCACGACGCGCCGAAg < 2:903489/143‑1 (MQ=255) cGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAAc < 1:1031410/149‑1 (MQ=255) tATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACAt < 1:1056951/149‑1 (MQ=255) tATGCAACGGCTGTTTCCGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGt < 2:756735/143‑1 (MQ=255) gaTGCAACGTCTGTTCCGGCCACCTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTTGACTTCCCGGATCGTGACTATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGt < 2:850726/142‑1 (MQ=255) tGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCACCATGGAAc > 2:872953/1‑143 (MQ=255) gTTTCTGCCAACTTCCGTACCGACAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGGGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAAcc < 2:962755/143‑1 (MQ=255) gTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAAcc < 2:337513/143‑1 (MQ=255) tttCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCAGCTTCGACTTCACGGATCGTGATGATGAAAACTGGCTGTGCCACTGCCTGTATCTGCCAGAGTCGGAATCCATGAGGCGCCGAAGCGCACACATGGGACCGAAACTg > 1:316845/1‑149 (MQ=255) cTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGCTCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCCACATGGAACCGAAGCTgcgc > 1:386167/1‑149 (MQ=255) cTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCGGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaa > 2:900259/1‑143 (MQ=255) cTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaa < 2:766412/143‑1 (MQ=255) tGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc > 1:513288/1‑149 (MQ=255) attCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc > 2:698196/2‑143 (MQ=255) gTACCGAGAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCccg < 1:161577/149‑1 (MQ=255) gTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCCCTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCccg > 1:1149890/1‑149 (MQ=255) tACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCccgc > 1:557868/1‑149 (MQ=255) tACCGAAAGCCGTGACGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGGCAACATGAAAACGAAACTGCGCCCGGCGTTCccgc > 1:353515/1‑149 (MQ=255) ccGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCCCTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGa > 2:456303/1‑143 (MQ=255) cGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCg < 1:1185690/149‑1 (MQ=255) tGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATCACGCGCCCAAGCGTCAACATGGACCCGAAACTGCGCCCGGCCTTCCCGCCGAAGa > 2:954687/1‑143 (MQ=255) cgcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactt > 1:1227440/1‑149 (MQ=255) gcgcATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGtactta < 1:1297433/149‑1 (MQ=255) aTAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTaa < 1:1098446/149‑1 (MQ=255) gCTTCGACTTCCCGGATCGTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGga > 1:693272/1‑149 (MQ=255) gCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATTTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACCTTGCACCGAAACTGCGCCCGGCAATCCCGCCGCAGACTCATACTTACTaa > 2:618380/1‑143 (MQ=255) ttCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTCATGCGgaga > 1:1215335/1‑149 (MQ=255) cccGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACa < 2:21754/143‑1 (MQ=255) ccGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAAt > 1:71913/1‑149 (MQ=255) aTCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATgaga < 1:995781/149‑1 (MQ=255) gTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATg < 2:441124/143‑1 (MQ=255) gTGATGATTAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTc < 1:662299/149‑1 (MQ=255) gTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAGCATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTAGTAATGCGGAGACAGGAAAATg > 2:814752/1‑143 (MQ=255) gaAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTc > 1:1289438/1‑149 (MQ=255) | TCCAGCGAGTTCAACACCCAGCGCGTTGAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTC > CP009273/752869‑753161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |