Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,861,917 G→A 40.6% Q66* (CAG→TAG)  nlpD ← activator of AmiC murein hydrolase activity, lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,861,9170GA40.6% 58.1 / 111.8 109Q66* (CAG→TAG) nlpDactivator of AmiC murein hydrolase activity, lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (33/31);  new base A (18/26);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 113 total aligned reads displayed.

TTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGAC  >  CP009273/2861769‑2862065
                                                                                                                                                    |                                                                                                                                                    
ttCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTtgctg                                                                                                                                                      >  1:1147157/1‑149 (MQ=255)
    gAATGTTCCCTACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTCAATTTGTGGCTGCTGCAACGGCTGAATTTGCGGTTGCTGCGCTg                                                                                                                                                 <  1:685771/149‑1 (MQ=255)
       tGTTCCCATACTGACGGTTATAGGCGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGGTGTTGGGTAGCCTGAATTTGTGTCTGCTGCGCGCGCTGATTTTGCGGTtgctgc                                                                                                                                                     >  2:760697/1‑143 (MQ=255)
         ttCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGa                                                                                                                                             <  1:226567/149‑1 (MQ=255)
          tCCCAGACTGCCGGGTATAGCCGATGCGTCCGTTTTCCATCTGTCCCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCt                                                                                                                                                  <  2:555053/143‑1 (MQ=255)
           cccATACTGACGGTTATCGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCCGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGAcg                                                                                                                                           <  1:231337/149‑1 (MQ=255)
           cccATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGCcg                                                                                                                                           >  1:1023915/1‑149 (MQ=255)
            ccATCCTGGCGGTTTGTGGCGATGCGTCCGTTTTCCCTCGGTCCCGGCTGCTGAGCTATTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGAcgt                                                                                                                                          <  1:480979/149‑1 (MQ=255)
            ccATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGt                                                                                                                                                >  2:305028/1‑143 (MQ=255)
            ccATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGt                                                                                                                                                >  2:1079750/1‑143 (MQ=255)
              aTACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGAcgtcg                                                                                                                                        >  1:612005/1‑149 (MQ=255)
              aTACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAg                                                                                                                                              >  2:372301/1‑143 (MQ=255)
              aTACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAg                                                                                                                                              >  2:53255/1‑143 (MQ=255)
               tACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTCCCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGAcgtcgt                                                                                                                                       <  1:698940/149‑1 (MQ=255)
               tACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGCGCTACTCGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCCGCTGCACCGGCCGAATTTGCGGTTGCTGCGCTCCGGAcgtcgg                                                                                                                                       >  1:122279/1‑148 (MQ=255)
               tACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGCGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGa                                                                                                                                             <  2:746641/143‑1 (MQ=255)
                 cTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTcc                                                                                                                                     <  1:251112/149‑1 (MQ=255)
                 cTGACGGTTATAGACGATGCGGCCGTTTTCCATCTGTACCGGTTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGAcg                                                                                                                                           <  2:782313/143‑1 (MQ=255)
                  tGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGAcgt                                                                                                                                          >  2:1148117/1‑143 (MQ=255)
                    aCGGTTATAGGCGGTGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGAcgtcg                                                                                                                                        <  2:409368/143‑1 (MQ=255)
                    aCGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCa                                                                                                                                  <  1:1027818/149‑1 (MQ=255)
                      ggTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCAtt                                                                                                                                <  1:895877/149‑1 (MQ=255)
                       gTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGACCTGCGGTTGTTGAGTGGCCTGAACTCGTGGCTGCTGCACCGGCTGAATCTGCGGTTGCCGCGCTGTAGACGTCGTcc                                                                                                                                     >  2:1028024/1‑143 (MQ=255)
                        ttATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCAtttt                                                                                                                              >  1:463556/1‑149 (MQ=255)
                          ctaGACGAGGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCa                                                                                                                                  <  2:1094595/142‑1 (MQ=255)
                          agaGACGATGCTGCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCa                                                                                                                                  <  2:486489/141‑1 (MQ=255)
                            aGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTcggc                                                                                                                          >  1:182628/1‑149 (MQ=255)
                                gATGCGTCCGTTTTCCATCCGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTcggcggcg                                                                                                                      <  1:1062909/149‑1 (MQ=255)
                                     gTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTa                                                                                                                 >  1:64911/1‑149 (MQ=255)
                                      tCCGTTTTCCATCTGTACCGGCTGCTGAGGTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGTGTAGCCTGCATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTATcggcggcg                                                                                                                      >  2:510922/1‑143 (MQ=255)
                                      tCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCGGTAGACGTCGTCCCCATTTTcggggggg                                                                                                                      >  2:17527/1‑141 (MQ=255)
                                      tCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTAGGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTaa                                                                                                                >  1:376716/1‑149 (MQ=255)
                                      tCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCGGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTaa                                                                                                                <  1:869283/149‑1 (MQ=255)
                                       ccGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAAt                                                                                                               <  1:351165/149‑1 (MQ=255)
                                         gTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAGTcg                                                                                                             >  1:421322/1‑148 (MQ=255)
                                                 tCTGTACCGGCTGCTGGGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAAc                                                                                                           <  2:787858/143‑1 (MQ=255)
                                                 tCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGCCGGCGTAATCACCAtccga                                                                                                     >  1:1128366/1‑145 (MQ=255)
                                                  cTGTACCGGCTGCTGAGCTCCCGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACa                                                                                                          <  2:613098/143‑1 (MQ=255)
                                                  cTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACa                                                                                                          <  2:584348/143‑1 (MQ=255)
                                                  cTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACa                                                                                                          <  2:297974/143‑1 (MQ=255)
                                                   tGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGa                                                                                                   <  1:172508/149‑1 (MQ=255)
                                                       ccGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCGCAATTa                                                                                               >  1:671882/1‑149 (MQ=255)
                                                       ccGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTa                                                                                               >  1:1189239/1‑149 (MQ=255)
                                                       ccGGCTGCTGAGCTACTGGCTGCACTGGCGGGATTTGCGGTTGTTGAGTAGCCTGAATTGGTGGCTGCTGCACCGGCTGAATTTGCGGGTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGGAATCAACATAccc                                                                                                     >  2:329644/1‑142 (MQ=255)
                                                       ccGGCTGCTGAGCTACTGGCTGCACTGACTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTa                                                                                               <  1:905747/149‑1 (MQ=255)
                                                        cGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAg                                                                                                    >  2:239217/1‑143 (MQ=255)
                                                              ctgAGCTAATGGCTGCACTGGCTGGATTTGCGGGTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTg                                                                                        >  1:951306/1‑149 (MQ=255)
                                                               tgtgCTTCTGGTTGCACTGGCTGGATTTGCGGGTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGc                                                                                       <  1:18983/146‑1 (MQ=255)
                                                                gggCTACTTGTTGCACGGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCGACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTa                                                                                            <  2:257041/141‑1 (MQ=255)
                                                                 aGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAg                                                                                     >  1:1091042/1‑149 (MQ=255)
                                                                 aGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAg                                                                                     <  1:829304/149‑1 (MQ=255)
                                                                 aGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAAACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTAt                                                                                           >  2:35023/1‑143 (MQ=255)
                                                                   cTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGCGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCACCATACCAGAATTATTAttt                                                                                         >  2:166239/1‑143 (MQ=255)
                                                                   cTACTGGCTGCACTGGCTGGATTTGCGGGTGTTGAGTAGCCTGAATTTGTGGCTGCTACACCGGCTGAATCTGCGGATGCTTCACTGTAGACATCGTCCGCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGgc                                                                                   >  1:973526/1‑149 (MQ=255)
                                                                      cTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCa                                                                                >  1:420324/1‑149 (MQ=255)
                                                                         gcggCACTGGCTGGATTTGCGGTTGTTGAGTGGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTg                                                                             <  1:1057198/146‑1 (MQ=255)
                                                                         gCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTg                                                                             >  1:1022227/1‑149 (MQ=255)
                                                                             cACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCAt                                                                               >  2:1132070/1‑143 (MQ=255)
                                                                             cACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCAt                                                                         <  1:472888/149‑1 (MQ=255)
                                                                             cACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCAt                                                                         <  1:761352/149‑1 (MQ=255)
                                                                             cACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGGTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCGGGCGCAt                                                                               >  2:1150786/1‑143 (MQ=255)
                                                                                tggctggATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCTCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTaa                                                                      >  1:879098/1‑149 (MQ=255)
                                                                                 ggctggACTTGTGGTTGTTGCGGTGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCGCCGGTGTGGTTTTAGTATTGGCAGGCGCATTGcc                                                                           <  2:246601/143‑1 (MQ=255)
                                                                                   ctggATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACgg                                                                   >  1:741507/1‑149 (MQ=255)
                                                                                    ttgATTTGCAGTCGTTGAGTAGCCTTAATTTGTCGCTGCTGCACCGACTGAATTTGCGGTTGCTGCGCTTTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAACCAACATACCAGAATTAGTATTTGCACGCGCATTGCCATTAACGGa                                                                  <  1:875206/147‑1 (MQ=255)
                                                                                    tggATTGGCGGTTGCTGAGCACTCTGAATCTGTGGCTGCGGCTCCGGCCGATGGTGCCGTTGCCGCTCTGTAGACGTCGTCCCCATCTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGa                                                                  <  1:1144391/149‑1 (MQ=255)
                                                                                           ggggTTGTTGAGTCGCCGGACTTTGCGGCTGCTGCACCGGCTGAATTTGAGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGTCATTAACGGAg                                                                 <  2:1114817/141‑1 (MQ=255)
                                                                                            ccgtggttgAGTCGGCTGAGGTTGTCGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCGTTTTCGGCGGCGGCGTAATCAACATGTCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGc                                                                <  2:489524/138‑1 (MQ=255)
                                                                                            cGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGAccg                                                          <  1:1029799/149‑1 (MQ=255)
                                                                                            cGGTGGTGGAGTGGCCTGAATGTGTGGCTGGTGCACCGGCTGAGTTTGCGGTTGCTACGCTGTAGACGTCGTGCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGc                                                                <  2:66240/143‑1 (MQ=255)
                                                                                                      gTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTgg                                                <  1:1300275/149‑1 (MQ=255)
                                                                                                      gTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCCCTGTAGACGTCGTCCCCATTTTCGCCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTTAccggtgc                                                      <  2:202299/143‑1 (MQ=255)
                                                                                                       tAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGa                                               >  1:807162/1‑149 (MQ=255)
                                                                                                          ccTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGCAGCTGACCGGTGCCGGTGGAttt                                            <  1:972702/149‑1 (MQ=255)
                                                                                                            tGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTgg                                                >  2:969345/1‑143 (MQ=255)
                                                                                                             gAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGaa                                         <  1:492521/149‑1 (MQ=255)
                                                                                                              aaTTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGTATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAg                                        <  1:1076339/149‑1 (MQ=255)
                                                                                                               aTTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGAtt                                             <  2:955446/143‑1 (MQ=255)
                                                                                                                tttcggggtgctccACCGGCTGAATTTGCGGTGGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGAttt                                            <  2:732688/135‑1 (MQ=255)
                                                                                                                 ttGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAgtgt                                     <  1:1221339/149‑1 (MQ=255)
                                                                                                                  tgtgGCTGCTGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTgc                                          <  2:831749/143‑2 (MQ=255)
                                                                                                                      gctgctGCACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGaa                                <  1:909352/149‑1 (MQ=255)
                                                                                                                      gctgctGCACCGGCTGAATTTGCGAATGCTGCGCAGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGaa                                >  1:655852/1‑149 (MQ=255)
                                                                                                                       ctgctgCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAAc                               >  1:143617/1‑149 (MQ=255)
                                                                                                                        tgctgcGCCGGCTGAAGTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAAGTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGGAGTGTc                                    <  2:1120176/143‑1 (MQ=255)
                                                                                                                        tgctgcACCGGCTGAATTTGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTc                                    >  2:552405/1‑143 (MQ=255)
                                                                                                                         gctgcACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCAATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGGGGATTTGAAGTGGCa                                   >  2:745063/1‑143 (MQ=255)
                                                                                                                            gcACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGaa                                >  2:496648/1‑143 (MQ=255)
                                                                                                                               ccGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAACTGACAGGTGCCGGTGGATTTGAAGTGGCAGAACAGCCTgcc                       >  1:919145/1‑149 (MQ=255)
                                                                                                                                  gCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTgccagc                    <  1:1181083/149‑1 (MQ=255)
                                                                                                                                         ttGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTgccagcc                   >  2:197532/1‑143 (MQ=255)
                                                                                                                                         ttGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGAGGATTTCGAGTCACAAAACAGCGTggccgcc                   >  2:853050/1‑143 (MQ=255)
                                                                                                                                         ttGCGGTTGCTACGCGGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATGAGTATTTGCAGGCGCAGTGCCATTAGCGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTGAGAACAGCCTgccagcc                   >  2:947302/1‑143 (MQ=255)
                                                                                                                                          tGCGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCAGTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTgccagcca                  <  2:414840/143‑1 (MQ=255)
                                                                                                                                           gctgTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGaa           <  1:280111/146‑1 (MQ=255)
                                                                                                                                            cGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATa                >  2:15563/1‑143 (MQ=255)
                                                                                                                                            cGGTTGCTACGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAGGTGTCAGAACAGCATGCCAGCCATa                >  2:532782/1‑143 (MQ=255)
                                                                                                                                               ttGCTGCGCTGTAGACGTCGTCCCCGTTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATGTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCa       <  1:489368/149‑1 (MQ=255)
                                                                                                                                               ttGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTACCGTAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCa       <  1:1183148/149‑1 (MQ=255)
                                                                                                                                                  ctgcGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGaaa          <  2:698245/143‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCGGAATGTTCCCATACTGACGGTTATAGACGATGCGTCCGTTTTCCATCTGTACCGGCTGCTGAGCTACTGGCTGCACTGGCTGGATTTGCGGTTGTTGAGTAGCCTGAATTTGTGGCTGCTGCACCGGCTGAATTTGCGGTTGCTGCGCTGTAGACGTCGTCCCCATTTTCGGCGGCGGCGTAATCAACATACCAGAATTAGTATTTGCAGGCGCATTGCCATTAACGGAGCTGACCGGTGCCGGTGGATTTGAAGTGTCAGAACAGCCTGCCAGCCATAGCGAAACCAGTGAC  >  CP009273/2861769‑2862065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: