Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 454,949 G→T 26.8% S202S (TCG→TCT lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273454,9490GT26.8% 127.0 / 39.9 73S202S (TCG→TCTlonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (29/24);  new base T (6/14);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 7.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGCATCGACGATCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAA  >  CP009273/454814‑455094
                                                                                                                                       |                                                                                                                                                 
tAGCATCGACGATCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCg                                                                                                                                            >  2:669705/1‑143 (MQ=255)
   cATCGACGATCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATc                                                                                                                                         >  2:965157/1‑143 (MQ=255)
    ctCGACGATCCGGCGCGTCTGGCGGATGCCATTGCTTCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCAAAGTTATCGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCa                                                                                                                                  <  1:576756/148‑1 (MQ=255)
          gaTCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTga                                                                                                                            >  1:47504/1‑149 (MQ=255)
               ggCGCGTCTGGCCGATACCATTGCTGCCCATATGCCGCTGAACCTGGCTGACCAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTg                                                                                                                             <  2:914387/143‑1 (MQ=255)
                   cgTCTGGCGGATACCATTGCTGCACATCTGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGGCTGGAATATCTGATGGCAGTGATTGAATCGGGACTCGAACTGCTGCAGGTGGAGaa                                                                                                                         >  2:212868/1‑143 (MQ=255)
                     tCTGGCGGACACCCTTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAAc                                                                                                                       <  2:64916/143‑1 (MQ=255)
                                 cATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAAcc                                                                                                           >  2:56098/1‑143 (MQ=255)
                                 cATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTa                                                                                                     >  1:467260/1‑149 (MQ=255)
                                 cATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAAACGATCTGCTGCAGGTTGAGAAACGCATTAGCAAcc                                                                                                           >  2:186483/1‑143 (MQ=255)
                                 cATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAACCTGAAATCGACCTGCTGCAGGCTGAGAAACGCATTCGCAAcc                                                                                                           >  2:261931/1‑143 (MQ=255)
                                 cATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGAAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAAcc                                                                                                           >  2:519681/1‑143 (MQ=255)
                                 cATTGCTGCACATATGCCGCTGAAACTGACTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGGTa                                                                                                     >  1:695754/1‑149 (MQ=255)
                                     gctgcACCTATGCCGCGGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTaaaaa                                                                                                 <  1:775521/149‑1 (MQ=255)
                                      ctgcACATATGCAGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGGGAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGtt                                                                                                      >  2:837592/1‑143 (MQ=255)
                                       tgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGc                                                                                               <  1:700974/149‑1 (MQ=255)
                                        gcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCGACCGCGTTaa                                                                                                    >  2:466939/1‑143 (MQ=255)
                                         cacaTATGCCGCTGAAACTGGCTGACAAACAGTCTCTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTaaa                                                                                                   >  2:702971/1‑143 (MQ=255)
                                            atatGCCGGCGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATg                                                                                          <  1:221914/149‑1 (MQ=255)
                                            atatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCCGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGCTg                                                                                          >  1:1246313/1‑149 (MQ=255)
                                            atatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAg                                                                                                <  2:1128160/143‑1 (MQ=255)
                                            atatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATg                                                                                          <  1:528223/149‑1 (MQ=255)
                                             tgtGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGc                                                                                               <  2:285225/141‑1 (MQ=255)
                                             tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGc                                                                                               <  2:1120560/143‑1 (MQ=255)
                                             gagtccGCTGAAACGGGCGGCCAAACGGTCTGTTCGGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGGGAAACGCATTCGCAACCGCGTTAAAAAGc                                                                                               <  2:49345/139‑1 (MQ=255)
                                              atGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGga                                                                                        >  1:110793/1‑149 (MQ=255)
                                                gCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGgaga                                                                                      <  1:404962/149‑1 (MQ=255)
                                                   gCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAAt                                                                                   <  1:158394/149‑1 (MQ=255)
                                                           tGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGt                                                                           <  1:709231/149‑1 (MQ=255)
                                                           tGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGACGGAGAAATcc                                                                                 <  2:543624/143‑1 (MQ=255)
                                                           tGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGAACCCGGGGGCAAACGCAGACGAAGaaagtcttgcgt                                                                           >  1:1267922/1‑140 (MQ=255)
                                                             gCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCa                                                                               >  2:191888/1‑143 (MQ=255)
                                                               tgccAAACAGTCTGTTCTGGAGATGTCCCACCTTCCGGAACGGCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGt                                                                       <  1:357993/146‑1 (MQ=255)
                                                                   aaaCAGTCTGTTCTGGAGGTGGCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGa                                                                         <  2:421344/143‑1 (MQ=255)
                                                                      cAGGCTGTTCTGGAGATGCCCGACGTTAACGAACGTCTGGAATATCTGCAGGCATTAATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCt                                                                <  1:37633/149‑1 (MQ=255)
                                                                         tCTGTTCTGGAGATGTCCGACGTTAACGGACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCATATGGAGAAATCCCAGGGTggcgactc                                                                   >  2:629180/1‑136 (MQ=255)
                                                                         tCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGaa                                                             <  1:430100/149‑1 (MQ=255)
                                                                         tCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTa                                                                   >  2:316224/1‑143 (MQ=255)
                                                                          cTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTAt                                                                  <  2:745387/143‑1 (MQ=255)
                                                                            gTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATCACTGATTGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGa                                                          <  1:884755/149‑1 (MQ=255)
                                                                             ttCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTg                                                               <  2:693137/143‑1 (MQ=255)
                                                                              tCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGc                                                        <  1:250354/149‑1 (MQ=255)
                                                                                  gagaTGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCACCCGTGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAAt                                                    >  1:570431/1‑149 (MQ=255)
                                                                                    gaTGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGc                                                        <  2:761393/143‑1 (MQ=255)
                                                                                    gaTGGCCGACGTTAACGAACGTCGGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGc                                                        <  2:900781/143‑1 (MQ=255)
                                                                                       gtgcGACGCTAACGCACGTCTGGAATATCTGCTGGCAATGATGGAATCGGAAATCGATCGGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAg                                               <  1:438422/146‑1 (MQ=255)
                                                                                        tCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGc                                              >  1:18137/1‑149 (MQ=255)
                                                                                        tCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGc                                              >  1:1153302/1‑149 (MQ=255)
                                                                                          cGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTa                                            >  1:1037978/1‑149 (MQ=255)
                                                                                           gACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCACGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTAt                                           <  1:871522/149‑1 (MQ=255)
                                                                                               ttAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCt                                             >  2:1067598/1‑143 (MQ=255)
                                                                                                 aacgaacgTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGAAAACGAAAGCTAt                                           >  2:551265/1‑143 (MQ=255)
                                                                                                  acgaacgTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCagaaa                                    <  1:378154/149‑1 (MQ=255)
                                                                                                  acgaacgTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCagaaa                                    <  1:409094/149‑1 (MQ=255)
                                                                                                  acaaacgTTTGGAAATTCTGAGGGAAATGATGGAATTTGAAATCGATCTGCTGCGGGCTGGGAACCGCATCCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTAtt                                          <  2:822069/140‑1 (MQ=255)
                                                                                                    gaacgTCTGGAATATCTGATGGCAATGATGGAATCGGCAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGAAAATGAAAGCTATTCggaaaga                                  >  1:260413/1‑149 (MQ=255)
                                                                                                      acgTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCaga                                      <  2:1122503/143‑1 (MQ=255)
                                                                                                       cgTCTGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCACAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCagaa                                     <  2:764478/143‑1 (MQ=255)
                                                                                                        gTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCagaaa                                    >  2:126325/1‑143 (MQ=255)
                                                                                                           tGGAATATCTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGt                           <  1:1097043/149‑1 (MQ=255)
                                                                                                           tGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGACCGAGCAAATGAACGCTATTCAGAAAGAACTCGGt                           >  1:616518/1‑149 (MQ=255)
                                                                                                            ggAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAAc                                >  2:1031494/1‑143 (MQ=255)
                                                                                                             gAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGa                         >  1:543831/1‑149 (MQ=255)
                                                                                                                tatCAGATGGCAATGATGGAATCTGAAATCCACCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAAt                      <  1:1006366/149‑1 (MQ=255)
                                                                                                                  tCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTg                          >  2:604169/1‑143 (MQ=255)
                                                                                                                   cTGATGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAAGTCGGTGAAATGga                   >  1:321857/1‑149 (MQ=255)
                                                                                                                      aTGGCAATGATGGACTCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAAt                      <  2:393021/143‑1 (MQ=255)
                                                                                                                      aTGGCAATGATGGAATCTGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAAt                      <  2:19163/143‑1 (MQ=255)
                                                                                                                         gCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATACCAGCGTGAGTACTATCTGAACGAGCACATGAAAGCTATTCAGGAAGAACTAGGTGAAATGGACGAcgc             >  1:977290/1‑149 (MQ=255)
                                                                                                                         gCAATGATGGAATCGGAAATCGATATGCTGGTTGTGTAGAAACGCATTCGCAACCGCGTTGAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGga                   >  2:807837/1‑143 (MQ=255)
                                                                                                                               atgGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGa       <  1:66072/149‑1 (MQ=255)
                                                                                                                                    aTCGGAAATCGATCTGCTGCAGGTTGAGAAACGGATTCGCAACCGCGTTAAAAAGCAGATCGCATTATCCCGGCCTGATCACTAGCTGAACGAGCAAATGAAAGCTATGCAGTAAGAACTCGGTGAAATGGACGACGCGCCGCACGaaa  >  1:953207/1‑149 (MQ=255)
                                                                                                                                     tCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCCGAAGGACAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGa       <  2:402316/143‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                                 
TAGCATCGACGATCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGATCTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAA  >  CP009273/454814‑455094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: