BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,524,024	C→T	3.8%	R190H (CGC→CAC)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,524,024	0	C	T	3.8%	315.5 / 2.3	107	R190H (CGC→CAC)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base C (50/51); new base T (2/2); total (52/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01

Only 100 of 117 total aligned reads displayed.

GCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACT  >  CP009273/2523876‑2524170

     tGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTc                                                                                                                                               >  1:383197/1‑149 (MQ=255)

      gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCg                                                                                                                                              <  1:1218882/149‑1 (MQ=255)

      gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCg                                                                                                                                              <  1:552125/149‑1 (MQ=255)

      gCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACAGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTgc                                                                                                                                                    <  2:736111/143‑1 (MQ=255)

       ccATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCGAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTgcg                                                                                                                                                   >  2:244036/1‑143 (MQ=255)

       ccATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGGTAATCACCGCTTCTTTgcg                                                                                                                                                   >  2:919945/1‑143 (MQ=255)

           ccGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTa                                                                                                                                         <  1:568350/149‑1 (MQ=255)

                cTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCGTCGGTTTATCCAt                                                                                                                                    >  1:329247/1‑149 (MQ=255)

                   aGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGATGATACTTAGCTGTTTCCCGATGCATCCGCTACCGTGATGCGCCTCCATGATCGTGATAATCACCGCTTCTTTTCGCTTCGGTTTATCCATAAc                                                                                                                                 >  1:1280624/1‑149 (MQ=255)

                     aTGACGATGGTAAAAATTCGTATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGGTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTg                                                                                                                               <  1:644587/149‑1 (MQ=255)

                      tGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTgg                                                                                                                              >  1:710536/1‑149 (MQ=255)

                       gACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGCCGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATa                                                                                                                                   >  2:1009671/1‑143 (MQ=255)

                       gACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGGTGAATGCTGTTAAGAAGCAGCATGTCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGa                                                                                                                             >  1:1218185/1‑149 (MQ=255)

                              gTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGtt                                                                                                                      <  1:1276930/149‑1 (MQ=255)

                              gTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGGTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCGTCTTTGCGCTTCGGTTTATACATAAcatgcg                                                                                                                            >  2:76377/1‑138 (MQ=255)

                                aaaTATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATCACTGGAGCa                                                                                                                          >  2:582043/1‑143 (MQ=255)

                                           tCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATCATCTACGCTTCTTTGAGATTTGGTTCATCCATAACTGGATCAGGTgctgcttt                                                                                                               <  2:659811/143‑3 (MQ=255)

                                           tCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCtcc                                                                                                               >  2:152492/1‑141 (MQ=255)

                                            cGCCAAAAGTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCTGCTTca                                                                                                              <  2:1070360/143‑1 (MQ=255)

                                             gCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAcaggctcag                                                                                                       >  1:993560/1‑149 (MQ=255)

                                               cAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAcaggctcaggc                                                                                                     >  1:1261883/1‑149 (MQ=255)

                                                aaaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAcaggctcaggct                                                                                                    >  1:904136/1‑149 (MQ=255)

                                                 aaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCGGCTCcaggctc                                                                                                         >  2:353722/1‑143 (MQ=255)

                                                  aaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAcaggctca                                                                                                        >  2:605495/1‑143 (MQ=255)

                                                   aaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCtgtg                                                                                                 >  1:1270843/1‑149 (MQ=255)

                                                        aGCCCGCTTGTTGAACGCTGTTAAGAAGCAGTTCACCGTTTAGTTCGCTACCGTGATGCCCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGcc                                                                                            <  1:860822/149‑1 (MQ=255)

                                                        aGCCCGCTTGGTGTAGGCTGCTAAGAAGCAGTTCACCGTTGAGCTCGCTCCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGcc                                                                                            <  1:1064996/149‑1 (MQ=255)

                                                         gCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCg                                                                                           >  1:1193408/1‑149 (MQ=255)

                                                           acGCTTGTTGAATGCTGTTAAGAAGCAGTTCGCCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTgtggt                                                                                               >  2:832035/2‑143 (MQ=255)

                                                            cGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGGTCCGCTACAGGCTCAGGCTgtggtg                                                                                              <  2:808627/143‑1 (MQ=255)

                                                            cGCTTGTTGAACGCTGTTAAGAAGCAGTGCACCGTTTAGCTCGCTACCCTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCCTAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTa                                                                                        <  1:649736/149‑1 (MQ=255)

                                                                 gttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCCCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGt                                                                                   >  1:1225087/1‑149 (MQ=255)

                                                                  ttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCGTAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGcgggtg                                                                                        >  2:653745/1‑138 (MQ=255)

                                                                  ttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTTCCGCTACGGGtt                                                                                  >  1:814973/1‑149 (MQ=255)

                                                                     aatgtggTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACgg                                                                                     <  2:379960/137‑1 (MQ=255)

                                                                     aaggttGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACgg                                                                                     <  2:379867/138‑1 (MQ=255)

                                                                     aaTGCTGTTAAGAGGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTGCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTg                                                                               >  1:921272/1‑149 (MQ=255)

                                                                      aggCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGc                                                                              <  1:38739/147‑1 (MQ=255)

                                                                        gCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGtt                                                                                  >  2:243017/1‑143 (MQ=255)

                                                                          tGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCAGAATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGc                                                                          >  1:255630/1‑149 (MQ=255)

                                                                           gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTg                                                                               >  2:337876/1‑143 (MQ=255)

                                                                           gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCt                                                                         >  1:105599/1‑149 (MQ=255)

                                                                               agaagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGCTTATCCATACCTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAgg                                                                           <  2:101618/143‑1 (MQ=255)

                                                                                caagtcgTTCACCGTATAGCTCGCTACCGTAATGCGCCGCGACGTTCATGATAATCACCTCCTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGTCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGaaa                                                                    <  1:89302/143‑1 (MQ=255)

                                                                                 aagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCt                                                                         >  2:694378/1‑143 (MQ=255)

                                                                                 aagCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGCTCAGGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGGGGTGCCGCTACGGGTTCTGCAGGCTGGAAAg                                                                   >  1:838318/1‑149 (MQ=255)

                                                                                  agCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTGCAGTCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGc                                                                  <  1:631361/149‑1 (MQ=255)

                                                                                  agCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCGGGCTGGAAAGc                                                                  <  1:1247939/149‑1 (MQ=255)

                                                                                   gCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTgg                                                                       <  2:672247/143‑1 (MQ=255)

                                                                                   gCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTGCTGCAGGCTGGAAAGcc                                                                 >  1:40858/1‑149 (MQ=255)

                                                                                    cAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGATCCGCTACAGGCACAGGCTGTGGTGCCGCTACGGGTCCTGCAGGCTGGa                                                                      >  2:490027/1‑143 (MQ=255)

                                                                                     acttCACCGTTTAGCTCGCCACCGTGACGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGGTCTGCAGGCTGGaa                                                                     <  2:574660/141‑1 (MQ=255)

                                                                                     aGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtg                                                               <  1:49141/149‑1 (MQ=255)

                                                                                     aGTCCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGATCATTACAGCCTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtg                                                               <  1:304632/149‑1 (MQ=255)

                                                                                       ttCACCGTTTAGCCCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGCGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgtt                                                             <  1:652430/149‑1 (MQ=255)

                                                                                            cGTTTGGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGcc                                                        <  1:403982/149‑1 (MQ=255)

                                                                                            cGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGcc                                                        <  1:1067255/149‑1 (MQ=255)

                                                                                              tttAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCCGGCTGTGGGGCCGCTACGGGTTCGGCAGGCTGGAAAGCCtgttg                                                            >  2:564372/1‑143 (MQ=255)

                                                                                                tAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGtt                                                    <  1:427651/149‑1 (MQ=255)

                                                                                                   cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGTGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                 <  1:1233652/149‑1 (MQ=255)

                                                                                                   cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGTGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                 <  1:1233297/149‑1 (MQ=255)

                                                                                                   cTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCCACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                 <  1:689573/149‑1 (MQ=255)

                                                                                                   cTCGCTACCGTGATGCCCAGCGACGCTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAACCTGTAGTGccc                                                       >  2:495330/1‑142 (MQ=255)

                                                                                                    tCGCTCCCGTGGTGCGCCGCGACGTTCATGATAACCACCGCTTCTTTGCGCCTCGGTTTATCCGTAACGGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCCACGGGTGCTGCAGGCCGGGAAGCCTGTTGTGCCgg                                                      >  2:576891/1‑143 (MQ=255)

                                                                                                    tCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGTGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCgg                                                      >  2:1091006/1‑143 (MQ=255)

                                                                                                    tCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGTGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCgg                                                      >  2:1090655/1‑143 (MQ=255)

                                                                                                     cGCTCCCGGGAGGCGCCGCTACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGt                                                     <  2:714322/143‑1 (MQ=255)

                                                                                                          ccGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGCTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGa                                          <  1:1201447/149‑1 (MQ=255)

                                                                                                          ccGTGATGCGCCGCGACCTTCATGATAATCCCCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGa                                          <  1:1069579/149‑1 (MQ=255)

                                                                                                           cGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGt                                               >  2:609218/1‑143 (MQ=255)

                                                                                                           cGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGaa                                         >  1:532041/1‑149 (MQ=255)

                                                                                                           accagctggccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCCGGCTGGACAGCCTGTTGTGCCGGTTGCGGt                                               >  2:698736/9‑143 (MQ=255)

                                                                                                                tGCGCCGCGACGTTCATGAGAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCCGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGa                                          <  2:1127892/143‑1 (MQ=255)

                                                                                                                ggcgcCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGcac                                    <  1:205679/148‑1 (MQ=255)

                                                                                                                   gccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTGATCCATAACTGTAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATg                                       <  2:1083142/143‑1 (MQ=255)

                                                                                                                         aCGTTCATGATAACCGCCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcgg                           <  1:391287/149‑1 (MQ=255)

                                                                                                                           gTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcggcg                         >  1:87378/1‑149 (MQ=255)

                                                                                                                            gtCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                              >  2:839991/2‑143 (MQ=255)

                                                                                                                              cATGATAATCCCCGCTGCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcg                            <  2:610658/143‑1 (MQ=255)

                                                                                                                                  aTAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGTTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                        <  2:410263/143‑1 (MQ=255)

                                                                                                                                  aTAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                        <  2:1076712/143‑1 (MQ=255)

                                                                                                                                  aTAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGGGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                        <  2:375814/143‑1 (MQ=255)

                                                                                                                                    aaTCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                      <  2:1116750/143‑1 (MQ=255)

                                                                                                                                     aTCACCGCTTCTTTGCGCTTCGGTTCATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTCCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc               <  1:802971/149‑1 (MQ=255)

                                                                                                                                      tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCCCCGGCTGCGGCGCTGgc                    >  2:470326/1‑143 (MQ=255)

                                                                                                                                      tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGgc                    <  2:537690/143‑1 (MQ=255)

                                                                                                                                      tCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGgc                    >  2:49634/1‑143 (MQ=255)

                                                                                                                                        aCCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtg            >  1:294454/1‑149 (MQ=255)

                                                                                                                                          cGCTTCTTTGCGGTTCGGTTTAGCGATAACTGGAGCAGGTTCCGCTACAGTTTCAGGCTGTGGTGCCGCGACGGGTTCTGCAGGTTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGa                <  2:216884/143‑1 (MQ=255)

                                                                                                                                           gCTTCTGTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGAc               <  2:725288/143‑1 (MQ=255)

                                                                                                                                                tttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGTTGCCGCTGCGGGTTTTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGGCCtgtg          >  2:837633/1‑143 (MQ=255)

                                                                                                                                                tttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaaa    <  1:1260110/149‑1 (MQ=255)

                                                                                                                                                 ttccgcTTCGGTTGATCCAGAACTGGAGCAGGTTCCGCCCCAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGGTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTgg         <  2:378159/140‑1 (MQ=255)

                                                                                                                                                 ttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTAGAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAGAGCCTGTTGTGCCGGTTGCGGTGCTGAATTCACGGGCTGCGGCGCTGGCGCGGCCTGTGGCGaacc   >  1:139296/1‑147 (MQ=255)

                                                                                                                                                  tacgcTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGTTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGc        >  2:142267/3‑143 (MQ=255)

                                                                                                                                                  tGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGc        <  2:206311/143‑1 (MQ=255)

                                                                                                                                                  tGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACt  >  1:300293/1‑149 (MQ=255)

                                                                                                                                                  tGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTGCTGCAGGCTGGAAAGGCTGTTGTGCCGGTTGCGGTACTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACt  >  1:993419/1‑149 (MQ=255)

                                                                                                                                                   gcgcTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCg       >  2:819735/1‑143 (MQ=255)

                                                                                                                                                    cgcTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCGGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGGGGCGa      >  2:577800/1‑143 (MQ=255)

GCCGCTGCCATCCGGGCTAAGATGACGATGGTAAATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACT  >  CP009273/2523876‑2524170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`