Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,419,720 T→C 11.2% D183G (GAT→GGT)  hisJ ← histidine/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,419,7200TC11.2% 261.9 / 21.5 108D183G (GAT→GGT) hisJhistidine/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (48/46);  new base C (4/8);  total (53/55)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

Only 100 of 108 total aligned reads displayed.

TCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGT  >  CP009273/2419579‑2419866
                                                                                                                                             |                                                                                                                                                   
tCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATc                                                                                                                                                    <  2:963273/143‑1 (MQ=255)
tCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGaa                                                                                                                                              >  1:1299955/1‑149 (MQ=255)
   ggcAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAAcgc                                                                                                                                           <  1:104557/148‑1 (MQ=255)
   cgcAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTg                                                                                                                                                 >  2:925001/1‑143 (MQ=255)
   cgcAGTTCGTTATCTTCTTTACGCAGGCCAATGCCAGTACCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGACTTTGTAATCTTTGCCGACAGGTTGTTTGAGGAAACCTTCGCTGACGGCGACCTCATCCTg                                                                                                                                                 >  2:517350/1‑143 (MQ=255)
    gcAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAAcgcg                                                                                                                                          <  1:830112/149‑1 (MQ=255)
    gcAGTTCGTTATCTCCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAAcgcg                                                                                                                                          <  1:172253/149‑1 (MQ=255)
     cAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCgg                                                                                                                                         <  1:444985/149‑1 (MQ=255)
     cAGTTCGTTACCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCgg                                                                                                                                         <  1:489684/149‑1 (MQ=255)
      aGTTCGTTATCTTCTTTACGCAGGCACATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGc                                                                                                                                        <  1:1002014/149‑1 (MQ=255)
          cGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAAcgcg                                                                                                                                          <  2:903412/143‑1 (MQ=255)
           gTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCgg                                                                                                                                         <  2:1128866/143‑1 (MQ=255)
           gTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCaa                                                                                                                                   >  1:1201831/1‑149 (MQ=255)
           gTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACCGGCAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCaa                                                                                                                                   >  1:1285736/1‑149 (MQ=255)
            ttATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGCCCTCATCCTGGAACGCGGCCTCAAt                                                                                                                                  >  1:68645/1‑149 (MQ=255)
             tATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACCGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTGGCTGGCAGCGACCTCATCCTGGAACGGGGCa                                                                                                                                       >  2:380092/1‑143 (MQ=255)
             tATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCa                                                                                                                                       >  2:939297/1‑143 (MQ=255)
                 ttcttTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCaa                                                                                                                                   >  2:803123/1‑143 (MQ=255)
                  tcttgccGCAGGCCCTTGCCGGTGCCTGCGCCAAACAGTTGTTCATCTTGAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTcc                                                                                                                            <  1:559953/143‑1 (MQ=255)
                    tttACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTACTCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGACAGCGACCTCACCCTGGAACGCGGCGTCAATAc                                                                                                                                >  2:436208/1‑143 (MQ=255)
                    gttACGCAGGCCCCTGCCGGTCCCTACGCCAAACAGTTTTTTATCTTTAACAGACGGGCCACCGAATTTGCAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCgg                                                                                                                          <  1:420492/148‑1 (MQ=255)
                       aCGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTCTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCCTCAATACGTCCGGcca                                                                                                                       >  1:881185/1‑147 (MQ=255)
                        cGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgt                                                                                                                      <  1:604781/149‑1 (MQ=255)
                          ccggCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCgg                                                                                                                          <  2:28742/141‑1 (MQ=255)
                          cAGGCCCATGCCTGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCgg                                                                                                                          <  2:439057/143‑1 (MQ=255)
                          cAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTATCAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCgg                                                                                                                          <  2:774067/143‑1 (MQ=255)
                               ccATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTGGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATCCGTCCGCCAgtcaggaca                                                                                                               >  1:450916/1‑149 (MQ=255)
                                cATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCGTGGACCGCGGCATCAATACGTCCGGCAgtcaggtcag                                                                                                              >  1:468814/1‑149 (MQ=255)
                                     cGGTCCCTACGCCAAACAGTTTTTCATTTTTATCAGGCGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAATCTTCGCTGGCAGCGGCCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggtca                                                                                                               <  2:894525/143‑1 (MQ=255)
                                     cGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGCATGTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGTGAGCGCCCTCCTCCTGGAACGCGGCATCCATCCGTCCGGCCgtcaggtca                                                                                                               >  2:542483/1‑143 (MQ=255)
                                     cGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAgtcaggcta                                                                                                               >  2:1145214/1‑140 (MQ=255)
                                      ggTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaaa                                                                                                        <  1:1158837/149‑1 (MQ=255)
                                            ttcGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTCTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCCATCCGTCCCGCAGTCAGGTCAGAATaaa                                                                                                        <  2:520814/141‑1 (MQ=255)
                                            tACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGaaaaaa                                                                                                        >  2:340382/1‑143 (MQ=255)
                                            tACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTAGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATaaa                                                                                                        <  2:276908/143‑1 (MQ=255)
                                              cGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtg                                                                                                      <  2:441797/143‑1 (MQ=255)
                                              cGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCGTTACCGACAGGTTGTTTGAGGAAACCTGCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAtg                                                                                                      <  2:655745/143‑1 (MQ=255)
                                                 cAAACAGTTTTTCATCTTTAACAGACGGGCCACACATCTAGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGc                                                                                             <  1:358509/149‑1 (MQ=255)
                                                   aaCAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGccc                                                                                           <  1:1066941/149‑1 (MQ=255)
                                                     cAGTTTTTCATCTTTACCAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCt                                                                                               <  2:613455/143‑1 (MQ=255)
                                                     cAGTTTTTCATCTTTAACAGACGGGCCACCGAACTAGTAATCTTTACCGACAGGTTGTTTGACGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCt                                                                                         <  1:883045/149‑1 (MQ=255)
                                                         ttttCATCTTTAACAGTCGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGccc                                                                                           <  2:883556/143‑1 (MQ=255)
                                                         ttttCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGccc                                                                                           <  2:461359/143‑1 (MQ=255)
                                                            tCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACga                                                                                  <  1:1068323/149‑1 (MQ=255)
                                                             cATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCATAATAAATGTTGTCCTGCCCCTGa                                                                                       <  2:381393/143‑1 (MQ=255)
                                                               tCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAGCCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGAc                                                                               <  1:1216388/149‑1 (MQ=255)
                                                               tCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATa                                                                                     <  2:643887/143‑1 (MQ=255)
                                                               tCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCACCTGATACGAGAc                                                                               >  1:330600/1‑149 (MQ=255)
                                                               tCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTCGAGGAAGCCTTCGCTTGCAGCTACCACAGCCTGGAACGCGGCATCAAGACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGAc                                                                               <  1:973048/149‑1 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTGAGATCAGAATAAATGTTGTGCTGCCCCTGATTCg                                                                                   >  2:216361/1‑143 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGa                                                                             >  1:924810/1‑149 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGa                                                                             >  1:774698/1‑149 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTAATACg                                                                                   >  2:591116/1‑143 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGCAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGa                                                                             >  1:774779/1‑149 (MQ=255)
                                                                 tttAACAGACGGGCCACCGAATTTGCAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGGCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGa                                                                             >  1:775285/1‑149 (MQ=255)
                                                                  ttAACAGCCGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAt                                                                            <  1:163479/149‑1 (MQ=255)
                                                                       aGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCaa                                                                       >  1:195081/1‑149 (MQ=255)
                                                                       aGACGGGCCACCGAATTTGTAATCTTTACCGACAAGATGGTTGAGGAAACCTTCGCTGGCAGCGACCTCGTCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATGCGAGACGa                                                                             >  2:805797/1‑143 (MQ=255)
                                                                       aGACGGGCAACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCGCATCCTGGAACGCGGCAGCAATACGTCCGGCAGGCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACgaagcga                                                                             >  2:920447/1‑138 (MQ=255)
                                                                          cGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAttt                                                                          <  2:947811/143‑1 (MQ=255)
                                                                               cACCGAATTTGTAATCTTTACCGACAGGATGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGGACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAGGTTGTCCAGCCCCTGGTACGAGACGATTACAATg                                                                     >  2:630123/1‑143 (MQ=255)
                                                                                aCCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGc                                                                    >  2:1032930/1‑143 (MQ=255)
                                                                                aCCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTg                                                             <  1:1126103/149‑1 (MQ=255)
                                                                                 ccGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCACAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGcc                                                                   >  2:742828/1‑143 (MQ=255)
                                                                                  cGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCt                                                                  >  2:291495/1‑143 (MQ=255)
                                                                                  cGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGt                                                           >  1:172133/1‑149 (MQ=255)
                                                                                    aaTTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGc                                                         <  1:1032220/149‑1 (MQ=255)
                                                                                       ttGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCa                                                      >  1:1300094/1‑149 (MQ=255)
                                                                                       ttGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTAGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCATGCCCCTGATACGAGACGAGTTCAATGCCTTTTgg                                                            >  2:612138/1‑143 (MQ=255)
                                                                                          tAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGAGTTTAATGCTCTTTGGTGc                                                         >  2:96545/1‑143 (MQ=255)
                                                                                          tAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTACGGCCGTCAAGTCAGAAGAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGc                                                         >  2:123398/1‑143 (MQ=255)
                                                                                           aaTCCTCACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGTCCGAATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAAGGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGt                                                  <  1:498294/149‑1 (MQ=255)
                                                                                            aTCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCCATGtt                                                 >  1:1151255/1‑149 (MQ=255)
                                                                                             tCTTTACCGACAGGTTGTTTTAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCCGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCa                                                      <  2:1137764/143‑1 (MQ=255)
                                                                                             tCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCGGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTc                                                <  1:800742/149‑1 (MQ=255)
                                                                                                    cGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCg                                         >  1:238914/1‑149 (MQ=255)
                                                                                                      aCAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGGTACGAGACGATTTCGATGCCTTTTGGTGCCCAATGTTCATTACCgga                                       >  1:357814/1‑147 (MQ=255)
                                                                                                       cAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCAGTCCCTGATTCGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAAc                                      <  1:255260/149‑1 (MQ=255)
                                                                                                           ttgttTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGtct                                  >  1:1111810/1‑149 (MQ=255)
                                                                                                              tttGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTGATTACGGAACg                                     >  2:239094/1‑143 (MQ=255)
                                                                                                              tttGAGGAAACCTTCGCTGGCAGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCt                               <  1:309910/149‑1 (MQ=255)
                                                                                                               ttGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCGGATACGAGACGATTTCAATTCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTg                              >  1:1021852/1‑149 (MQ=255)
                                                                                                                 gAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGtct                                  >  2:563814/1‑143 (MQ=255)
                                                                                                                  aGGAAACCTTCGCTGGCAGCGCCCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGACTTCACTGCCTTTTGGCGCCCAATGTTCATTACCGAACGtctc                                 <  2:873986/143‑1 (MQ=255)
                                                                                                                  aGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGACCGTGTCCttgct                           >  1:1259934/1‑145 (MQ=255)
                                                                                                                   ggAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTcc                                >  2:90588/1‑143 (MQ=255)
                                                                                                                         cgtggccggCCGCGACCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGGCGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGggtg                          <  2:67395/136‑1 (MQ=255)
                                                                                                                         cTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGggtg                          <  2:72727/143‑1 (MQ=255)
                                                                                                                         cTTCGCTGCCAGCGCCCTCACCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGggtg                          <  2:674970/143‑1 (MQ=255)
                                                                                                                              cTGGCAGCGACCTCCTCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCaa               <  1:862851/149‑1 (MQ=255)
                                                                                                                                ggCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCt                   <  2:1152620/143‑1 (MQ=255)
                                                                                                                                 gCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGCTTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATAc            >  1:648075/1‑149 (MQ=255)
                                                                                                                                 gCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATAc            >  1:519450/1‑149 (MQ=255)
                                                                                                                                   accGACCTCATCCTGGAACCCGGCATCACACCGTCCGGCAGTGAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGGGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGc          <  1:564505/147‑1 (MQ=255)
                                                                                                                                   aGCGACCTCATGCTGGAACGCGGCATCAATCCGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCa                <  2:967876/143‑1 (MQ=255)
                                                                                                                                   aGCGAAGTCAGCCGGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATGAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCa                >  2:1112661/1‑143 (MQ=255)
                                                                                                                                     cGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCACTGAATATTTGGTCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCt        >  1:1273477/1‑149 (MQ=255)
                                                                                                                                     cGACCTCATCCTGGAACGCGGCATCAATACGTCCGACAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTGCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAAt              >  2:1118030/1‑143 (MQ=255)
                                                                                                                                      gACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATa             >  2:658775/1‑143 (MQ=255)
                                                                                                                                           cATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGt  <  1:970751/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
TCGCGCAGTTCGTTATCTTCTTTACGCAGGCCCATGCCGGTCCCTACGCCAAACAGTTTTTCATCTTTAACAGACGGGCCACCGAATTTGTAATCTTTACCGACAGGTTGTTTGAGGAAACCTTCGCTGGCAGCGACCTCATCCTGGAACGCGGCATCAATACGTCCGGCAGTCAGGTCAGAATAAATGTTGTCCTGCCCCTGATACGAGACGATTTCAATGCCTTTTGGTGCCCAATGTTCATTACCGAACGTCTCCTGGGTGGTGCCCTGCAATACGCCTACCCGT  >  CP009273/2419579‑2419866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: