Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,964,505 T→C 6.6% intergenic (+13/‑125) ygdQ → / → ygdR UPF0053 family inner membrane protein/DUF903 family verified lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,964,5050TC 6.6% 187.8 / 2.6 75intergenic (+13/‑125)ygdQ/ygdRUPF0053 family inner membrane protein/DUF903 family verified lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (31/37);  new base C (3/3);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

ATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCA  >  CP009273/2964358‑2964644
                                                                                                                                                   |                                                                                                                                           
aTTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCCACAAAAAGAATCCGCTCTGATACTCCGTACGctc                                                                                                                                            >  1:527216/1‑149 (MQ=255)
 ttCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGctct                                                                                                                                           >  1:29946/1‑149 (MQ=255)
  tCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCtctc                                                                                                                                          <  1:193913/149‑1 (MQ=255)
      gtggGCTGTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGctc                                                                                                                                            <  2:1133821/143‑1 (MQ=255)
             ttctcgtgcttCGGGATAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAACTCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCg                                                                                                                                     <  2:644899/134‑1 (MQ=255)
              tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                                                              >  1:1023209/1‑149 (MQ=255)
              tACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGc                                                                                                                              >  1:935861/1‑149 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTa                                                                                                                           >  1:801482/1‑149 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCTGCGGGAGGGCGTa                                                                                                                           >  1:216013/1‑149 (MQ=255)
                 ccTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTa                                                                                                                           <  1:863848/149‑1 (MQ=255)
                  cTGCTTCTGGAAGGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTGCTTCTCTATTGCTGGTGAAAGCCTCAACGTGATTCGCAGCAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCgggggg                                                                                                                                >  2:391649/1‑143 (MQ=255)
                  cTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTCATTCGCGGTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAAGCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAgg                                                                                                                                >  2:561016/1‑143 (MQ=255)
                   ctaTTCTGGAGAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTCCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTact                                                                                                                         <  1:472067/147‑1 (MQ=255)
                     aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATTTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                             <  2:149899/143‑1 (MQ=255)
                     aTTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCg                                                                                                                             <  2:385781/143‑1 (MQ=255)
                     aTTCTGGAAAGTTCCGACATCCACGTACCGAAAGGTTACATCTACTGCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactca                                                                                                                       <  1:1169135/149‑1 (MQ=255)
                      ttCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGCGGGCGt                                                                                                                            >  2:182608/1‑143 (MQ=255)
                      ttCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac                                                                                                                      >  1:557103/1‑149 (MQ=255)
                         cggAAAGTCTCGACATCCACCGACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTAGTGCGGTTGAAAGCCTCCACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCCCCTGCATGATGTCGTact                                                                                                                         <  2:694152/142‑1 (MQ=255)
                           gAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCtt                                                                                                                 >  1:178548/1‑149 (MQ=255)
                             aaGTTTCGACATCCACGTACCGAAAGGTTACATCTATTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACATGATTCGCAACAAAAATAATCCGCTCTGATACTCCGTACGCCCTCCCGCATGAGAGCGTacggagtatcaga                                                                                                               <  1:920022/149‑12 (MQ=255)
                               gTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTcc                                                                                                                   <  2:26698/143‑1 (MQ=255)
                                tttCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACCAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCt                                                                                                                  >  2:987849/1‑143 (MQ=255)
                                  tCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCt                                                                                                          <  1:48882/149‑1 (MQ=255)
                                    gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                        >  1:1173654/1‑149 (MQ=255)
                                    gACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTca                                                                                                        >  1:1130133/1‑149 (MQ=255)
                                     aCATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTAc                                                                                                             <  2:1035930/143‑1 (MQ=255)
                                            cGTACCGAACGGTTACATCTACTGCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTaa                                                                                                <  1:197791/149‑1 (MQ=255)
                                              tACCGGAAGGTTACATGTACTTCGCGATGTTTTTCTCTATTGCGGGTGAAAGCGTCAACTTGAGTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTACTTTGTCCCTCACAGt                                                                                                    <  2:20496/143‑1 (MQ=255)
                                                ccGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtt                                                                                            <  1:775651/149‑1 (MQ=255)
                                                   aaaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                         <  1:472180/149‑1 (MQ=255)
                                                   aaaGGTTACATCTACTTCGCGATGTTCTTCTCGATGGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTc                                                                                         <  1:1121113/149‑1 (MQ=255)
                                                      ggTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCCCCCCTTTTACCCTCACAGTTTAAGAtt                                                                                            >  2:150222/1‑143 (MQ=255)
                                                       gTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGc                                                                                     <  1:1038007/149‑1 (MQ=255)
                                                        ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGACTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCATACTCACTCCTTTTACCCTCAACGATTAAGAtttt                                                                                          >  2:86530/1‑143 (MQ=255)
                                                          aCATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAGAAAGAATCCGCTCTGATACTCCGTCCGCTCTCCTGCGGGAGGGCGTACTCTCTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCttt                                                                                  <  1:844227/149‑1 (MQ=255)
                                                               tACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGa                                                                             <  1:39569/149‑1 (MQ=255)
                                                                aCTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACACTTTAAGATTTTCCTGCTTTCAAGat                                                                            <  1:775984/149‑1 (MQ=255)
                                                                     gcgATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATATTCCTGCTTTCAGGa                                                                             >  2:454592/1‑143 (MQ=255)
                                                                       gaggtgcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCCCAAAGTTTGAGATTTTCCTGCTTTCAAGATATATAAc                                                                     <  1:637495/143‑1 (MQ=255)
                                                                        aTGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGatat                                                                          <  2:846331/143‑1 (MQ=255)
                                                                             cttctCTGTTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTTCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAAc                                                                     <  2:821916/143‑1 (MQ=255)
                                                                               tctcTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTTTGATGCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCCAGATATATAACGTCGGttt                                                             <  1:946165/149‑1 (MQ=255)
                                                                                   tATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                               >  2:959506/1‑143 (MQ=255)
                                                                                   caTTGCGGTTGAACGCCTCAACGTGATTCGCAACAAACAGAATCCGCTCCGATCCTCCGTCCGCTCTCCTGCGCGAGGGCGTACTCACTCCTTTTTCCCTCACCGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt                                                               <  2:550458/142‑1 (MQ=255)
                                                                                    aTTGCGGTTGAAAGCCTCAACTTGAGTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACACTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTAACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGtt                                                              <  2:311472/143‑1 (MQ=255)
                                                                                     ttGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGttt                                                             <  2:1116571/143‑1 (MQ=255)
                                                                                     tggCGGGTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGCTTAAGATTTTCCTGCTTTCAAGATATATACCGTCGGttt                                                             >  2:661024/3‑143 (MQ=255)
                                                                                      tGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATCCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAGGATATATAACGTCGGTTta                                                            <  2:1106615/143‑1 (MQ=255)
                                                                                        cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTtata                                                          >  2:765685/1‑143 (MQ=255)
                                                                                        cGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGa                                                    >  1:77917/1‑149 (MQ=255)
                                                                                          gTTGAAATCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCGCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGCTATATAACGTCGGTTTATaaa                                                        <  2:958806/143‑1 (MQ=255)
                                                                                          gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATaaa                                                        >  2:1084070/1‑143 (MQ=255)
                                                                                               acgCCTCAACTTGATTCGCAAAAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATAGAACGTCGGTTTATAAACAGAc                                                   <  2:1098020/141‑1 (MQ=255)
                                                                                                  ccTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACCGACTATTATCAt                                          >  1:876329/1‑149 (MQ=255)
                                                                                                   cTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta                                         <  1:1066000/149‑1 (MQ=255)
                                                                                                      aaCTTGATTCGCAACAAAAAGAATCCGCTCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATc                                            <  2:936672/143‑1 (MQ=255)
                                                                                                      aaCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggt                                      <  1:1226223/149‑1 (MQ=255)
                                                                                                        cTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag                                    >  1:436676/1‑149 (MQ=255)
                                                                                                         ttGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta                                         >  2:668686/1‑143 (MQ=255)
                                                                                                              tCGCAACAAGAAGAATCCGCTCTGATACTCCGTACGCTCTCCTACGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAtaggtag                                    >  2:587985/1‑143 (MQ=255)
                                                                                                              tCGCAACAAAAAGACTCCGCTCTGATACTCCGTTCGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATTTTCCTGCTCTCAAGATATATAACGTCGGTTTAGAAACAGACTATTATCATAGGTAGGCCaaa                              <  1:792454/149‑1 (MQ=255)
                                                                                                               cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTCAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAt                             <  1:1218960/149‑1 (MQ=255)
                                                                                                               cGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTCTATAAACAGACTATTATCATAGGTAGGCCAAAt                             <  1:951956/149‑1 (MQ=255)
                                                                                                               cGCAACAAAAAGAATCCGCTCTGATACTCCATACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAAt                             >  1:673995/1‑149 (MQ=255)
                                                                                                                 caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGa                           >  1:1234334/1‑149 (MQ=255)
                                                                                                                  aacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACAATTATCATAGGTAGGCCAAATGAg                          <  1:969812/149‑1 (MQ=255)
                                                                                                                      aaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATa                      >  1:1189724/1‑149 (MQ=255)
                                                                                                                       aaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGa                           <  2:761750/143‑1 (MQ=255)
                                                                                                                             tCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAGCAGACTATTATCATAGGTAGGCCAAATGAGGATaa                     >  2:1011988/1‑143 (MQ=255)
                                                                                                                                  tctGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa          >  1:998143/1‑149 (MQ=255)
                                                                                                                                        cgtCCGTACGCCCTCCTGAGGGAGGTCGTACCCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTGCAAGATATATAACGTCGGTTTATAAACAGACCATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaa          <  2:358886/141‑1 (MQ=255)
                                                                                                                                        aCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg    >  1:344874/1‑149 (MQ=255)
                                                                                                                                          tCCGTACGCTCTCATGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAACCAGACTATTATCATAGGTAGGTCAAATGAGGATAACCGGATGAAAAAATGGGCa  >  1:888574/1‑149 (MQ=255)
                                                                                                                                           ccGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAAt       <  2:1091094/143‑1 (MQ=255)
                                                                                                                                            cGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATGGGTAGGCCAAATGAGGATAACCGGATGAAAAAATg      >  2:167196/1‑143 (MQ=255)
                                                                                                                                              tACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg    <  2:224522/143‑1 (MQ=255)
                                                                                                                                              tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg    >  2:874814/1‑143 (MQ=255)
                                                                                                                                              tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACAGGATGAAAAAATggg    >  2:642262/1‑143 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
ATTCTGGTGGGCTTTACCCTGATTCTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCA  >  CP009273/2964358‑2964644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑