Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,390,205 G→T 52.5% G34V (GGG→GTG)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,390,2050GT52.5% ‑1.8 / 90.4 77G34V (GGG→GTG) hfqglobal sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base G (12/24);  new base T (14/26);  total (26/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

ATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTA  >  CP009273/4390060‑4390352
                                                                                                                                                 |                                                                                                                                                   
aTCGAACGGTCCAAAGTACAAAGAAGCATATAAGGAACAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCa                                                                                                                                                  <  1:15330/149‑1 (MQ=255)
aTCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCa                                                                                                                                                  <  1:379017/149‑1 (MQ=255)
   gAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAAt                                                                                                                                               <  1:1277928/149‑1 (MQ=255)
      aGGTTTAAAGTCAAAAATTGCATATAAGGGAAAGAGCGCATGGATAGGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGa                                                                                                                                            <  1:594173/149‑1 (MQ=255)
       ggTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAg                                                                                                                                           <  1:1195706/149‑1 (MQ=255)
         ttCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAACTCGAGTc                                                                                                                                         >  1:40964/1‑149 (MQ=255)
         ttCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTAGAGTTTCTCTTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAAt                                                                                                                                               >  2:259200/1‑143 (MQ=255)
                tACAACTCAGCAGATAAGGAAAAGAGATAATGGCCAAGGGGCAATCTTTACACGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTACGCTGCAAGGGCAAATCGAGTCTTTTGAt                                                                                                                                  <  1:317439/149‑1 (MQ=255)
                tACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGAt                                                                                                                                  <  1:984623/149‑1 (MQ=255)
                    aaTAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTATTTTg                                                                                                                                    >  2:424329/1‑143 (MQ=255)
                      tAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGAt                                                                                                                                  <  2:90761/143‑1 (MQ=255)
                          cATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGa                                                                                                                        >  1:1250562/1‑149 (MQ=255)
                              taAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGt                                                                                                                          >  2:1037061/1‑143 (MQ=255)
                                aGGAGACGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGAACTGCGTCGGGGACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCGAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGt                                                                                                                  >  1:382208/1‑149 (MQ=255)
                                aGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGa                                                                                                                        <  2:947319/143‑1 (MQ=255)
                                 ggAAAAGAGAGAATGGCTAAGGGCCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                                                                                 <  1:833926/149‑1 (MQ=255)
                                 ggAAAAGAGAGAATGGCGAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGAGTTCCAGTTTCTAGTTATTTGTTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGGTCGTGAATCTGtg                                                                                                                 >  1:711376/1‑148 (MQ=255)
                                       gggcagaTGGCTAAGGGGCAATCTTTACAAGGTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                                                                                 <  2:201532/137‑1 (MQ=255)
                                       gatggaATGGCTGAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                                                                                 <  2:178809/139‑1 (MQ=255)
                                       gaggggaTGGCGAAGGGGCAAGCTTTACAAGGTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTCAATGGTCTTAAGCGGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGtt                                                                                                                 <  2:735407/137‑1 (MQ=255)
                                          agaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGaa                                                                                                              <  2:1063657/143‑1 (MQ=255)
                                          agaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAacac                                                                                                        <  1:428465/149‑1 (MQ=255)
                                           ggacggCTAAGGGGCAACCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                                                       <  1:717743/145‑1 (MQ=255)
                                           gaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                                                       <  1:139082/149‑1 (MQ=255)
                                           gaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                                                       <  1:987677/149‑1 (MQ=255)
                                           gaATGGCCAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                                                       <  1:544977/149‑1 (MQ=255)
                                            aaTGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACgg                                                                                                      <  1:76371/149‑1 (MQ=255)
                                            aaTGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTATATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTTTTGAAAAACACgg                                                                                                      >  1:326705/1‑149 (MQ=255)
                                             aTGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGt                                                                                                     <  1:1219427/149‑1 (MQ=255)
                                               ggCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAaca                                                                                                         <  2:52494/143‑1 (MQ=255)
                                                  tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCGTGTTGCAAAGCACGGTCAGcc                                                                                                >  1:149715/1‑149 (MQ=255)
                                                   aaGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTTGTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGt                                                                                                     >  2:744739/1‑143 (MQ=255)
                                                   aaGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGt                                                                                                     >  2:664857/1‑143 (MQ=255)
                                                   aaGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGCGATCCTGTTGAAAAACACGGt                                                                                                     >  2:309213/1‑143 (MQ=255)
                                                     ggggCAATCTTTACAAGATCCGTTCCGGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGa                                                                                             <  1:1175115/149‑1 (MQ=255)
                                                        gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAAGCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATgg                                                                                          >  1:430916/1‑149 (MQ=255)
                                                        gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATGTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATAGAGTCTTTTGATATGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                                                                                >  2:1099137/1‑143 (MQ=255)
                                                          aaTCTTAACAAGATCCGTTCATGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCCAATCCAGTTTTTTGATCAGTTCGTGATCCTGGTGAAAAACACGGTCAGCCAGATGGtt                                                                                        <  1:1224868/149‑1 (MQ=255)
                                                           aTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGGAAAACACGGTCCGCCAGa                                                                                             >  2:945962/1‑143 (MQ=255)
                                                           aTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAAAACGATGAGCCaaa                                                                                             >  2:143829/1‑141 (MQ=255)
                                                            tCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTTTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGAt                                                                                            <  2:594909/143‑1 (MQ=255)
                                                             cTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATACTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                                                                                     >  1:563537/1‑149 (MQ=255)
                                                                 aCAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAAACTGTTGGGCGGTTTTTGATCCTTTTGAAAACCACGGTCAGCCAGATGGttt                                                                                       >  2:511728/1‑143 (MQ=255)
                                                                 aCAAGATCCGTTCCTGAACGCACGGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGc                                                                                 <  1:280863/149‑1 (MQ=255)
                                                                  cAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTGATTTGGTGAATGGTATTAAGCTGCAAGTGCAAACCGAGTCTTTTGAGCAGATCGTGATCCTGTTGAAAAACACGGTCAGCCAGAAAGtttg                                                                                      >  2:35556/1‑142 (MQ=255)
                                                                    aGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                                                                              <  1:774117/149‑1 (MQ=255)
                                                                    aGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                                                                              <  1:1240576/149‑1 (MQ=255)
                                                                    aGACCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACa                                                                                    <  2:361229/143‑1 (MQ=255)
                                                                     gATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACaa                                                                                   >  2:704142/1‑143 (MQ=255)
                                                                      aTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgcg                                                                            >  1:939276/1‑149 (MQ=255)
                                                                        ccGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGTCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAt                                                                          <  1:1117540/149‑1 (MQ=255)
                                                                          ggtCCTGAACGCAGTGCGTCGCGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGCATTAAGCCGCGAGAGCCTTGCGGTTCCCGGTTTCAGTTTGTAAAAAAAAAAAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                                                                              <  2:1102964/141‑1 (MQ=255)
                                                                          gTTCCTGAACGCGGCGCGGCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                                                                              <  2:107672/143‑1 (MQ=255)
                                                                          gTTCCTGAACGAACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcg                                                                              <  2:697069/143‑1 (MQ=255)
                                                                               aagacagcatGCGTCGGGAACGTGTGCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGGGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACt                                                                   <  1:770296/140‑1 (MQ=255)
                                                                                    gCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAACTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACACGATTTCTACtgttgt                                                              >  1:1015365/1‑149 (MQ=255)
                                                                                             cGGGAACGTGTTCCTGTTTCTAGTTATTTGGTTAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTCTTCAAAACCACGGTTAGCCAGATGGTTTACAAGCACGCGACTTCTACTGTTGTccc                                                           <  2:623404/143‑1 (MQ=255)
                                                                                              gggAACGTGTTCCAGGTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCg                                                          <  2:581248/143‑1 (MQ=255)
                                                                                                      gtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGACTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtc                                            <  1:493512/149‑1 (MQ=255)
                                                                                                            gcttCTAGTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAACCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAATACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCGACTGTTGTCCCGTCTCGCCCGGTTTCtcatca                                      <  1:289427/147‑1 (MQ=255)
                                                                                                                 tatgtattTGGCGAATGGTATTAAGCTGCAACTGCAAATCGAGTCTTTTGCTCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatc                                       <  2:226702/143‑1 (MQ=255)
                                                                                                                  attgagttGGTGAGTGGTGTTAAGCTGCAAGGGCAAATCGAGTCTTTTGCTCAGTTGGGGTTCTTGTGGAGAGACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatca                                      <  2:680632/137‑1 (MQ=255)
                                                                                                                    ttattgGGTGAATGGTATTAAGCGGCAAGGGCAAATCGGGTCTTTTGATCAGTTCGGGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATcaca                                    <  2:638038/143‑1 (MQ=255)
                                                                                                                      atttGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTaacaac                            >  1:168680/1‑149 (MQ=255)
                                                                                                                       tttGGTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTTTTGTCCCGTCTCGCCCGGTTTCTCATCACATTa                                 >  2:668089/1‑143 (MQ=255)
                                                                                                                          ggTGAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCg                        <  1:776918/149‑1 (MQ=255)
                                                                                                                             gAATGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTg                     >  1:643422/1‑149 (MQ=255)
                                                                                                                              aaTGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTgg                    <  1:1096601/149‑1 (MQ=255)
                                                                                                                                tGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                  <  1:384674/149‑1 (MQ=255)
                                                                                                                                tGGTATTAAGCTGCAAGTGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACAAGGTAAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTTTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCg                        >  2:1041423/1‑143 (MQ=255)
                                                                                                                                gggTAGTAAGCTGCAAGTGCAACTCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                  <  1:245199/148‑1 (MQ=255)
                                                                                                                                  gTATTAAGCTGCAAGGGCAAATCGAGGCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGt                <  1:1096149/149‑1 (MQ=255)
                                                                                                                                     ttagtgtGCAAGTGCGAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGc                   <  2:230096/137‑1 (MQ=255)
                                                                                                                                      tAAGCTGCAAGGTCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTGGTCCCGTCTCGCCCGGTTTCTCATCACAGTCACAACGCCGGTGGCg                  <  2:623478/143‑1 (MQ=255)
                                                                                                                                      tAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                  <  2:99470/143‑1 (MQ=255)
                                                                                                                                           tGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTAcc             <  2:539159/143‑1 (MQ=255)
                                                                                                                                               aGGGCAAATCGAGTTTTTGGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACcagca         <  2:507538/143‑1 (MQ=255)
                                                                                                                                                gggCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAAGCGATTTCTAATGTTGTCCCGTCTCGACCGGTTTCTCATCACCGTAACAACGCCGGTGGGGGTCCAAGCCGTGACTa  >  1:268149/1‑149 (MQ=255)
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ATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTA  >  CP009273/4390060‑4390352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: