Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,420,353 A→T 58.7% intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,420,3530AT58.7% 47.0 / 94.8 84intergenic (‑86/+135)hisJ/argThistidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (24/10);  new base T (29/20);  total (54/30)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

ACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGA  >  CP009273/2420207‑2420501
                                                                                                                                                  |                                                                                                                                                    
aCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAatat                                                                                                                                                    <  1:97631/149‑1 (MQ=255)
    cgtaGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTTTAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATcc                                                                                                                                                <  1:648313/146‑1 (MQ=255)
      cAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATGTTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCg                                                                                                                                              >  1:558281/1‑149 (MQ=255)
          tGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATcc                                                                                                                                                <  2:164121/143‑1 (MQ=255)
          tGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATcc                                                                                                                                                <  2:943532/143‑1 (MQ=255)
               tGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGttgg                                                                                                                                     >  1:588599/1‑147 (MQ=255)
                    aaGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATGCAGGCAATATATGCTGACCCGTTCGACgga                                                                                                                                >  1:237514/1‑147 (MQ=255)
                    aaGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACgaa                                                                                                                                >  1:588910/1‑147 (MQ=255)
                       gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATGTTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTGGACGtatta                                                                                                                             >  1:352063/1‑149 (MQ=255)
                       gCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACAATTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtatta                                                                                                                             >  1:1147203/1‑149 (MQ=255)
                              ccAGAGAGAGCGACAGCACCAGTTTTCTCAGAAGGTCCTCAATTCAGCAAATATTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCa                                                                                                                      <  1:298047/149‑1 (MQ=255)
                                  agagagCGAGAGCACCATTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAAATTCATTACACTCAGCAGTCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTACACGTATTAttttt                                                                                                                        <  2:1013/143‑1 (MQ=255)
                                    agagCGATAGCACCAGTTTTTTCATAATGGCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCa                                                                                                                      <  2:22542/143‑1 (MQ=255)
                                      agCGATAGCACCAGTTTTTTCATTTTTTCCTCAACTGACAGTCTCTTTATAGGGAATATTTACAGACTTGGTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACTCATTACTTTTCAtc                                                                                                                    >  2:18090/1‑142 (MQ=255)
                                      agCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAt                                                                                                              >  1:524983/1‑149 (MQ=255)
                                      agCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAt                                                                                                              >  1:405512/1‑149 (MQ=255)
                                      agCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTGTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATCTTTCAtt                                                                                                                    >  2:712563/1‑143 (MQ=255)
                                        cGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGc                                                                                                            >  1:581061/1‑149 (MQ=255)
                                            aGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAt                                                                                                              <  2:142840/143‑1 (MQ=255)
                                              cACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGt                                                                                                      >  1:1174380/1‑149 (MQ=255)
                                                ccAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTAt                                                                                                    >  1:1058794/1‑149 (MQ=255)
                                                 cAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTa                                                                                                         >  2:1062620/1‑143 (MQ=255)
                                                 cAGTTTTTTCATAATGTCCTCAACTGACAGTATGTTTATAGGGAATATTTACAGGTATGTTACATTTATCGTGCAATAAAATTGAACATCAAGGCACTATATCCCGCCCCGTTAGACGTACTATTTTTCCTTATAAATgttca                                                                                                         >  2:179546/1‑139 (MQ=255)
                                                  aGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTTTTGGTAATATTTACAGGTTTGTTACACTTATCGTGCTATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGc                                                                                                  <  1:38728/149‑1 (MQ=255)
                                                  aGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGc                                                                                                  <  1:1038581/149‑1 (MQ=255)
                                                   gTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTCTCGTGCCATAAAATTGACCATCAAGGCAAAATATGCCGCCCCGTGGGACGTATTGTTTTTCATTAAAAATACTTatg                                                                                                       >  2:966129/1‑141 (MQ=255)
                                                       tttCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGAATGCAtctc                                                                                             >  1:587744/1‑149 (MQ=255)
                                                            tAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACATGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTCTTTTTCATTACAAATGCCTACTTATGAAttt                                                                                              >  2:495345/1‑143 (MQ=255)
                                                            tAATGTCCTCAACTGACAGTCTTATTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGc                                                                                        >  1:722754/1‑149 (MQ=255)
                                                             aaTGTCCTCAACTGACAGGCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAACATATCCCCCCCCGCTAGACGTATTATTTCTCATTCAAAATGCTTAAGTATGCATTTCAATGca                                                                                       >  1:414818/1‑149 (MQ=255)
                                                              aTGTCCTCAACTGATATTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGcac                                                                                      <  1:165677/149‑1 (MQ=255)
                                                               tGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCCAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAAGGCTTAAGTATGCAGTTCaa                                                                                           >  2:946506/1‑143 (MQ=255)
                                                                tcccTCCACTGACATTCTTTTTATAGGGAATATTTACCGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTAGTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGcacca                                                                                    <  1:1288874/147‑1 (MQ=255)
                                                                gTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTCAGTATGCATTTCCATGcccca                                                                                    >  1:435486/1‑149 (MQ=255)
                                                                gTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCACCGTTAGACGTATTATTTTTCATTAAAAATGCTTAGGTATGCATTTCAACGcacca                                                                                    >  1:435149/1‑149 (MQ=255)
                                                                 tCCTCAACTGACAGTCTTTTTATAGGGAATATTTCCAAGTCGGTCACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGcaccac                                                                                   >  1:1156701/1‑149 (MQ=255)
                                                                 tCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATg                                                                                         <  2:55096/143‑1 (MQ=255)
                                                                   cTCAACTGACAGTCTTCTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGACATAAAATTGACCATCAATGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGca                                                                                       >  2:148931/1‑143 (MQ=255)
                                                                    tCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGAt                                                                                >  1:881674/1‑149 (MQ=255)
                                                                     cAAGTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATGGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATa                                                                               <  1:807958/149‑1 (MQ=255)
                                                                      aaCTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGcacca                                                                                    >  2:2005/1‑143 (MQ=255)
                                                                       aCTGACAGTCTTTTTATAGGGAATATTTCCAGGTTTGTTACACTGATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGt                                                                             <  1:1120036/149‑1 (MQ=255)
                                                                        cTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACGTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTg                                                                            <  1:1187475/149‑1 (MQ=255)
                                                                           ataGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATa                                                                               <  2:161756/141‑1 (MQ=255)
                                                                           aCAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATa                                                                               <  2:930382/143‑1 (MQ=255)
                                                                            cAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACCCTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAg                                                                              <  2:21274/143‑1 (MQ=255)
                                                                             aGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGt                                                                             >  2:417306/1‑143 (MQ=255)
                                                                             aGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaaca                                                                       >  1:1262690/1‑149 (MQ=255)
                                                                               tCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCaaaaaa                                                                     >  1:7653/1‑149 (MQ=255)
                                                                               tCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACaaa                                                                     >  1:1055193/1‑149 (MQ=255)
                                                                                 tttttGTAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAACATGGACCATCAAGGCAAAATATCCCGCCCCGTTAGTCGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaa                                                                         <  2:26255/143‑1 (MQ=255)
                                                                                 tttttATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAcc                                                                   >  1:1197926/1‑149 (MQ=255)
                                                                                 tttttATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAccc                                                                   >  1:765585/1‑149 (MQ=255)
                                                                                 ttcttATAGTGAATATTTACAGGTTTCTTACCCTTATCGTGCCATAAAATTGACCATCACGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGcaa                                                                         <  2:371302/140‑1 (MQ=255)
                                                                                      ataGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAc                                                                    >  2:286777/1‑143 (MQ=255)
                                                                                              tatTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCat                                                      >  1:360308/1‑149 (MQ=255)
                                                                                                 ttccAGGTGTGTCACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCAc                                                         <  2:79140/140‑1 (MQ=255)
                                                                                                 ttACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCCTGCAc                                                         >  2:420702/1‑143 (MQ=255)
                                                                                                   accagtgtgtgaaacTTAACGTGCCATAAAACTAACCATCAAGGCAAAATATCCGGGATGGTGGGAGGTAATAAAAAAAAAAAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCa                                                       <  2:979359/130‑1 (MQ=255)
                                                                                                      ggTTTGTTACACTTATCGTGCTATATAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATTCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCa                                              <  1:858488/149‑1 (MQ=255)
                                                                                                       gTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCatgat                                                   >  2:56279/1‑143 (MQ=255)
                                                                                                       gTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAAACTGGCGTGCACCatgct                                                   >  2:548041/1‑141 (MQ=255)
                                                                                                        tttGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGGTAGACGTAGTATTTTTCATTAAAAATGCTTCAGTCTGCATGTCGTTGCACCGCGATGTTAACACCAACCTCGCCTGCACCatgatg                                                  >  2:590621/1‑143 (MQ=255)
                                                                                                         ttGATAGACTTACCGACCCAGAACATTGACCATCAAGGAAATATACCCCGCCCCGTTAGACGTATAAAAAAAAAATAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCAtgatga                                                 <  2:227426/143‑1 (MQ=255)
                                                                                                              acacTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGGCGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCa                                      >  1:1054996/1‑149 (MQ=255)
                                                                                                              acacTTATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCAtt                                            >  2:51652/1‑143 (MQ=255)
                                                                                                                 cTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAGACCTGGCGTGCACCATGATGATCATTCCATCAGGt                                   >  1:723925/1‑149 (MQ=255)
                                                                                                                  ttATCGTGCCATAAAATTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCAt                                        >  2:742036/1‑143 (MQ=255)
                                                                                                                   tATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTAc                                 <  1:385244/149‑1 (MQ=255)
                                                                                                                    accgggCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCa                                      <  2:48337/138‑1 (MQ=255)
                                                                                                                          ccATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGGAACAAGCCTGGCGTGCACCATGATCGTCATTCCAtacgggaca                                >  2:574162/1‑135 (MQ=255)
                                                                                                                             tAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAg                       >  1:886247/1‑149 (MQ=255)
                                                                                                                                aaTTGACCATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCTTCTTGTGAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGa                    >  1:949955/1‑149 (MQ=255)
                                                                                                                                aaTTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCtttcgacgga                    >  1:140478/1‑141 (MQ=255)
                                                                                                                                 aTTGACCATCAAGGCAATATACCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGAc                   <  1:854677/149‑1 (MQ=255)
                                                                                                                                  ttGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTcccc                        >  2:694874/1‑142 (MQ=255)
                                                                                                                                    gACCATCAAGGCAATATATCCCGCCTCGTTAGACTGATTGTTTTTCATTAAAAATGCTTAAGTATGCATTGCAATGCACCACGATTGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGc                      <  2:891220/143‑1 (MQ=255)
                                                                                                                                      acactaaGGAAATATATCCCGCCCCGTTAGACGTATTCTTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGa                    <  2:575309/138‑1 (MQ=255)
                                                                                                                                       cATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACCAACCTGGCGTGCACCATGATGATCATTCCATCAGGGACAGCCTCCCAGCGAc                   >  2:1115480/1‑143 (MQ=255)
                                                                                                                                       cATCAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGAACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCa             >  1:451903/1‑149 (MQ=255)
                                                                                                                                          cAAGGCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTc          >  1:1237146/1‑149 (MQ=255)
                                                                                                                                             ggCAAAATATCCCGACCCGTGAGACGTATTACTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAATCCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCa             <  2:1119709/143‑1 (MQ=255)
                                                                                                                                              gCAATATATCCCGCCCCGTTAGACGTGTTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTGTCAg            >  2:557809/1‑143 (MQ=255)
                                                                                                                                              gCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGGCGGATCAg            >  2:632915/1‑143 (MQ=255)
                                                                                                                                              gCAATATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCg      >  1:48023/1‑149 (MQ=255)
                                                                                                                                                aaaaTATCCCGCCCCGTTAGACGTATTTTTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTTCACCATGATGATCCTTCCATCAAGTCTACCTTCCCTGCGAGGTATCTGTGACCATa    >  1:164462/1‑149 (MQ=255)
                                                                                                                                                 aaaTATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAg   >  1:1270991/1‑149 (MQ=255)
                                                                                                                                                 aaaTATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAg   >  1:1222106/1‑149 (MQ=255)
                                                                                                                                                  aaTATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCTTGCACCATGATGATCACTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGa  <  1:364820/149‑1 (MQ=255)
                                                                                                                                                  aaTATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGa  <  1:1182477/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGTATGCATTTCAATGCACCACGATAGTGCAACAAACCTGGCGTGCACCATGATGATCATTCCATCAGGTACAGCTTCCCAGCGACGTATCAGTCACCGTAGA  >  CP009273/2420207‑2420501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: