Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 468,288 A→T K79* (AAG→TAG)  glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273468,2880AT98.9% 292.2 / NA 94K79* (AAG→TAG) glnKnitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (46/47);  minor base G (1/0);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAG  >  CP009273/468142‑468431
                                                                                                                                                  |                                                                                                                                               
tCACCGAAGTGAAAGGTTTCCGGCCTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATCACCAACTCGATGAAGTGATCGATATCGTCAGTTAg                                                                                                                                               <  2:1175057/149‑1 (MQ=255)
     gAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAggcggc                                                                                                                                          <  2:1533626/149‑1 (MQ=255)
        gTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGAGACAAAGTCTTCCTCACTTCCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCTGTTAGGCGGCTTa                                                                                                                                       <  2:1216727/149‑1 (MQ=255)
         tGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGAGGCGATTGCTGATGACCAACTCGATGAAGTGATCGAGAACGACAGTTAggcggcg                                                                                                                                         >  1:341096/1‑145 (MQ=255)
          gAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTcca                                                                                                                                     >  2:79679/1‑149 (MQ=255)
           aaaGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTa                                                                                                                                       >  1:72731/1‑146 (MQ=255)
             aGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCg                                                                                                                                  >  2:539934/1‑149 (MQ=255)
              ggTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCgg                                                                                                                                 <  2:906638/149‑1 (MQ=255)
               gTTTCGGGCGTCAGAAGGGGCATGCCGGGCGGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGGGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGa                                                                                                                                <  2:1659773/149‑1 (MQ=255)
               gTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCTGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATTTCGTCAGTTAGGCGGCTTACAcc                                                                                                                                   <  1:833034/146‑1 (MQ=255)
                     ggCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGAAAAAATTGATGTGGCGATTGCTGATGGCTAAAGGTTTGTAGGGAGCGAGATCGGCGGTGAGGCGGCTTACACCGGAAAAAtt                                                                                                                          >  2:499219/1‑149 (MQ=255)
                     ggCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTGCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAAtt                                                                                                                          <  2:80087/149‑1 (MQ=255)
                          cAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTgg                                                                                                                        >  1:1172549/1‑146 (MQ=255)
                           aGAAAGGGCATGCCGAGCCGTACCGGGGGGCGGGATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGc                                                                                                                       <  1:484756/146‑1 (MQ=255)
                            gAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACg                                                                                                                   >  2:680340/1‑149 (MQ=255)
                             aaaGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGAGGCTTACACCGGAAAAATTGGCGa                                                                                                                     >  1:1260991/1‑146 (MQ=255)
                             aaaGGGCAGGCGGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACgg                                                                                                                  <  2:623508/149‑1 (MQ=255)
                                   cATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAAt                                                                                                            >  2:1764302/1‑149 (MQ=255)
                                     tGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGCTATCGTCAGTTAGGCGGCTTCCACCGGAACACTTGGCGACGGCAACATCt                                                                                                          >  2:556162/1‑149 (MQ=255)
                                      gCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCtt                                                                                                         <  2:955815/149‑1 (MQ=255)
                                              gTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTtcgtcg                                                                                                    <  1:716928/146‑1 (MQ=255)
                                              gTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTtcgctt                                                                                                    >  1:1577723/1‑143 (MQ=255)
                                              gTACCAGGGGACGGAATACAGCGCCAATTACCTGCCAAAATTAAAAATTTATTTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTtcgtcg                                                                                                    >  1:957633/1‑146 (MQ=255)
                                               tACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGa                                                                                                >  2:1684253/1‑149 (MQ=255)
                                                aCCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCt                                                                                                  >  1:454996/1‑146 (MQ=255)
                                                   ggggggCGGAATCCAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTg                                                                                            <  2:1112871/149‑1 (MQ=255)
                                                       ggCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGc                                                                                           <  1:181184/146‑1 (MQ=255)
                                                            aaTACGGCGTTAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAAcgc                                                                                      <  1:1362626/146‑1 (MQ=255)
                                                             aTACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAAcgcg                                                                                     >  1:380159/1‑146 (MQ=255)
                                                                cAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattc                                                                               <  2:1709284/149‑1 (MQ=255)
                                                                      cAAGTTCCTGACAAAAGGAAAAATTGATGCGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgtattc                                                                         <  2:123051/149‑1 (MQ=255)
                                                                        aTTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgtattcgt                                                                       >  2:1493585/1‑149 (MQ=255)
                                                                        aTTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCattcgtattcgt                                                                       >  2:1493681/1‑149 (MQ=255)
                                                                           tCCTGCCCAACGGAAAAATTGATGTGGCGATTGCTGATGACCAACGCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAAGTGGCGACGACAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTAcc                                                                    <  2:600659/149‑1 (MQ=255)
                                                                            ccTGCCAAAAGTAAAGATTGATGTGGTGATTGCTGGTGACCAACGCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCAttcgtattcgta                                                                      <  1:1583770/146‑1 (MQ=255)
                                                                            ccTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGCTATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCg                                                                   >  2:697225/1‑149 (MQ=255)
                                                                              tGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGc                                                                 >  2:1135204/1‑149 (MQ=255)
                                                                              tGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGGATTCGTACCGGc                                                                 >  2:1434366/1‑149 (MQ=255)
                                                                              tGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGACAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGGATTGCAACGCGTCATTCGTATTCGTACCGGc                                                                 >  2:1192035/1‑149 (MQ=255)
                                                                              tGCCAAAAGTAAAAATTGATGTGGCGATTGCTGAGGACCAACTCGATGAAGTGATCGATATCGGCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGc                                                                 <  2:158135/149‑1 (MQ=255)
                                                                                ccAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGa                                                               >  2:1649694/1‑149 (MQ=255)
                                                                                ccAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGa                                                               >  2:1715734/1‑149 (MQ=255)
                                                                                ccAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTAAGTTAGGCGGCGTACACCGGAAAAATTGGCGACGGCAAAATCTTAGTAGCTGAATTGCAACGCGTCACGCGACTTAGTACCgg                                                                  >  1:1305135/1‑146 (MQ=255)
                                                                                  aaaaGCAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAg                                                             <  2:545412/149‑1 (MQ=255)
                                                                                    aaGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCGTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGaa                                                              >  1:271558/1‑146 (MQ=255)
                                                                                    aaGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCGTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGCAATTCGTATTCGTACCGGCGaa                                                              >  1:271521/1‑146 (MQ=255)
                                                                                     aGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCcg                                                          <  2:718427/149‑1 (MQ=255)
                                                                                          aaaTTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCcgac                                                        <  1:1224125/146‑1 (MQ=255)
                                                                                                  gtgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCt                                             >  2:624437/1‑149 (MQ=255)
                                                                                                  gtgGCGATTGCTGATGACCAACGCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCt                                             <  2:209735/149‑1 (MQ=255)
                                                                                                   tgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAgcggcg                                               >  1:192688/1‑146 (MQ=255)
                                                                                                   tgGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGAAGAAGCGGCGCTg                                            >  2:400442/1‑149 (MQ=255)
                                                                                                    ggCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGt                                           >  2:378960/1‑149 (MQ=255)
                                                                                                     gCGATTGCTGATTACCAACGCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATCCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCt                                             <  1:1016835/146‑1 (MQ=255)
                                                                                                     gCGATTGCGGGTGACCAACTGGATGAAGTGATTGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTAGTCGTACCGGCGAAGCCGACGAAGCGGCGCt                                             <  1:510411/146‑1 (MQ=255)
                                                                                                      cGATTGCTGATGACCAACTCGATGAAGTGATCGATCTCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTg                                            >  1:1021434/1‑146 (MQ=255)
                                                                                                       gaatacTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGt                                           >  1:1457633/6‑146 (MQ=255)
                                                                                                       gATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAGTTGCAACGCGTCATTCGTATTCGTACCGGCGACGCCGACGACGCGgcgcgggacg                                        >  2:197940/1‑143 (MQ=255)
                                                                                                         ttggtcatgaCCAACTCGATGAAGTGCTTGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAAGTGCAACGCGTGATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAAtct                                      <  2:254672/143‑1 (MQ=255)
                                                                                                          tGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAAt                                        <  1:72342/146‑1 (MQ=255)
                                                                                                           gCTGATGCCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATctct                                    <  2:714409/149‑1 (MQ=255)
                                                                                                           gCTGATGACCACCTCGATGAAGGGATCGATATCGTGAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATctct                                    <  2:1335066/149‑1 (MQ=255)
                                                                                                           gCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCCAAATCTTAGTCGCTGAATTGAAAAGCGGAATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCCGTAAtc                                       >  1:71908/1‑146 (MQ=255)
                                                                                                            cTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAAtct                                      <  1:593324/146‑1 (MQ=255)
                                                                                                            cTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTg                                   <  2:473261/149‑1 (MQ=255)
                                                                                                             tgatgaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTgg                                  <  2:644123/149‑1 (MQ=255)
                                                                                                             tgatgaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTgg                                  <  2:1708652/149‑1 (MQ=255)
                                                                                                             tgatgaCCAACTCGAAGAGATGATCGATATCGTCAGATAGGCAGCAACCAACGAAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTgg                                  <  2:1563350/149‑1 (MQ=255)
                                                                                                                tgaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTgg                                  <  1:787705/146‑1 (MQ=255)
                                                                                                                 gaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCCACGCGGCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcacg                              >  2:496978/1‑148 (MQ=255)
                                                                                                                 gaCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGcaca                              >  2:1420769/1‑149 (MQ=255)
                                                                                                                  aCCAACTCGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGca                                >  1:886090/1‑146 (MQ=255)
                                                                                                                   ccAACTCGATGAAGTGATCGAAATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTCATCTCTGGcacaca                            >  2:930147/1‑149 (MQ=255)
                                                                                                                    cAACTCGAGGAAGTGATCGATAGCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAg                           <  2:1398511/149‑1 (MQ=255)
                                                                                                                      cccccaTGAAGTGATGGATATCGTCAGTTAGGCGGCTTACTTCTGAAAAATTGGCGACTGCAAAATTTTTGTCGCTGAATTGCAACGCGTCATTCCTATTCGTACCGGCGAAGCCGTCGAAGCGGCGCTGTAATCTCTGGCACACAGCa                         <  2:354103/144‑1 (MQ=255)
                                                                                                                         cGATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACa                      <  2:138886/149‑1 (MQ=255)
                                                                                                                          gATGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAg                     >  2:1171259/1‑149 (MQ=255)
                                                                                                                           aTGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAAc                       <  1:1661477/146‑1 (MQ=255)
                                                                                                                           aTGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAgg                    >  2:1241634/1‑149 (MQ=255)
                                                                                                                            tGAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCGTTGCCATCTCCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGa                   <  2:1743797/149‑1 (MQ=255)
                                                                                                                             gAAGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGaa                  <  2:1691073/149‑1 (MQ=255)
                                                                                                                              aaGTGGTCGATATCGTCAGGTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAAc                 <  2:524374/149‑1 (MQ=255)
                                                                                                                               cgTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACg                <  2:671119/148‑1 (MQ=255)
                                                                                                                               aGTGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGACGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACg                >  2:127265/1‑149 (MQ=255)
                                                                                                                                 tGATCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACCCCGCAACAGGAAc                 >  1:629911/1‑146 (MQ=255)
                                                                                                                                   aTCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCAAAGCCGCCGAAGCGGCGCTGCAAGCGCTGGCACACAGCCACATGAACgg               >  1:324593/1‑145 (MQ=255)
                                                                                                                                    tCGATGTCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGaa              <  1:5550/146‑1 (MQ=255)
                                                                                                                                    tCGATATCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGACCGaaaaa           >  2:190118/1‑149 (MQ=255)
                                                                                                                                       atatCGTCAGTTAGGCGGCTTACACCGGAATAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCAGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGa        >  2:924080/1‑149 (MQ=255)
                                                                                                                                       atatCGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGACGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGa        >  2:1380216/1‑149 (MQ=255)
                                                                                                                                          ccGTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGGTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGa        <  1:619818/145‑1 (MQ=255)
                                                                                                                                            gTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATa   <  2:1738344/149‑1 (MQ=255)
                                                                                                                                            gTCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATGCGTATTCGTAGCGGCGAAGCCGACGAAGCGGCGCTGTAAGCGCTGGCACACAGCAACAGGAACGAAAAAGGAAg      >  1:1246190/1‑146 (MQ=255)
                                                                                                                                             tCAGTTAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAg  <  2:411083/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TCACCGAAGTGAAAGGTTTCGGGCGTCAGAAAGGGCATGCCGAGCTGTACCGGGGGGCGGAATACAGCGTCAATTTCCTGCCAAAAGTAAAAATTGATGTGGCGATTGCTGATGACCAACTCGATGAAGTGATCGATATCGTCAGTAAGGCGGCTTACACCGGAAAAATTGGCGACGGCAAAATCTTCGTCGCTGAATTGCAACGCGTCATTCGTATTCGTACCGGCGAAGCCGACGAAGCGGCGCTGTAATCTCTGGCACACAGCAACAGGAACGAAAAATGAAGATAG  >  CP009273/468142‑468431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: